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The map label for this gene is metB [H]

Identifier: 162146602

GI number: 162146602

Start: 795616

End: 796866

Strand: Reverse

Name: metB [H]

Synonym: GDI_0780

Alternate gene names: 162146602

Gene position: 796866-795616 (Counterclockwise)

Preceding gene: 162146603

Following gene: 162146600

Centisome position: 20.2

GC content: 68.11

Gene sequence:

>1251_bases
ATGCCCCGAGGCCCGGCCTTTCTGACGATTCTGTTCCGGGAGCTCATGATGAGCAACGATTCGACCGACCGAGCCTACCG
CCCCGCGACCCGCCTGCTGCATTCCGGCGTCGAGCGCACCCCGTTCGGCGAGACGAGCGAGGCGATGTTCCTGACATCGG
GCTTCGTCTACGACAATGCCGAACAGGCCGAGGCGACCTTCACCGGCGACGTGACGCATTATCAGTACAGCCGGTTCGGC
AACCCCACCGTCGAGGCGCTGGAGAAGCGCCTGGCGGACCTGGAAGGGGCCGAGGCCTGCATCGCGACCTCGACGGGCAT
GGGGGCGGTGTCGTCCGCGCTGCTGTCGCACGTCAAGGCCGGGGACCGGGTGGTGGCGTCGCGCGCGCTGTTCGGGTCGT
GCCACTGGATCGTCGCCAACCTGCTGCCGCGCTACGGGGTGGAAACGGTGTTCGTGGACGGGGGCGACATGGACGCGTGG
GCGGAAGCCCTGGCGCGGCCCACGGCGGCGGTCCTGCTGGAAAGCCCGTCGAACCCGATGCTGGACATCCTGGACATCCG
CGCCATTTCCGACCTGGCGCACTGGGCCGGAGCCCTGGTGGTGGTCGACAATGTTTTCGCGACCCCGCTGCTGCAGAAGC
CGCTGGAACTGGGGGCGGATGTCGTCGTGTATTCCTGCACCAAGCATATCGACGGCCAGGGGCGCGTCCTGGGCGGCGCG
GTGCTGGGCCGCAAGGACTGGATCACCGATACCCTGCAGCCCTTCACCCCGCATACCGGCGACGCCCTGTCGCCGTTCAA
TGCCTGGGTGATGCTGAAGGGGCTGGAGACGCTGGCCCTGCGCGTCCGGGCAATGACCGACAACGCCGCCGCCGTCGCCG
ATCACCTGGCCGGCGCCGAGGGGGTGACGCGGGTGTTCTATCCCGACCGGCCCGACCATCCGCAATACGCGCTGGCGCAG
GCGCAGATGAGCGGCGCCTCCACCCTGGTCGCCTTCGAGGTCGCGGGCGGCAAGGCGGGCGCCTTCGCCTTCATGAACGC
GTTGCGGCTGATCGCGATTTCCAACAATCTGGGTGATGCGCGATCGATGGTGACGCACCCGGCCACCACCACGCACATGA
AGATCGGCGCCGAGGAACGGGCACGACTGGGCATCACCGACGGCGTGATCCGCTTTTCGGTGGGTCTGGAAGACAGCGCC
GATCTGAAGGATGATCTGGATCGCGGGCTGGCCGCCCTTCGGTCACGCTGA

Upstream 100 bases:

>100_bases
CTAAAGAGGCCTGACTTCCGCCAGAATCCGCGGGCGTCACGGTTTCCACGGTCGGATTTTCGCGTCGGGACGCGGGGCCG
CCGGATCGGTTCGACACCGG

Downstream 100 bases:

>100_bases
CGCAGGCAGGAACGCGCCATGGCCCAAGACAGACCGCCGCCCCCCGGACTGGACCCCGACCCGGACGCCGCCCTGGCCGA
GGCCATGGACGGCGCCCCGT

Product: cystathionine gamma-synthase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPRGPAFLTILFRELMMSNDSTDRAYRPATRLLHSGVERTPFGETSEAMFLTSGFVYDNAEQAEATFTGDVTHYQYSRFG
NPTVEALEKRLADLEGAEACIATSTGMGAVSSALLSHVKAGDRVVASRALFGSCHWIVANLLPRYGVETVFVDGGDMDAW
AEALARPTAAVLLESPSNPMLDILDIRAISDLAHWAGALVVVDNVFATPLLQKPLELGADVVVYSCTKHIDGQGRVLGGA
VLGRKDWITDTLQPFTPHTGDALSPFNAWVMLKGLETLALRVRAMTDNAAAVADHLAGAEGVTRVFYPDRPDHPQYALAQ
AQMSGASTLVAFEVAGGKAGAFAFMNALRLIAISNNLGDARSMVTHPATTTHMKIGAEERARLGITDGVIRFSVGLEDSA
DLKDDLDRGLAALRSR

