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The map label for this gene is folC [H]

Identifier: 162147047

GI number: 162147047

Start: 1246246

End: 1247544

Strand: Reverse

Name: folC [H]

Synonym: GDI_1252

Alternate gene names: 162147047

Gene position: 1247544-1246246 (Counterclockwise)

Preceding gene: 162147048

Following gene: 162147046

Centisome position: 31.63

GC content: 73.6

Gene sequence:

>1299_bases
ATGCCCGCCACCTCGCTGGGGCCGGAATTCGTCGGGCGCGCCGGCGCGGTGCTGGAGCGGCTGAACCGGCTGTACCCCGC
CCTGATCGATCTGTCGCTGGGACGGCTGCAGGCGCTGCTGGCGCGGCTGGGCCACCCGGAACGCCACCTGCCGCCGGTGA
TCCATGTCGCCGGCACCAACGGCAAGGGCAGCACCTGCGCCACCCTGCGCGCCATCGGCGAACAGGCGGGCTGGCCGGTT
CACGTCATGACCTCGCCGCACCTGGTGGACGTCACCGAGCGCTTCCGCGTGGCCGGCCACATCGTGTCCACCGACGCGCT
GGTCGCGGTGCTGGAGGAGATCGAGCGCGTCAATGACGGAGCGCCGATCACGGTGTTCGAGGTCCTGACCGCCGCCGGCT
TCCTGCTGTTCGCCCGCCATCCCGCGCGCCTGGCGATCATCGAGGTCGGGCTGGGCGGCCGCTTCGATGCCACCAACGTG
CTGGAAAAGCCCGCCGCCTGCGCGATCACGTCGATTTCCATGGATCACGAGGCCTTTCTGGGCGACAGCCTGGCCGCCAT
CGCCGCCGAGAAGGCCGGGATCATGAAGCCCGGCGTACCTGTCGTAACCGGCCGCCAGGCGGAGGCCGCGATGGCGGTCC
TGCGGGCGCAGGCCGCGCAATGCGGCGCGCCCCTGCGGGTCCGGGGCGAGGACTGGGACATTGCCGCCACGCCGGGCGGC
CTGCGCTATACCGACGCGCGGGGCGCCCTGGACCTGCCGCTGCCCGGCCTGCCGGGGGCGCACCAGATCGACAATGCCGG
GCTGGCCGTGGCGGCGCTGCGTGCCAGCGGGCTGGAGGTGCCCGATTCGGCGTGGGCCGGGATTGCCCATACCGTGTGGC
CGGCGCGCATGCAGCGCCTGGGCGGGGCGCTGGCCGGTCTGCTGCCGCCGGGGTGGGAATTATGGCTGGATGGCGGGCAC
AATCCCGGCGCCGGGCAGGCCCTGGCGGACGTGTTGGCCGGCTGGTCCGACCGGCCGGTGCATCTGGTGATCGGCATGAA
GCAGACCAAGGACGCCGCCGGCTTCCTGTCCCCCATGCTGCCCCATGCCGCCAGCGTGCAGGCGGTGGCCGAGGCGGGCC
AGCATCTGGCCCTGCCGGTCGAGGCCATCATCGCCGCGTCGTCCGGGCGGGCCACGCCCGGCCCCACCCTGCGCGCGGCG
CTGGGACGGCTGACGGGCGAGCCGGCGCGGGTGGTGATCTGCGGCAGCCTGTATCTGGCGGGGGTGGCGCTGGAACAGGA
TGGGTGGCGGGCGGGTTAA

Upstream 100 bases:

>100_bases
GGGGCGGCTGATCGGCCTGATGAACCATCGGCATGTCAACGCCCCGGGAGCCGCGCTGGGCGGGGCCGCGCCGGTTCGGC
CGGCGGCCTGATCCGCGCCG

Downstream 100 bases:

>100_bases
GGCCACCACCATGAAGGTCATCTTCGACACCGACCCCGGCGTGGACGACGCCATGGCGCTGCGGTTCCTGACCCGCCGGC
CGGAATTCGAGCTGCTGGCC

Product: folylpoly-gamma-glutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPATSLGPEFVGRAGAVLERLNRLYPALIDLSLGRLQALLARLGHPERHLPPVIHVAGTNGKGSTCATLRAIGEQAGWPV
HVMTSPHLVDVTERFRVAGHIVSTDALVAVLEEIERVNDGAPITVFEVLTAAGFLLFARHPARLAIIEVGLGGRFDATNV
LEKPAACAITSISMDHEAFLGDSLAAIAAEKAGIMKPGVPVVTGRQAEAAMAVLRAQAAQCGAPLRVRGEDWDIAATPGG
LRYTDARGALDLPLPGLPGAHQIDNAGLAVAALRASGLEVPDSAWAGIAHTVWPARMQRLGGALAGLLPPGWELWLDGGH
NPGAGQALADVLAGWSDRPVHLVIGMKQTKDAAGFLSPMLPHAASVQAVAEAGQHLALPVEAIIAASSGRATPGPTLRAA
LGRLTGEPARVVICGSLYLAGVALEQDGWRAG

Sequences:

