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The map label for this gene is nsnH [H]

Identifier: 162147046

GI number: 162147046

Start: 1245279

End: 1246235

Strand: Reverse

Name: nsnH [H]

Synonym: GDI_1251

Alternate gene names: 162147046

Gene position: 1246235-1245279 (Counterclockwise)

Preceding gene: 162147047

Following gene: 162147042

Centisome position: 31.6

GC content: 71.79

Gene sequence:

>957_bases
ATGAAGGTCATCTTCGACACCGACCCCGGCGTGGACGACGCCATGGCGCTGCGGTTCCTGACCCGCCGGCCGGAATTCGA
GCTGCTGGCCATCACCACGGTGCACGGCAACCTGGACGTCGATACCGTCACCCGCAACGCGCTGTGGCTGGCCGGCTGGA
TGGGGCTGTCCGTGCCGGTGGCACGCGGGGCGGAGGCGCCGCTCTGCCGGGACGCGGGCCGGGGCGCGCCGCATGTGCAT
GGCGCGGGCGGGCTGGGCGACCTGACCCTGCCCGACCCGCTGGACCGTACCGCCGATCCCCGCCCCGCCTGGCAGGTCAT
CGTCGACCTGGTGCGCGCCTATCCCCACCAGGTCACGCTGATCGCGGTCGGGCCGCTGACCAACCTGGCGCTGGCACTGC
GGCATGACCCCGCCATTGCCGGACTGGTGGCCGGCGTCAGCCTGATGGGCGGGGCGTTCGGCATGCACGGCCATACCGGC
AACATGTCCCCGGTGGCCGAGGCCAACATCCTGTCCGACCCCGAAGCCGCGGATCAGGTCATGACCGCGCCCTGGCCGGT
GTCGATCCTGGGGCTGGACGTGACGCAGCAGGTGGTGATGGACGCGGCCTACCTGGACCGGCTGGGCGCGCAGGGCGGAG
CGGACGGCGCCTTCCTGCGCGGGGTGTCACGCTTCTACCAGGCGTTTCACGAGCGCTCGCGCGGGCTGGCGGGGATCTTC
GCGCATGACAGCCTGGCGGTGATCCATGCCATGGCGCCCCATTTGTTCACCCTGCGCCACGGACCGGTCCGGGTGGTCTG
CGGGGGACTGGCGGACGGCCAGACCATTCAGAAGCCCGACGGTTTTCCCTGCCCCCCCACCGCCGCGTGGGACGCGTTTC
CGGCGCGGGCCGTGGCGGTGGACGTGGATGTCGCGGGTGTCCTGGCGTTGTATGCGGGGACGTTCGGGGTGTCCTGA

Upstream 100 bases:

>100_bases
CTGACGGGCGAGCCGGCGCGGGTGGTGATCTGCGGCAGCCTGTATCTGGCGGGGGTGGCGCTGGAACAGGATGGGTGGCG
GGCGGGTTAAGGCCACCACC

Downstream 100 bases:

>100_bases
TCCGTTCAGGGACGATGGTCCGGATGCATCGGGCCGGCGGCGCGGACCCGTCCGACCGTCATGCCGTTCCAGTTGCGCAG
GTCATGCTGCGCCAGGCCCC

Product: inosine-uridine preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKVIFDTDPGVDDAMALRFLTRRPEFELLAITTVHGNLDVDTVTRNALWLAGWMGLSVPVARGAEAPLCRDAGRGAPHVH
GAGGLGDLTLPDPLDRTADPRPAWQVIVDLVRAYPHQVTLIAVGPLTNLALALRHDPAIAGLVAGVSLMGGAFGMHGHTG
NMSPVAEANILSDPEAADQVMTAPWPVSILGLDVTQQVVMDAAYLDRLGAQGGADGAFLRGVSRFYQAFHERSRGLAGIF
AHDSLAVIHAMAPHLFTLRHGPVRVVCGGLADGQTIQKPDGFPCPPTAAWDAFPARAVAVDVDVAGVLALYAGTFGVS

