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The map label for this gene is ptsI [H]

Identifier: 162146981

GI number: 162146981

Start: 1182156

End: 1184012

Strand: Reverse

Name: ptsI [H]

Synonym: GDI_1186

Alternate gene names: 162146981

Gene position: 1184012-1182156 (Counterclockwise)

Preceding gene: 162146982

Following gene: 162146980

Centisome position: 30.02

GC content: 72.75

Gene sequence:

>1857_bases
ATGAAGACGACTGACGCCGCCGGCCCCTCGTCCGCGCCGCGCCGGCGGGCTGCCGGCCGGCGGGGAACGCGGGCGGAACA
CCGGCTGTCCGGCGATGCCACGGTGCGCGGCATCGCCATCGGCCCGGCGGCGGTGGCGCTGGAAAGCCCGGCGCCGGATA
TGGATCCCGACGCCCGCGCCGCCGACCCCGCACCCGAGCTGGAGCGCCTGGCCGAGGCCGTGGAACGGTCGGTGCGGCAG
GTCGAACGCCTGCGCGACCGCCTGGCGGTGCTGCCCGAGGACAGCCAGATCGAAATCGGATCGCTGCTGGAGGTCTATCG
CCGGATGCTCGGCCCGTCGCGCCTGCAGCGCGGCATCCGCCGCCGCATCGTCCAGGACGGACTGACAGCCGAAGCCGCCG
TCCGGCAGGAAACCGAAAGCCTGGCCCTGGCCCTGCTGGGCGGGGCCGACGCCCCGGTGCCCGAGGGCGAGGACGCCGCC
GCCGCCCAGCGCCGCGCGGGCGAATTCCGCGAAATCGGCCGGCGGCTGCTGCGCAACCTGGGGCGCATGCCCTTTCGCTC
GTTCAGCGCGCTTCCCGAGGGCGCGGTGCTGGTCACCGAACAGTTGCGCCCCGCCGATGCGGCGCTGATCGACCCGTCGC
GCATCGTCGCGGTCGCGACCGAGGAAGGGGGCGCCACCGACCACACCGCCATCATGCTGCGCGCCCTGGGCATTCCCGCC
GTGCTGGCCGCCCACGGGCTGATGGCGCGGGTGCGCGAGGGGGCCACCGTGGTGGTGGACGGCACCGCCGGGCTGGTGGT
GGTGGACCCGACCGAGGATACGCTGGCGGCGGCGCGCGGCGGGGTGGCCGAACATGCGCGCGAACGCCAGGCGCTGGGCC
GGCTGCGCCGCCTGCCGGCCCGCCTGTCCAGCGGCGAGAAGCTGCATCTGCAGGCCAATCTGGAACTGCCGGCCGAACTG
GCGCTGATCGCGCAGTCCGGCGCGTCGGGCATCGGCCTGCTGCGCACGGAATTCCTGTTCATCAATGCCGAAACCATGCC
GGACGAGGACAGCCAGGCCGCGATCTATTCCGAAATCATCACCGCGATGGCAGGGGATACCACCACCATCCGCGTGGTGG
ACTGGGGCGGCGAAAAGCATAGCGAGGCCCTGAACCGCGCGGGGCTGGACCGTGACGGCGACAACGTCAATCCGGCGCTG
GGCGTGCGCGGCCTGCGGCTGCTGCTGCGCCATCCCGCGATCCTGGAAACCCAGTTCGCCGCGATCCTGAAGGCGTCGTC
CGCCGGGCCGATGCGCGTCATGCTGCCGATGGTCACGACCGTCCCGGAACTGCGCGAGGCCCGCGACATCTATCAGCGCG
TCGCGCGCCGCCTGCGCCGCCGGGGGGTGAAGCTGGGTGACAGCCTGCCGCCGCTGGGCATCATGGTCGAAACCCCGGCC
GCCGCCATCATGGGCGATGCGCTGGCGCAGGAAGCCGAATTCCTGGCCATCGGCACCAACGACCTGACGATGTACACGCT
GGCGGCCGATCGCGCCCTGGCCGATGTCGCGTCCCTCTACCAGCCGCTGCATCCCGCCGTGCTGCGCCTGATCCAGACGG
TGACCGAGGCCGCGCTGCGCCAGTATCGTCCGATTTCGATCTGCGGGGAAATCGCCGGCGATCCGCGGGTGGTGCCGCTG
CTGGTCGGGCTGGGCCTGCGCAGCTTCTCGATGACCGCCTCCGCCGTGCCCCGGGTGAAGCGCAGGGTGCGCGCCCTGTC
GTTCGAGGACTGCCGACGGCTGGCCCACCGCGTGATGGAATCCCCGGACGTGGCCGAAGTCCTGTCCCTGATCGACGCCT
TCGCCGCGGGGGGGTAG

