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The map label for this gene is ogt [H]

Identifier: 162147025

GI number: 162147025

Start: 1223140

End: 1223631

Strand: Direct

Name: ogt [H]

Synonym: GDI_1230

Alternate gene names: 162147025

Gene position: 1223140-1223631 (Clockwise)

Preceding gene: 162147017

Following gene: 162147026

Centisome position: 31.01

GC content: 67.89

Gene sequence:

>492_bases
ATGACCTGGGATACGACGATGCCGCAGCTTTCCATGCACTCGCCCATCGGCGACCTGACCCTGACCGAGGAAGACGGGGC
GATCATCGCGCTGGACTGGGGATGGGGCCGCGACCAGGACGAAAACCCCCTGCTGCGCCACGCCTGCGATCGGCTGGACG
CCTATTTCGACGGCGCGCCGGACCCGTTCGCCGACCTGGTCCTGGCACCGCACGGCACCGCCTACCAGCGCACGGTATGG
CAGGCGGTGCGCGCCATACCCCTGGGCCAGACCCGGACCTATCGGCAGATCGCGCAGATCGCCGGCGGTTCGCCCCGATC
GGTCGGGAACGCCAACAGCGTCAATCCCATTCCCATCCTGATTCCCTGCCATCGCGTCGTCTCCGTCCAGGGCCTGGGCG
GCTATTCCGGCGATGGCGGGTTGACCGACAAGCTCTATCTTCTGGGCCTGGAACGCGCCCCCATTCCAGCCCACGCGCAC
GAGACGGCCTGA

Upstream 100 bases:

>100_bases
TGTCCCGGCGTGTGCGTGATCCCTCTTGCATGTCGCATCCTGCGGCGGAAGAGTACAGAATACGCGACCGAACCGATATG
CGCGTTCCGCACCGTGGCGG

Downstream 100 bases:

>100_bases
CACAGCCACCACGGAAAGGAAGAGAGCGATGTACAGGGCGATTCGCATCCATGATTACGGCGGGCCCGAAGTCCTGAAGC
TGGAAACGCTGCCCAGCCCC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTWDTTMPQLSMHSPIGDLTLTEEDGAIIALDWGWGRDQDENPLLRHACDRLDAYFDGAPDPFADLVLAPHGTAYQRTVW
QAVRAIPLGQTRTYRQIAQIAGGSPRSVGNANSVNPIPILIPCHRVVSVQGLGGYSGDGGLTDKLYLLGLERAPIPAHAH
ETA

Sequences:

>Translated_163_residues
MTWDTTMPQLSMHSPIGDLTLTEEDGAIIALDWGWGRDQDENPLLRHACDRLDAYFDGAPDPFADLVLAPHGTAYQRTVW
QAVRAIPLGQTRTYRQIAQIAGGSPRSVGNANSVNPIPILIPCHRVVSVQGLGGYSGDGGLTDKLYLLGLERAPIPAHAH
ETA
>Mature_162_residues
TWDTTMPQLSMHSPIGDLTLTEEDGAIIALDWGWGRDQDENPLLRHACDRLDAYFDGAPDPFADLVLAPHGTAYQRTVWQ
AVRAIPLGQTRTYRQIAQIAGGSPRSVGNANSVNPIPILIPCHRVVSVQGLGGYSGDGGLTDKLYLLGLERAPIPAHAHE
TA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=175, Percent_Identity=32.5714285714286, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=44.1176470588235, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1788542, Length=84, Percent_Identity=45.2380952380952, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17620; Mature: 17489

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWDTTMPQLSMHSPIGDLTLTEEDGAIIALDWGWGRDQDENPLLRHACDRLDAYFDGAP
CCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DPFADLVLAPHGTAYQRTVWQAVRAIPLGQTRTYRQIAQIAGGSPRSVGNANSVNPIPIL
CCHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IPCHRVVSVQGLGGYSGDGGLTDKLYLLGLERAPIPAHAHETA
EECCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TWDTTMPQLSMHSPIGDLTLTEEDGAIIALDWGWGRDQDENPLLRHACDRLDAYFDGAP
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DPFADLVLAPHGTAYQRTVWQAVRAIPLGQTRTYRQIAQIAGGSPRSVGNANSVNPIPIL
CCHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IPCHRVVSVQGLGGYSGDGGLTDKLYLLGLERAPIPAHAHETA
EECCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA