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The map label for this gene is afr [H]

Identifier: 162146995

GI number: 162146995

Start: 1194369

End: 1195373

Strand: Reverse

Name: afr [H]

Synonym: GDI_1200

Alternate gene names: 162146995

Gene position: 1195373-1194369 (Counterclockwise)

Preceding gene: 162146996

Following gene: 162146994

Centisome position: 30.31

GC content: 65.07

Gene sequence:

>1005_bases
ATGACGCATCCGACCGGATGGGGTCTGATAGGGGCAAGCAACGTCGCGCGGGAGTGGATCATCGACGCGATCCGCGCCCA
GCCCGGCAGCGACGTGCGCGGAGTGCTCAGCAGCGATGCCGGGCGCGGTGCGGCGTTCGCCGAGGCCAACGGCATCGCCT
ATCACACGACGTCCCTTCAGGACCTGCTGTCCAGGCCGGACATCGACGCGGTCTATATCAGTACCAACAACCGCCTGCAT
TTCGAACAGACCATCGCCGCCGCGCGGGCGGGCAAGCATGTCATGTGCGAAAAGCCGCTGGCCCTGTCGGTCGATGACGC
GAGCGAGATGGTGCGCGTCTGCCGCCAGTCCAACGTCGTGCTGGGAACCAACCACCATCTGCGCAATGCCGCGACCCACA
TGGCCATCCATGGCCTGATCGAGGACGGGCGGATCGGCGGCCTGATCGCGGCCCGGTTGTTTCACGCCAATTTCCTGGCC
GCCGCGCTGCAGGGATGGCGCGTCAACGACCAGGGTGGCGGGGTGATCCTGGACAGCACGATCCACGATATCGACACGCT
GCGTTTCCTGTTCGATGAAAACCCCGTCAGCGTCTTCGCCATGACGCAGAGCGGGCGCCTGGCGCAGGACGGGATCGAGG
ACGGCATCATGGCCGTCCTGCGCTTTCCCGGCGATGTCCTGGTGCAGATTCATGGCGGATATACCACGCCATTTTCACCG
GGCGGCGTGGAGATCATGGGCGAGCGCGGCATCATCATCGGCCGCGATTGCATGAGCCAGCGGCCCATCGGCACCGTATC
GGTGCGCGACGGCGAGGGCGAACATGACATACCGTTGAAGCATCATAACCTTTATCATCGCGCCTTCGCCCTGTTCACCG
CCGCCATCCATGGTGACGGCCGTCCCGCCGCCACGGGCGAGGACGGTGTGGCATCGCTGGGTATTGCGCTGGCCCTGCGT
GAATCCGCCCGCACCGGACGTGAAATCGGGATCAACCTGCCATGA

Upstream 100 bases:

>100_bases
CGTTCCGCCGCGCGGTATTCTGCCGGTCGCTCATCCGGGTCTTTACGACAATCAATAATACGAACAAGGACCGGGGCCTG
GTGCCTCGAAGGAGGACGGT

Downstream 100 bases:

>100_bases
AACAGCCAGTTCTCGCCGGGGTGGCCCAGGCAGGCGTCCCCCCGGGCCGGTCCCGCACGCCGAACCGCGAGATGGAGCTG
TCGCGCCCCGAGGTCACCAC

Product: oxidoreductase protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTHPTGWGLIGASNVAREWIIDAIRAQPGSDVRGVLSSDAGRGAAFAEANGIAYHTTSLQDLLSRPDIDAVYISTNNRLH
FEQTIAAARAGKHVMCEKPLALSVDDASEMVRVCRQSNVVLGTNHHLRNAATHMAIHGLIEDGRIGGLIAARLFHANFLA
AALQGWRVNDQGGGVILDSTIHDIDTLRFLFDENPVSVFAMTQSGRLAQDGIEDGIMAVLRFPGDVLVQIHGGYTTPFSP
GGVEIMGERGIIIGRDCMSQRPIGTVSVRDGEGEHDIPLKHHNLYHRAFALFTAAIHGDGRPAATGEDGVASLGIALALR
ESARTGREIGINLP

Sequences:

>Translated_334_residues
MTHPTGWGLIGASNVAREWIIDAIRAQPGSDVRGVLSSDAGRGAAFAEANGIAYHTTSLQDLLSRPDIDAVYISTNNRLH
FEQTIAAARAGKHVMCEKPLALSVDDASEMVRVCRQSNVVLGTNHHLRNAATHMAIHGLIEDGRIGGLIAARLFHANFLA
AALQGWRVNDQGGGVILDSTIHDIDTLRFLFDENPVSVFAMTQSGRLAQDGIEDGIMAVLRFPGDVLVQIHGGYTTPFSP
GGVEIMGERGIIIGRDCMSQRPIGTVSVRDGEGEHDIPLKHHNLYHRAFALFTAAIHGDGRPAATGEDGVASLGIALALR
ESARTGREIGINLP
>Mature_333_residues
THPTGWGLIGASNVAREWIIDAIRAQPGSDVRGVLSSDAGRGAAFAEANGIAYHTTSLQDLLSRPDIDAVYISTNNRLHF
EQTIAAARAGKHVMCEKPLALSVDDASEMVRVCRQSNVVLGTNHHLRNAATHMAIHGLIEDGRIGGLIAARLFHANFLAA
ALQGWRVNDQGGGVILDSTIHDIDTLRFLFDENPVSVFAMTQSGRLAQDGIEDGIMAVLRFPGDVLVQIHGGYTTPFSPG
GVEIMGERGIIIGRDCMSQRPIGTVSVRDGEGEHDIPLKHHNLYHRAFALFTAAIHGDGRPAATGEDGVASLGIALALRE
SARTGREIGINLP

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082405, Length=202, Percent_Identity=29.7029702970297, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787574, Length=196, Percent_Identity=29.0816326530612, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081947, Length=262, Percent_Identity=25.9541984732824, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581117, Length=226, Percent_Identity=26.9911504424779, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920536, Length=205, Percent_Identity=28.2926829268293, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581115, Length=195, Percent_Identity=23.5897435897436, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35678; Mature: 35547

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPTGWGLIGASNVAREWIIDAIRAQPGSDVRGVLSSDAGRGAAFAEANGIAYHTTSLQ
CCCCCCCCEECCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEECCCEEEEECCHH
DLLSRPDIDAVYISTNNRLHFEQTIAAARAGKHVMCEKPLALSVDDASEMVRVCRQSNVV
HHHCCCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEE
LGTNHHLRNAATHMAIHGLIEDGRIGGLIAARLFHANFLAAALQGWRVNDQGGGVILDST
EECCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECC
IHDIDTLRFLFDENPVSVFAMTQSGRLAQDGIEDGIMAVLRFPGDVLVQIHGGYTTPFSP
HHHHHHHHEEECCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GGVEIMGERGIIIGRDCMSQRPIGTVSVRDGEGEHDIPLKHHNLYHRAFALFTAAIHGDG
CCEEEEECCCEEECHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCC
RPAATGEDGVASLGIALALRESARTGREIGINLP
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCC
>Mature Secondary Structure 
THPTGWGLIGASNVAREWIIDAIRAQPGSDVRGVLSSDAGRGAAFAEANGIAYHTTSLQ
CCCCCCCEECCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEECCCEEEEECCHH
DLLSRPDIDAVYISTNNRLHFEQTIAAARAGKHVMCEKPLALSVDDASEMVRVCRQSNVV
HHHCCCCCCEEEEECCCEEEHHHHHHHHHCCCEEEECCCCEEEECCHHHHHHHHHHCCEE
LGTNHHLRNAATHMAIHGLIEDGRIGGLIAARLFHANFLAAALQGWRVNDQGGGVILDST
EECCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECC
IHDIDTLRFLFDENPVSVFAMTQSGRLAQDGIEDGIMAVLRFPGDVLVQIHGGYTTPFSP
HHHHHHHHEEECCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GGVEIMGERGIIIGRDCMSQRPIGTVSVRDGEGEHDIPLKHHNLYHRAFALFTAAIHGDG
CCEEEEECCCEEECHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCC
RPAATGEDGVASLGIALALRESARTGREIGINLP
CCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA