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The map label for this gene is lutR [H]

Identifier: 162146820

GI number: 162146820

Start: 1017913

End: 1018671

Strand: Reverse

Name: lutR [H]

Synonym: GDI_1002

Alternate gene names: 162146820

Gene position: 1018671-1017913 (Counterclockwise)

Preceding gene: 162146823

Following gene: 162146818

Centisome position: 25.83

GC content: 64.16

Gene sequence:

>759_bases
ATGACTGCTCCGATCTCCCTGTCACGCCTGCCGCGTCCCTCCCTGGTCGATGCGGCGCTTGCCGCCATGCGTGAGCGGAT
CACATCCGGTGACTGGCCCGTTGGCCAGCGCATTCCCAAGGAGACAGAACTGGCCGAAATGCTCCAGATCGGCCGCAACA
CCGTGCGCGAGGCCCTGCGCGTGTTGTCTCATGCCGGCATGATCGAGGTGCGTCAGGGCGACGGGACCTACGTGCGTGCC
TTGCATGAACCCGCAACGATTATGGCGCAGGTCAGTCATACCAGCCTGCGCGATCATTTCGAGTTGCGTGTGATGCTGGA
GACGGAATCCGCGCGTCTGGCAGCGCTCCGCCGGACAACGCGGGACATCCGCAGAATGGGGGCAGCCCTGAAGGCGCGTG
GCGAACGGAGTTCCTACAGCGACCTTGCCCGCTTTCTGGACCTGGATGCTTCCTTCCATCTGGCCGTCGCGGAGGCCAGT
CACAACGATGCGCTGATCCCTCTTTATCGCTATTTTCTGGATGCGGCGCGCGAAGCCGCTCTTTCTGCCCTCGTTGAGCA
TGGAGTCACCGAGCCGAAGCTGGAGCTTCACGAACGGCTGTTCCAGGCGATCGAAGCGGGGGATGCACTGCGGGCAGTGA
GGGCGGCGCGGGCTATCCTTCAGCCATTGATCGCGGCCTTTACCGACGGCAAGTCTCCGGGCACGGCCGCGGCTCGCGGC
GTTCGCAAGCGGGCGGCACAGGAGCGTCATAAAGCCTGA

Upstream 100 bases:

>100_bases
TCAGTATGCCGCCGATCCTATCGTGCAAAGGTCGTATGCCGGCCTGCCATAATGACATTCAGACATGGGATGATCCGCTC
AAACAGGCTATGCTGCGCAC

Downstream 100 bases:

>100_bases
GCGGTCTCTGGCTTTATGATCGGGGCGCGAGGGCTCATGACACGTAGGACCGTGCGTCGCAGCCATTGATGGACCTGGTC
AGTGTCGTGTCGGGGATGCC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTAPISLSRLPRPSLVDAALAAMRERITSGDWPVGQRIPKETELAEMLQIGRNTVREALRVLSHAGMIEVRQGDGTYVRA
LHEPATIMAQVSHTSLRDHFELRVMLETESARLAALRRTTRDIRRMGAALKARGERSSYSDLARFLDLDASFHLAVAEAS
HNDALIPLYRYFLDAAREAALSALVEHGVTEPKLELHERLFQAIEAGDALRAVRAARAILQPLIAAFTDGKSPGTAAARG
VRKRAAQERHKA

Sequences:

>Translated_252_residues
MTAPISLSRLPRPSLVDAALAAMRERITSGDWPVGQRIPKETELAEMLQIGRNTVREALRVLSHAGMIEVRQGDGTYVRA
LHEPATIMAQVSHTSLRDHFELRVMLETESARLAALRRTTRDIRRMGAALKARGERSSYSDLARFLDLDASFHLAVAEAS
HNDALIPLYRYFLDAAREAALSALVEHGVTEPKLELHERLFQAIEAGDALRAVRAARAILQPLIAAFTDGKSPGTAAARG
VRKRAAQERHKA
>Mature_251_residues
TAPISLSRLPRPSLVDAALAAMRERITSGDWPVGQRIPKETELAEMLQIGRNTVREALRVLSHAGMIEVRQGDGTYVRAL
HEPATIMAQVSHTSLRDHFELRVMLETESARLAALRRTTRDIRRMGAALKARGERSSYSDLARFLDLDASFHLAVAEASH
NDALIPLYRYFLDAAREAALSALVEHGVTEPKLELHERLFQAIEAGDALRAVRAARAILQPLIAAFTDGKSPGTAAARGV
RKRAAQERHKA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=156, Percent_Identity=37.1794871794872, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790032, Length=225, Percent_Identity=32.4444444444444, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789353, Length=148, Percent_Identity=36.4864864864865, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082208, Length=151, Percent_Identity=33.7748344370861, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786303, Length=180, Percent_Identity=30, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27781; Mature: 27650

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPISLSRLPRPSLVDAALAAMRERITSGDWPVGQRIPKETELAEMLQIGRNTVREALR
CCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLSHAGMIEVRQGDGTYVRALHEPATIMAQVSHTSLRDHFELRVMLETESARLAALRRTT
HHHHCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHH
RDIRRMGAALKARGERSSYSDLARFLDLDASFHLAVAEASHNDALIPLYRYFLDAAREAA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LSALVEHGVTEPKLELHERLFQAIEAGDALRAVRAARAILQPLIAAFTDGKSPGTAAARG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VRKRAAQERHKA
HHHHHHHHHCCC
>Mature Secondary Structure 
TAPISLSRLPRPSLVDAALAAMRERITSGDWPVGQRIPKETELAEMLQIGRNTVREALR
CCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLSHAGMIEVRQGDGTYVRALHEPATIMAQVSHTSLRDHFELRVMLETESARLAALRRTT
HHHHCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHH
RDIRRMGAALKARGERSSYSDLARFLDLDASFHLAVAEASHNDALIPLYRYFLDAAREAA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LSALVEHGVTEPKLELHERLFQAIEAGDALRAVRAARAILQPLIAAFTDGKSPGTAAARG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VRKRAAQERHKA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]