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The map label for this gene is yieH [H]

Identifier: 162147009

GI number: 162147009

Start: 1204294

End: 1205031

Strand: Reverse

Name: yieH [H]

Synonym: GDI_1214

Alternate gene names: 162147009

Gene position: 1205031-1204294 (Counterclockwise)

Preceding gene: 162147010

Following gene: 162147008

Centisome position: 30.55

GC content: 69.24

Gene sequence:

>738_bases
ATGGAGCCCGGAACATCATGGCCCGCGGCGACGCTGCCCCGGTCGGTCCGGCCGGACGGCCGGCTGCGGCTGGTCATCTT
CGATTGCGACGGCGTGCTGATCGACAGCGAGGGCCCGTCCTGCCGTGTCGTGGCACGGGAACTGCGCCAGATCGGCGTCG
AACTGGATGACGAAGCGGCGGTGGAACGCCTGGCCGGACGGGCCCTGACGCGGATCAAGACCGAAATGGAGGCCGAAACC
GGCCGCGTGCTGCCCGATGACTGGGCGGCGGTGGTGCAGCAGAAGCTGGTCGAACTGATGCAGCGGGAGGCCCGCGTCAT
CGATGGCGCGCACGGCATGCTGACGGCGGTGATCGGCCTCGACCTGCCGGTGCGGGTCGGTTCCAACTCCTCGATCGCTG
AGATGGACGTGAAATTCGCCCGGACCGGGCTGGATCGCTTCGTCGCCGACCGGATTCACTCGGCGCGCGACATGGGCAAG
CCGAAGCCCGATCCGGCCGTCTACCTGCATGCCGCCGAAATCGAAGGCGTTGCCCCGGACGAATGCATCGTCCTGGAAGA
TACCGACACCGGCGCCAGCGCCGCCCGGGCGGCCGGCATGGCCTGCGTGCTGCTGCGCCCGCTGGACCTGCCGGCGCCCG
ACTGGCCGGGGCTGTTCCGTATCGCGCATCTGTCCGAATTCGCGCCGTTGCTGGAACGGATCCTGGCGGCGCAGGCATCA
GGCGGAACACGGTCTTGA

Upstream 100 bases:

>100_bases
AAGGGCGGCCGCACGTCTTCAATCTCGGCCACGGCGTGGTGCCCCCGACGCCGCCCGAACATGTCGGTGATCTTGTGCGC
ACCGTCAGGGAGGTCTGAGC

Downstream 100 bases:

>100_bases
TCCTGGGTTTCCTGCCCTGGCTGGCATGGTTCAAGGCGCTGCATGTGATGTCGCTGATCGCATGGATGGCCGGCCTGTTC
TACCTGCCGCGCCTGTTCGT

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MEPGTSWPAATLPRSVRPDGRLRLVIFDCDGVLIDSEGPSCRVVARELRQIGVELDDEAAVERLAGRALTRIKTEMEAET
GRVLPDDWAAVVQQKLVELMQREARVIDGAHGMLTAVIGLDLPVRVGSNSSIAEMDVKFARTGLDRFVADRIHSARDMGK
PKPDPAVYLHAAEIEGVAPDECIVLEDTDTGASAARAAGMACVLLRPLDLPAPDWPGLFRIAHLSEFAPLLERILAAQAS
GGTRS

Sequences:

>Translated_245_residues
MEPGTSWPAATLPRSVRPDGRLRLVIFDCDGVLIDSEGPSCRVVARELRQIGVELDDEAAVERLAGRALTRIKTEMEAET
GRVLPDDWAAVVQQKLVELMQREARVIDGAHGMLTAVIGLDLPVRVGSNSSIAEMDVKFARTGLDRFVADRIHSARDMGK
PKPDPAVYLHAAEIEGVAPDECIVLEDTDTGASAARAAGMACVLLRPLDLPAPDWPGLFRIAHLSEFAPLLERILAAQAS
GGTRS
>Mature_245_residues
MEPGTSWPAATLPRSVRPDGRLRLVIFDCDGVLIDSEGPSCRVVARELRQIGVELDDEAAVERLAGRALTRIKTEMEAET
GRVLPDDWAAVVQQKLVELMQREARVIDGAHGMLTAVIGLDLPVRVGSNSSIAEMDVKFARTGLDRFVADRIHSARDMGK
PKPDPAVYLHAAEIEGVAPDECIVLEDTDTGASAARAAGMACVLLRPLDLPAPDWPGLFRIAHLSEFAPLLERILAAQAS
GGTRS

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=214, Percent_Identity=29.9065420560748, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789046, Length=192, Percent_Identity=32.2916666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 26391; Mature: 26391

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPGTSWPAATLPRSVRPDGRLRLVIFDCDGVLIDSEGPSCRVVARELRQIGVELDDEAA
CCCCCCCCHHHCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCHHHH
VERLAGRALTRIKTEMEAETGRVLPDDWAAVVQQKLVELMQREARVIDGAHGMLTAVIGL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DLPVRVGSNSSIAEMDVKFARTGLDRFVADRIHSARDMGKPKPDPAVYLHAAEIEGVAPD
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHCCCCCCCC
ECIVLEDTDTGASAARAAGMACVLLRPLDLPAPDWPGLFRIAHLSEFAPLLERILAAQAS
CEEEEECCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GGTRS
CCCCC
>Mature Secondary Structure
MEPGTSWPAATLPRSVRPDGRLRLVIFDCDGVLIDSEGPSCRVVARELRQIGVELDDEAA
CCCCCCCCHHHCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCCHHHH
VERLAGRALTRIKTEMEAETGRVLPDDWAAVVQQKLVELMQREARVIDGAHGMLTAVIGL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DLPVRVGSNSSIAEMDVKFARTGLDRFVADRIHSARDMGKPKPDPAVYLHAAEIEGVAPD
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHCCCCCCCC
ECIVLEDTDTGASAARAAGMACVLLRPLDLPAPDWPGLFRIAHLSEFAPLLERILAAQAS
CEEEEECCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GGTRS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]