Sequences:

>Translated_318_residues
MKVIFDTDPGVDDAMALRFLTRRPEFELLAITTVHGNLDVDTVTRNALWLAGWMGLSVPVARGAEAPLCRDAGRGAPHVH
GAGGLGDLTLPDPLDRTADPRPAWQVIVDLVRAYPHQVTLIAVGPLTNLALALRHDPAIAGLVAGVSLMGGAFGMHGHTG
NMSPVAEANILSDPEAADQVMTAPWPVSILGLDVTQQVVMDAAYLDRLGAQGGADGAFLRGVSRFYQAFHERSRGLAGIF
AHDSLAVIHAMAPHLFTLRHGPVRVVCGGLADGQTIQKPDGFPCPPTAAWDAFPARAVAVDVDVAGVLALYAGTFGVS
>Mature_318_residues
MKVIFDTDPGVDDAMALRFLTRRPEFELLAITTVHGNLDVDTVTRNALWLAGWMGLSVPVARGAEAPLCRDAGRGAPHVH
GAGGLGDLTLPDPLDRTADPRPAWQVIVDLVRAYPHQVTLIAVGPLTNLALALRHDPAIAGLVAGVSLMGGAFGMHGHTG
NMSPVAEANILSDPEAADQVMTAPWPVSILGLDVTQQVVMDAAYLDRLGAQGGADGAFLRGVSRFYQAFHERSRGLAGIF
AHDSLAVIHAMAPHLFTLRHGPVRVVCGGLADGQTIQKPDGFPCPPTAAWDAFPARAVAVDVDVAGVLALYAGTFGVS

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=276, Percent_Identity=36.231884057971, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=36.2459546925566, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786871, Length=261, Percent_Identity=33.7164750957854, Blast_Score=127, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32565474, Length=274, Percent_Identity=31.021897810219, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565698, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320608, Length=187, Percent_Identity=34.7593582887701, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24641839, Length=272, Percent_Identity=31.6176470588235, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583915, Length=208, Percent_Identity=33.6538461538462, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24641837, Length=197, Percent_Identity=35.5329949238579, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 33329; Mature: 33329

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIFDTDPGVDDAMALRFLTRRPEFELLAITTVHGNLDVDTVTRNALWLAGWMGLSVPV
CEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHCCCEEEEHHHCCCCCC
ARGAEAPLCRDAGRGAPHVHGAGGLGDLTLPDPLDRTADPRPAWQVIVDLVRAYPHQVTL
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
IAVGPLTNLALALRHDPAIAGLVAGVSLMGGAFGMHGHTGNMSPVAEANILSDPEAADQV
EEECCHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHE
MTAPWPVSILGLDVTQQVVMDAAYLDRLGAQGGADGAFLRGVSRFYQAFHERSRGLAGIF
ECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
AHDSLAVIHAMAPHLFTLRHGPVRVVCGGLADGQTIQKPDGFPCPPTAAWDAFPARAVAV
ECCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DVDVAGVLALYAGTFGVS
ECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVIFDTDPGVDDAMALRFLTRRPEFELLAITTVHGNLDVDTVTRNALWLAGWMGLSVPV
CEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHCCCEEEEHHHCCCCCC
ARGAEAPLCRDAGRGAPHVHGAGGLGDLTLPDPLDRTADPRPAWQVIVDLVRAYPHQVTL
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
IAVGPLTNLALALRHDPAIAGLVAGVSLMGGAFGMHGHTGNMSPVAEANILSDPEAADQV
EEECCHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHE
MTAPWPVSILGLDVTQQVVMDAAYLDRLGAQGGADGAFLRGVSRFYQAFHERSRGLAGIF
ECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
AHDSLAVIHAMAPHLFTLRHGPVRVVCGGLADGQTIQKPDGFPCPPTAAWDAFPARAVAV
ECCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
DVDVAGVLALYAGTFGVS
ECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA