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The map label for this gene is folE [H]

Identifier: 162146600

GI number: 162146600

Start: 794466

End: 795083

Strand: Reverse

Name: folE [H]

Synonym: GDI_0778

Alternate gene names: 162146600

Gene position: 795083-794466 (Counterclockwise)

Preceding gene: 162146602

Following gene: 162146596

Centisome position: 20.16

GC content: 64.72

Gene sequence:

>618_bases
ATGACCCCCACCGACACGGTTCCCCTGCCCCGTCCCTCTCGCGAGGAGGCGGAAGACGCGATCCGCACCCTGCTACGCTG
GGCGGGCGAAGATCCCGCCCGCGAAGGGCTGCTCGATACGCCGGGCCGCGTAGTGCGGTCGTACGAGGAATTCTTCCAGG
GCTATGCCGAGGACCCGTCCGAAATCCTGTCGCGCACCTTCTCGGAAGTCGATGACTACGACGAGATCGTGCTGCTGCGC
GACATCCGTTTCGAAAGCCATTGCGAGCACCACATGGTGCCGATCATCGGCGTCGCGCATGTCGCCTACCTGCCCGGCAA
GCGGGTGGTGGGGATTTCGAAACTGGCGCGGGTGGTCGAGGCCTTTTCCCGCCGGCTGCAGATCCAGGAACGCCTGACGG
CGCAGATCGCCAATACGATCAACGATGTGCTGCAACCGCATGGCGTCGCGGTGATCCTGGAGGCCGGGCACCAATGCATG
ACCACGCGCGGGGTGCATCGGCCCGGCGTATCGATGGTGACCAGCCGCATGCTGGGGGTATTCCGCACCAATTCGGATAC
GCGGCGCGAACTGATGGCGATGCTGAACCGGCCTTCGGCCGCCACCACCCATATATAA

Upstream 100 bases:

>100_bases
TTCGACGTGCGGATTCCGCCGTTCAGCCTGGATTGCCCGTACCACCCCGCCATCATTCATTAAGGGCGCCGCTCCGGCAC
GCCCCCAGCAAGACAGGCTC

Downstream 100 bases:

>100_bases
CCGCCCGTTCGACCGGGTGGGTGGTCCCCGCCGGTGGCGGGGACCTTCGGGTCAGTCGCCCGCCTTTTCGTTGATCTGAT
AGGTCAGGGTCACGTGGCTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTPTDTVPLPRPSREEAEDAIRTLLRWAGEDPAREGLLDTPGRVVRSYEEFFQGYAEDPSEILSRTFSEVDDYDEIVLLR
DIRFESHCEHHMVPIIGVAHVAYLPGKRVVGISKLARVVEAFSRRLQIQERLTAQIANTINDVLQPHGVAVILEAGHQCM
TTRGVHRPGVSMVTSRMLGVFRTNSDTRRELMAMLNRPSAATTHI

Sequences:

>Translated_205_residues
MTPTDTVPLPRPSREEAEDAIRTLLRWAGEDPAREGLLDTPGRVVRSYEEFFQGYAEDPSEILSRTFSEVDDYDEIVLLR
DIRFESHCEHHMVPIIGVAHVAYLPGKRVVGISKLARVVEAFSRRLQIQERLTAQIANTINDVLQPHGVAVILEAGHQCM
TTRGVHRPGVSMVTSRMLGVFRTNSDTRRELMAMLNRPSAATTHI
>Mature_204_residues
TPTDTVPLPRPSREEAEDAIRTLLRWAGEDPAREGLLDTPGRVVRSYEEFFQGYAEDPSEILSRTFSEVDDYDEIVLLRD
IRFESHCEHHMVPIIGVAHVAYLPGKRVVGISKLARVVEAFSRRLQIQERLTAQIANTINDVLQPHGVAVILEAGHQCMT
TRGVHRPGVSMVTSRMLGVFRTNSDTRRELMAMLNRPSAATTHI

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=201, Percent_Identity=45.273631840796, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI4503949, Length=201, Percent_Identity=45.273631840796, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI66932970, Length=171, Percent_Identity=43.2748538011696, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=49.2647058823529, Blast_Score=141, Evalue=4e-34,
Organism=Escherichia coli, GI1788476, Length=172, Percent_Identity=37.7906976744186, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=179, Percent_Identity=46.927374301676, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=48.8888888888889, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24656782, Length=194, Percent_Identity=44.3298969072165, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24656796, Length=194, Percent_Identity=44.3298969072165, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24656791, Length=194, Percent_Identity=44.3298969072165, Blast_Score=158, Evalue=2e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23055; Mature: 22923

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTDTVPLPRPSREEAEDAIRTLLRWAGEDPAREGLLDTPGRVVRSYEEFFQGYAEDPS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
EILSRTFSEVDDYDEIVLLRDIRFESHCEHHMVPIIGVAHVAYLPGKRVVGISKLARVVE
HHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AFSRRLQIQERLTAQIANTINDVLQPHGVAVILEAGHQCMTTRGVHRPGVSMVTSRMLGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHH
FRTNSDTRRELMAMLNRPSAATTHI
HHCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPTDTVPLPRPSREEAEDAIRTLLRWAGEDPAREGLLDTPGRVVRSYEEFFQGYAEDPS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
EILSRTFSEVDDYDEIVLLRDIRFESHCEHHMVPIIGVAHVAYLPGKRVVGISKLARVVE
HHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AFSRRLQIQERLTAQIANTINDVLQPHGVAVILEAGHQCMTTRGVHRPGVSMVTSRMLGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHH
FRTNSDTRRELMAMLNRPSAATTHI
HHCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]