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The map label for this gene is ptsO [H]

Identifier: 162146982

GI number: 162146982

Start: 1183999

End: 1184322

Strand: Reverse

Name: ptsO [H]

Synonym: GDI_1187

Alternate gene names: 162146982

Gene position: 1184322-1183999 (Counterclockwise)

Preceding gene: 162146983

Following gene: 162146981

Centisome position: 30.03

GC content: 67.9

Gene sequence:

>324_bases
ATGTCCTCGCCCGGCATGTCGGCTCCTGGCGGGCTGGACCCCCAGGACCCCGATGTCCTGTCCGTGACCCTGACCATGGT
CAATCGTCGCGGGCTGCATGCGCGCGCGGCGGCCAAGCTGGTGACGGTGGCCGAGCAGTTCGACGCGAATGTCGAGGTCA
CGCGGGCCGACCAGACCGTATCGGCGCGCTCGATCATGGGCCTGATGATGCTGGGGGCCGGTCGGGGGGAATCCATTACC
CTGATGGCCACGGGATGGGATGCCCATGCGGCGATCGAGGCCCTGGTGGCCCTGATCGAGGCGGGCTTCCATGAAGACGA
CTGA

Upstream 100 bases:

>100_bases
GGGCGCCCGGAGCTGCCTGCTGGGCGAACGCCCCGCCGACATGCCGCCGCAGGCGCTGTACCGTCCTGCGCCGCGGCGGG
TCGCCATGGGCTGACCGGGC

Downstream 100 bases:

>100_bases
CGCCGCCGGCCCCTCGTCCGCGCCGCGCCGGCGGGCTGCCGGCCGGCGGGGAACGCGGGCGGAACACCGGCTGTCCGGCG
ATGCCACGGTGCGCGGCATC

Product: phosphoryl transfer system, HPr

Products: NA

Alternate protein names: Nitrogen-related HPr [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MSSPGMSAPGGLDPQDPDVLSVTLTMVNRRGLHARAAAKLVTVAEQFDANVEVTRADQTVSARSIMGLMMLGAGRGESIT
LMATGWDAHAAIEALVALIEAGFHEDD

Sequences:

>Translated_107_residues
MSSPGMSAPGGLDPQDPDVLSVTLTMVNRRGLHARAAAKLVTVAEQFDANVEVTRADQTVSARSIMGLMMLGAGRGESIT
LMATGWDAHAAIEALVALIEAGFHEDD
>Mature_106_residues
SSPGMSAPGGLDPQDPDVLSVTLTMVNRRGLHARAAAKLVTVAEQFDANVEVTRADQTVSARSIMGLMMLGAGRGESITL
MATGWDAHAAIEALVALIEAGFHEDD

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the p

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1789599, Length=86, Percent_Identity=38.3720930232558, Blast_Score=59, Evalue=5e-11,
Organism=Escherichia coli, GI1788494, Length=95, Percent_Identity=37.8947368421053, Blast_Score=59, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 11163; Mature: 11032

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPGMSAPGGLDPQDPDVLSVTLTMVNRRGLHARAAAKLVTVAEQFDANVEVTRADQTV
CCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHH
SARSIMGLMMLGAGRGESITLMATGWDAHAAIEALVALIEAGFHEDD
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSPGMSAPGGLDPQDPDVLSVTLTMVNRRGLHARAAAKLVTVAEQFDANVEVTRADQTV
CCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHH
SARSIMGLMMLGAGRGESITLMATGWDAHAAIEALVALIEAGFHEDD
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10760597 [H]