Sequences:

>Translated_416_residues
MPRGPAFLTILFRELMMSNDSTDRAYRPATRLLHSGVERTPFGETSEAMFLTSGFVYDNAEQAEATFTGDVTHYQYSRFG
NPTVEALEKRLADLEGAEACIATSTGMGAVSSALLSHVKAGDRVVASRALFGSCHWIVANLLPRYGVETVFVDGGDMDAW
AEALARPTAAVLLESPSNPMLDILDIRAISDLAHWAGALVVVDNVFATPLLQKPLELGADVVVYSCTKHIDGQGRVLGGA
VLGRKDWITDTLQPFTPHTGDALSPFNAWVMLKGLETLALRVRAMTDNAAAVADHLAGAEGVTRVFYPDRPDHPQYALAQ
AQMSGASTLVAFEVAGGKAGAFAFMNALRLIAISNNLGDARSMVTHPATTTHMKIGAEERARLGITDGVIRFSVGLEDSA
DLKDDLDRGLAALRSR
>Mature_415_residues
PRGPAFLTILFRELMMSNDSTDRAYRPATRLLHSGVERTPFGETSEAMFLTSGFVYDNAEQAEATFTGDVTHYQYSRFGN
PTVEALEKRLADLEGAEACIATSTGMGAVSSALLSHVKAGDRVVASRALFGSCHWIVANLLPRYGVETVFVDGGDMDAWA
EALARPTAAVLLESPSNPMLDILDIRAISDLAHWAGALVVVDNVFATPLLQKPLELGADVVVYSCTKHIDGQGRVLGGAV
LGRKDWITDTLQPFTPHTGDALSPFNAWVMLKGLETLALRVRAMTDNAAAVADHLAGAEGVTRVFYPDRPDHPQYALAQA
QMSGASTLVAFEVAGGKAGAFAFMNALRLIAISNNLGDARSMVTHPATTTHMKIGAEERARLGITDGVIRFSVGLEDSAD
LKDDLDRGLAALRSR

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=387, Percent_Identity=31.5245478036176, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=30.3108808290155, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=28.7564766839378, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1790375, Length=349, Percent_Identity=35.8166189111748, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=25.1269035532995, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=37.9518072289157, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=350, Percent_Identity=38, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=347, Percent_Identity=32.2766570605187, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=389, Percent_Identity=32.9048843187661, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=28.5365853658537, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=26.4705882352941, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=294, Percent_Identity=27.5510204081633, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=344, Percent_Identity=26.1627906976744, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=34.5360824742268, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 44547; Mature: 44416

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGPAFLTILFRELMMSNDSTDRAYRPATRLLHSGVERTPFGETSEAMFLTSGFVYDNA
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCC
EQAEATFTGDVTHYQYSRFGNPTVEALEKRLADLEGAEACIATSTGMGAVSSALLSHVKA
CCCCEEEECCCCEEEHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCC
GDRVVASRALFGSCHWIVANLLPRYGVETVFVDGGDMDAWAEALARPTAAVLLESPSNPM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCC
LDILDIRAISDLAHWAGALVVVDNVFATPLLQKPLELGADVVVYSCTKHIDGQGRVLGGA
CHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEHE
VLGRKDWITDTLQPFTPHTGDALSPFNAWVMLKGLETLALRVRAMTDNAAAVADHLAGAE
EECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
GVTRVFYPDRPDHPQYALAQAQMSGASTLVAFEVAGGKAGAFAFMNALRLIAISNNLGDA
CCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEECCCCHH
RSMVTHPATTTHMKIGAEERARLGITDGVIRFSVGLEDSADLKDDLDRGLAALRSR
HHHHCCCCCCEEEEECCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRGPAFLTILFRELMMSNDSTDRAYRPATRLLHSGVERTPFGETSEAMFLTSGFVYDNA
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCC
EQAEATFTGDVTHYQYSRFGNPTVEALEKRLADLEGAEACIATSTGMGAVSSALLSHVKA
CCCCEEEECCCCEEEHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCC
GDRVVASRALFGSCHWIVANLLPRYGVETVFVDGGDMDAWAEALARPTAAVLLESPSNPM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCC
LDILDIRAISDLAHWAGALVVVDNVFATPLLQKPLELGADVVVYSCTKHIDGQGRVLGGA
CHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEHE
VLGRKDWITDTLQPFTPHTGDALSPFNAWVMLKGLETLALRVRAMTDNAAAVADHLAGAE
EECCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
GVTRVFYPDRPDHPQYALAQAQMSGASTLVAFEVAGGKAGAFAFMNALRLIAISNNLGDA
CCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEECCCCHH
RSMVTHPATTTHMKIGAEERARLGITDGVIRFSVGLEDSADLKDDLDRGLAALRSR
HHHHCCCCCCEEEEECCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]