Upstream 100 bases:

>100_bases
CTGGGGGCCGGTCGGGGGGAATCCATTACCCTGATGGCCACGGGATGGGATGCCCATGCGGCGATCGAGGCCCTGGTGGC
CCTGATCGAGGCGGGCTTCC

Downstream 100 bases:

>100_bases
CGGTCATGGATGGTCCGGTTGGCATCGGGGACCTTCACGCCGTCGAACTGCCGTGGCGGGACCCTGATGACGTGCTGTGG
GCGTGGCGGGACGAACCGTG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKTTDAAGPSSAPRRRAAGRRGTRAEHRLSGDATVRGIAIGPAAVALESPAPDMDPDARAADPAPELERLAEAVERSVRQ
VERLRDRLAVLPEDSQIEIGSLLEVYRRMLGPSRLQRGIRRRIVQDGLTAEAAVRQETESLALALLGGADAPVPEGEDAA
AAQRRAGEFREIGRRLLRNLGRMPFRSFSALPEGAVLVTEQLRPADAALIDPSRIVAVATEEGGATDHTAIMLRALGIPA
VLAAHGLMARVREGATVVVDGTAGLVVVDPTEDTLAAARGGVAEHARERQALGRLRRLPARLSSGEKLHLQANLELPAEL
ALIAQSGASGIGLLRTEFLFINAETMPDEDSQAAIYSEIITAMAGDTTTIRVVDWGGEKHSEALNRAGLDRDGDNVNPAL
GVRGLRLLLRHPAILETQFAAILKASSAGPMRVMLPMVTTVPELREARDIYQRVARRLRRRGVKLGDSLPPLGIMVETPA
AAIMGDALAQEAEFLAIGTNDLTMYTLAADRALADVASLYQPLHPAVLRLIQTVTEAALRQYRPISICGEIAGDPRVVPL
LVGLGLRSFSMTASAVPRVKRRVRALSFEDCRRLAHRVMESPDVAEVLSLIDAFAAGG

Sequences:

>Translated_618_residues
MKTTDAAGPSSAPRRRAAGRRGTRAEHRLSGDATVRGIAIGPAAVALESPAPDMDPDARAADPAPELERLAEAVERSVRQ
VERLRDRLAVLPEDSQIEIGSLLEVYRRMLGPSRLQRGIRRRIVQDGLTAEAAVRQETESLALALLGGADAPVPEGEDAA
AAQRRAGEFREIGRRLLRNLGRMPFRSFSALPEGAVLVTEQLRPADAALIDPSRIVAVATEEGGATDHTAIMLRALGIPA
VLAAHGLMARVREGATVVVDGTAGLVVVDPTEDTLAAARGGVAEHARERQALGRLRRLPARLSSGEKLHLQANLELPAEL
ALIAQSGASGIGLLRTEFLFINAETMPDEDSQAAIYSEIITAMAGDTTTIRVVDWGGEKHSEALNRAGLDRDGDNVNPAL
GVRGLRLLLRHPAILETQFAAILKASSAGPMRVMLPMVTTVPELREARDIYQRVARRLRRRGVKLGDSLPPLGIMVETPA
AAIMGDALAQEAEFLAIGTNDLTMYTLAADRALADVASLYQPLHPAVLRLIQTVTEAALRQYRPISICGEIAGDPRVVPL
LVGLGLRSFSMTASAVPRVKRRVRALSFEDCRRLAHRVMESPDVAEVLSLIDAFAAGG
>Mature_618_residues
MKTTDAAGPSSAPRRRAAGRRGTRAEHRLSGDATVRGIAIGPAAVALESPAPDMDPDARAADPAPELERLAEAVERSVRQ
VERLRDRLAVLPEDSQIEIGSLLEVYRRMLGPSRLQRGIRRRIVQDGLTAEAAVRQETESLALALLGGADAPVPEGEDAA
AAQRRAGEFREIGRRLLRNLGRMPFRSFSALPEGAVLVTEQLRPADAALIDPSRIVAVATEEGGATDHTAIMLRALGIPA
VLAAHGLMARVREGATVVVDGTAGLVVVDPTEDTLAAARGGVAEHARERQALGRLRRLPARLSSGEKLHLQANLELPAEL
ALIAQSGASGIGLLRTEFLFINAETMPDEDSQAAIYSEIITAMAGDTTTIRVVDWGGEKHSEALNRAGLDRDGDNVNPAL
GVRGLRLLLRHPAILETQFAAILKASSAGPMRVMLPMVTTVPELREARDIYQRVARRLRRRGVKLGDSLPPLGIMVETPA
AAIMGDALAQEAEFLAIGTNDLTMYTLAADRALADVASLYQPLHPAVLRLIQTVTEAALRQYRPISICGEIAGDPRVVPL
LVGLGLRSFSMTASAVPRVKRRVRALSFEDCRRLAHRVMESPDVAEVLSLIDAFAAGG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=487, Percent_Identity=35.3182751540041, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1789193, Length=585, Percent_Identity=33.5042735042735, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI48994992, Length=528, Percent_Identity=29.9242424242424, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788726, Length=556, Percent_Identity=29.8561151079137, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1787994, Length=452, Percent_Identity=24.5575221238938, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 66100; Mature: 66100

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTDAAGPSSAPRRRAAGRRGTRAEHRLSGDATVRGIAIGPAAVALESPAPDMDPDARA
CCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCEEEEEEECCHHHEECCCCCCCCCCCCC
ADPAPELERLAEAVERSVRQVERLRDRLAVLPEDSQIEIGSLLEVYRRMLGPSRLQRGIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
RRIVQDGLTAEAAVRQETESLALALLGGADAPVPEGEDAAAAQRRAGEFREIGRRLLRNL
HHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GRMPFRSFSALPEGAVLVTEQLRPADAALIDPSRIVAVATEEGGATDHTAIMLRALGIPA
CCCCCHHHHCCCCCCEEEECCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHCCHH
VLAAHGLMARVREGATVVVDGTAGLVVVDPTEDTLAAARGGVAEHARERQALGRLRRLPA
HHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RLSSGEKLHLQANLELPAELALIAQSGASGIGLLRTEFLFINAETMPDEDSQAAIYSEII
HCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
TAMAGDTTTIRVVDWGGEKHSEALNRAGLDRDGDNVNPALGVRGLRLLLRHPAILETQFA
HHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AILKASSAGPMRVMLPMVTTVPELREARDIYQRVARRLRRRGVKLGDSLPPLGIMVETPA
HHHHCCCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCH
AAIMGDALAQEAEFLAIGTNDLTMYTLAADRALADVASLYQPLHPAVLRLIQTVTEAALR
HHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QYRPISICGEIAGDPRVVPLLVGLGLRSFSMTASAVPRVKRRVRALSFEDCRRLAHRVME
HCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
SPDVAEVLSLIDAFAAGG
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTTDAAGPSSAPRRRAAGRRGTRAEHRLSGDATVRGIAIGPAAVALESPAPDMDPDARA
CCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCEEEEEEECCHHHEECCCCCCCCCCCCC
ADPAPELERLAEAVERSVRQVERLRDRLAVLPEDSQIEIGSLLEVYRRMLGPSRLQRGIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
RRIVQDGLTAEAAVRQETESLALALLGGADAPVPEGEDAAAAQRRAGEFREIGRRLLRNL
HHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GRMPFRSFSALPEGAVLVTEQLRPADAALIDPSRIVAVATEEGGATDHTAIMLRALGIPA
CCCCCHHHHCCCCCCEEEECCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHCCHH
VLAAHGLMARVREGATVVVDGTAGLVVVDPTEDTLAAARGGVAEHARERQALGRLRRLPA
HHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RLSSGEKLHLQANLELPAELALIAQSGASGIGLLRTEFLFINAETMPDEDSQAAIYSEII
HCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHH
TAMAGDTTTIRVVDWGGEKHSEALNRAGLDRDGDNVNPALGVRGLRLLLRHPAILETQFA
HHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
AILKASSAGPMRVMLPMVTTVPELREARDIYQRVARRLRRRGVKLGDSLPPLGIMVETPA
HHHHCCCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCH
AAIMGDALAQEAEFLAIGTNDLTMYTLAADRALADVASLYQPLHPAVLRLIQTVTEAALR
HHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QYRPISICGEIAGDPRVVPLLVGLGLRSFSMTASAVPRVKRRVRALSFEDCRRLAHRVME
HCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
SPDVAEVLSLIDAFAAGG
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]