>Translated_432_residues
MPATSLGPEFVGRAGAVLERLNRLYPALIDLSLGRLQALLARLGHPERHLPPVIHVAGTNGKGSTCATLRAIGEQAGWPV
HVMTSPHLVDVTERFRVAGHIVSTDALVAVLEEIERVNDGAPITVFEVLTAAGFLLFARHPARLAIIEVGLGGRFDATNV
LEKPAACAITSISMDHEAFLGDSLAAIAAEKAGIMKPGVPVVTGRQAEAAMAVLRAQAAQCGAPLRVRGEDWDIAATPGG
LRYTDARGALDLPLPGLPGAHQIDNAGLAVAALRASGLEVPDSAWAGIAHTVWPARMQRLGGALAGLLPPGWELWLDGGH
NPGAGQALADVLAGWSDRPVHLVIGMKQTKDAAGFLSPMLPHAASVQAVAEAGQHLALPVEAIIAASSGRATPGPTLRAA
LGRLTGEPARVVICGSLYLAGVALEQDGWRAG
>Mature_431_residues
PATSLGPEFVGRAGAVLERLNRLYPALIDLSLGRLQALLARLGHPERHLPPVIHVAGTNGKGSTCATLRAIGEQAGWPVH
VMTSPHLVDVTERFRVAGHIVSTDALVAVLEEIERVNDGAPITVFEVLTAAGFLLFARHPARLAIIEVGLGGRFDATNVL
EKPAACAITSISMDHEAFLGDSLAAIAAEKAGIMKPGVPVVTGRQAEAAMAVLRAQAAQCGAPLRVRGEDWDIAATPGGL
RYTDARGALDLPLPGLPGAHQIDNAGLAVAALRASGLEVPDSAWAGIAHTVWPARMQRLGGALAGLLPPGWELWLDGGHN
PGAGQALADVLAGWSDRPVHLVIGMKQTKDAAGFLSPMLPHAASVQAVAEAGQHLALPVEAIIAASSGRATPGPTLRAAL
GRLTGEPARVVICGSLYLAGVALEQDGWRAG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=310, Percent_Identity=33.5483870967742, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI66932984, Length=310, Percent_Identity=33.5483870967742, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1788654, Length=344, Percent_Identity=40.1162790697674, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17553148, Length=300, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=300, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=300, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=428, Percent_Identity=29.6728971962617, Blast_Score=186, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6324815, Length=306, Percent_Identity=27.7777777777778, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322718, Length=422, Percent_Identity=22.9857819905213, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=33.5195530726257, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24581568, Length=177, Percent_Identity=35.0282485875706, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44634; Mature: 44503

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATSLGPEFVGRAGAVLERLNRLYPALIDLSLGRLQALLARLGHPERHLPPVIHVAGTN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
GKGSTCATLRAIGEQAGWPVHVMTSPHLVDVTERFRVAGHIVSTDALVAVLEEIERVNDG
CCCCHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHHEECHHHHHHHHHHHHHHCCC
APITVFEVLTAAGFLLFARHPARLAIIEVGLGGRFDATNVLEKPAACAITSISMDHEAFL
CCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCHHHHH
GDSLAAIAAEKAGIMKPGVPVVTGRQAEAAMAVLRAQAAQCGAPLRVRGEDWDIAATPGG
CCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCC
LRYTDARGALDLPLPGLPGAHQIDNAGLAVAALRASGLEVPDSAWAGIAHTVWPARMQRL
CEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCHHHCCHHHHHHHHHHHHH
GGALAGLLPPGWELWLDGGHNPGAGQALADVLAGWSDRPVHLVIGMKQTKDAAGFLSPML
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHC
PHAASVQAVAEAGQHLALPVEAIIAASSGRATPGPTLRAALGRLTGEPARVVICGSLYLA
CCHHHHHHHHHCCCEEECCHHHHEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCEEE
GVALEQDGWRAG
EEEEECCCCCCC
>Mature Secondary Structure 
PATSLGPEFVGRAGAVLERLNRLYPALIDLSLGRLQALLARLGHPERHLPPVIHVAGTN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
GKGSTCATLRAIGEQAGWPVHVMTSPHLVDVTERFRVAGHIVSTDALVAVLEEIERVNDG
CCCCHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHHEECHHHHHHHHHHHHHHCCC
APITVFEVLTAAGFLLFARHPARLAIIEVGLGGRFDATNVLEKPAACAITSISMDHEAFL
CCCHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEECCCHHHHH
GDSLAAIAAEKAGIMKPGVPVVTGRQAEAAMAVLRAQAAQCGAPLRVRGEDWDIAATPGG
CCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCC
LRYTDARGALDLPLPGLPGAHQIDNAGLAVAALRASGLEVPDSAWAGIAHTVWPARMQRL
CEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCHHHCCHHHHHHHHHHHHH
GGALAGLLPPGWELWLDGGHNPGAGQALADVLAGWSDRPVHLVIGMKQTKDAAGFLSPML
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHC
PHAASVQAVAEAGQHLALPVEAIIAASSGRATPGPTLRAALGRLTGEPARVVICGSLYLA
CCHHHHHHHHHCCCEEECCHHHHEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCEEE
GVALEQDGWRAG
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]