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The map label for this gene is mhpD [C]

Identifier: 162146993

GI number: 162146993

Start: 1192106

End: 1192792

Strand: Reverse

Name: mhpD [C]

Synonym: GDI_1198

Alternate gene names: 162146993

Gene position: 1192792-1192106 (Counterclockwise)

Preceding gene: 162146994

Following gene: 162146992

Centisome position: 30.24

GC content: 69.58

Gene sequence:

>687_bases
GTGCCCGAAGGGTGCGAGGTTCCCTCGGACGACGTGGCGTATCGGGTGCAGCATGCGACCATGCATGCCATGGGTGACCG
GATCGCGGGCTGGAAGGTCGGCGCCCGCACGCCGGATTCCGAACCGTTCGCGGCCCCGATCCTGGCCGCGACCCTGTTCG
ACGGCGAAACCGTGCTGCCGCCCGGCTTGTGCCGGCTGATCGGCGTCGAGGCCGAAATCGCCTATCGGTTCGGGCACGGC
CTGCCGCCGCGCGACGCGCCCTATACGACCGCGGAGGTGTGTGATGCGATCGTGTCGGTCCACACCGCGATCGAAATCAT
CGATACGCGCTTCGCCACCGCCGGCAGCCAGCCCGCGCTGGACCATCTGGCGGACCAGCAGAGCCACGGCGCCCTGTTCG
TCGGCCCGGGGATCACCGACTGGCGGTCCCTGGTACCGGTGGAGGAACGGGTGATCCTGACGGTCGGCGACAAGGTGGTG
GACGATCACGTCGGCGGCAATTCGGCCGGCGATCCGATCCGCACCCTGGTGTGGCTGGCGGCGCATGCCGCGCGCTATGC
CGGGGGACTGGCCGCCGGCACCATCGTCACCACCGGATCGACCACCGGCACCCTTTTCGTCGAACCCGGCACGCGGGTCA
GCGCGACATTCCCGCATCTGGGCACATTGTCGCTGACCTGCGGCTGA

Upstream 100 bases:

>100_bases
GCACAGGAGCATGTGTGTGACCGTTTCCACCGACCCACTGGTTGATCAACTGACTGAAGCCTTCCTGCGGGTCCGTGCCG
GCGCCCCCCCGCTGGCCCAC

Downstream 100 bases:

>100_bases
GTCCGCCTGCTCATTGGGGCCCATGGCGGGCCCTGAACCGGAGAACATGAATGAAACCCGATATTCTTCTGATCGAGCCG
ATGATGCCGCAGATCGAGAA

Product: 2-keto-4-pentenoate hydratase-like

Products: H2O; 2-oxopent-4-enoate [C]

Alternate protein names: 2-Keto-4-Pentenoate Hydratase-Like Protein; 2-Keto-4-Pentenoate Hydratase; 2-Keto-4-Pentenoate Hydratase-Like; Fumarylacetoacetate Hydrolase; 2-Oxopent-4-Enoate Hydratase; Hydratase/Decarboxylase Family Protein; Hydratase

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MPEGCEVPSDDVAYRVQHATMHAMGDRIAGWKVGARTPDSEPFAAPILAATLFDGETVLPPGLCRLIGVEAEIAYRFGHG
LPPRDAPYTTAEVCDAIVSVHTAIEIIDTRFATAGSQPALDHLADQQSHGALFVGPGITDWRSLVPVEERVILTVGDKVV
DDHVGGNSAGDPIRTLVWLAAHAARYAGGLAAGTIVTTGSTTGTLFVEPGTRVSATFPHLGTLSLTCG

Sequences:

>Translated_228_residues
MPEGCEVPSDDVAYRVQHATMHAMGDRIAGWKVGARTPDSEPFAAPILAATLFDGETVLPPGLCRLIGVEAEIAYRFGHG
LPPRDAPYTTAEVCDAIVSVHTAIEIIDTRFATAGSQPALDHLADQQSHGALFVGPGITDWRSLVPVEERVILTVGDKVV
DDHVGGNSAGDPIRTLVWLAAHAARYAGGLAAGTIVTTGSTTGTLFVEPGTRVSATFPHLGTLSLTCG
>Mature_227_residues
PEGCEVPSDDVAYRVQHATMHAMGDRIAGWKVGARTPDSEPFAAPILAATLFDGETVLPPGLCRLIGVEAEIAYRFGHGL
PPRDAPYTTAEVCDAIVSVHTAIEIIDTRFATAGSQPALDHLADQQSHGALFVGPGITDWRSLVPVEERVILTVGDKVVD
DHVGGNSAGDPIRTLVWLAAHAARYAGGLAAGTIVTTGSTTGTLFVEPGTRVSATFPHLGTLSLTCG

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081722, Length=235, Percent_Identity=25.531914893617, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.- [C]

Molecular weight: Translated: 23817; Mature: 23686

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGCEVPSDDVAYRVQHATMHAMGDRIAGWKVGARTPDSEPFAAPILAATLFDGETVLP
CCCCCCCCCCHHHEEHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCC
PGLCRLIGVEAEIAYRFGHGLPPRDAPYTTAEVCDAIVSVHTAIEIIDTRFATAGSQPAL
CHHHHHHCCCHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DHLADQQSHGALFVGPGITDWRSLVPVEERVILTVGDKVVDDHVGGNSAGDPIRTLVWLA
HHHHCCCCCCEEEECCCCHHHHHCCCCCCEEEEEECCEEHHHCCCCCCCCCHHHHHHHHH
AHAARYAGGLAAGTIVTTGSTTGTLFVEPGTRVSATFPHLGTLSLTCG
HHHHHHHCCCEECEEEECCCCCEEEEECCCCEEEEECCCCCEEEEECC
>Mature Secondary Structure 
PEGCEVPSDDVAYRVQHATMHAMGDRIAGWKVGARTPDSEPFAAPILAATLFDGETVLP
CCCCCCCCCHHHEEHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCC
PGLCRLIGVEAEIAYRFGHGLPPRDAPYTTAEVCDAIVSVHTAIEIIDTRFATAGSQPAL
CHHHHHHCCCHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DHLADQQSHGALFVGPGITDWRSLVPVEERVILTVGDKVVDDHVGGNSAGDPIRTLVWLA
HHHHCCCCCCEEEECCCCHHHHHCCCCCCEEEEEECCEEHHHCCCCCCCCCHHHHHHHHH
AHAARYAGGLAAGTIVTTGSTTGTLFVEPGTRVSATFPHLGTLSLTCG
HHHHHHHCCCEECEEEECCCCCEEEEECCCCEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-ketovalerate [C]

Specific reaction: 4-hydroxy-2-ketovalerate = H2O + 2-oxopent-4-enoate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA