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The map label for this gene is glcD [H]

Identifier: 162146567

GI number: 162146567

Start: 761645

End: 763141

Strand: Direct

Name: glcD [H]

Synonym: GDI_0744

Alternate gene names: 162146567

Gene position: 761645-763141 (Clockwise)

Preceding gene: 162146563

Following gene: 162146568

Centisome position: 19.31

GC content: 67.8

Gene sequence:

>1497_bases
ATGCGCATGCCCGATCCCGATCCGGCGATCATCGCCCGTCGCGCGGAACTGGCCGGGATCGATCTGCGCCGGATCGTGGG
CCCCGAATGGGTGCTGGATGACGAGGCCGAGCGCCGCCCCTATCGCTGCGACGGGATCACCGCGTACCAGGCGACGCCGC
TGCTGGTCGTGCTGCCGGGCAGCACGCGGGAAATCCAGGACGTGCTGCGGTTCTGCCTGGCCGAGGGCGTGAAGATCGTG
CCGCGCGGATCGGGTACCTCGCTGTCCGGGGGCGCGCTGCCGCTGGAAGACGGCATCCTGCTGGTGACGTCGCGCCTGTG
CGCGGTCCTGTCGGTCGATGCCGTAAACCGCGTGGCCCGCGTGCAGCCGGGGGTGGCGAACGTCCGGATTTCCGATTCCG
TGCGCCATCTCGGCCTGTGCTACATGCCCGACCCGTCCAGCCAGATGATTTCGACCATCGGCGGCAATGTCGCGGAAAAT
TCCGGCGGCGTGCACTGCCTGAAATATGGCGTGACCGCCAACCACATCGTGGGACTGGAAGCCGTGCTGGTCTCGGGCGA
GATCGTACGCCTGGGGGGCGCCTTCCAGGGCGCCGCGGATGGCGAACTGGACCTGATCGGCCTGCTGACGGGGTCCGAGG
GCCTGCTGGCGGTCATCACCGAAATTTCGGTGCGGCTGGTGCCGGTGCCCGAGACCGCGCGCGTGGTGTCGATGGAATTC
GCCCATGTCGAGGATGCCTGCCGCTGCGTGGGCGCCATCATCGGCGCCGGCATCATTCCCGCCGGGCTGGAATTCATGGA
CCGCAAGGTGGTGAACGCGGTGTCCGCCCTGCTGCGCGAAACCTGTGATCCCCGCACCGACGCCCTGCTGCTGTGCGAAC
TCGACGGAACGGCGGCCGAGGTCGATGCCCTGATCGAGACCGTGGCCGACCTGGCGCGACGCGGTGGCGCCATCGCCGTG
CGCGTCAGCAGCACCGACGAGGAACGGGAACGGATCTGGCGCAGCCGCAAACTGGCATTTTCCGCAATCGGCAAGCTGTG
CAAGGATTATTACTGTACCGACGGCACCATCCCCCGCAACCGGTTGCCGGACATCCTGGCGCGTATGGCGATCCTGTCGC
GCACCTACGGGCTCGACTACGCCAACTGCTTCCATGCCGGTGACGGCAACCTGCACCCGATCATCATGTACGACGCCGCC
CGCCCCGGCGACCTGGAGAAGGCCGAGGCCTTCGGCGCCGACGTGCTGCGCGCCTGCGTGGACATGGGCGGGGTGCTGAG
CGGCGAGCACGGGGTGGGCGTCGAAAAGCGCGACCTGATGACCCATCAGTTCGCCCCCGCCGACCTGCGGCAGCAGCAGG
TTCTGAAATGCGCCTTCGATCCCGGCGAACTTCTCAATCCCGGCAAGGTGTTTCCCACCCCCTGCCGCTGCGCCGAATTC
GGGCGGATGCATGTACGGGGGGGCGCGCTGCGCTTCCCCCATATACCGAGGTTTTGA

Upstream 100 bases:

>100_bases
CCGACGGGCACGTTATTACATAAGCCAGCGGCGACTCAAGCCATGGGACAATGGCGGTGCCTCCCTGCCGGGGGTGGGGT
GTCGCGGGGGGAACAGACCA

Downstream 100 bases:

>100_bases
TGAGCATCCTGGCCGACCCCATCGTCTACCGCCCCACCCGGGCCCCCGACGTTGCCGAAATGGTGCGGGCCGCCTGCTCC
AAGGGGCAGGCGCTGGCGAT

Product: glycolate oxidase subunit glcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MRMPDPDPAIIARRAELAGIDLRRIVGPEWVLDDEAERRPYRCDGITAYQATPLLVVLPGSTREIQDVLRFCLAEGVKIV
PRGSGTSLSGGALPLEDGILLVTSRLCAVLSVDAVNRVARVQPGVANVRISDSVRHLGLCYMPDPSSQMISTIGGNVAEN
SGGVHCLKYGVTANHIVGLEAVLVSGEIVRLGGAFQGAADGELDLIGLLTGSEGLLAVITEISVRLVPVPETARVVSMEF
AHVEDACRCVGAIIGAGIIPAGLEFMDRKVVNAVSALLRETCDPRTDALLLCELDGTAAEVDALIETVADLARRGGAIAV
RVSSTDEERERIWRSRKLAFSAIGKLCKDYYCTDGTIPRNRLPDILARMAILSRTYGLDYANCFHAGDGNLHPIIMYDAA
RPGDLEKAEAFGADVLRACVDMGGVLSGEHGVGVEKRDLMTHQFAPADLRQQQVLKCAFDPGELLNPGKVFPTPCRCAEF
GRMHVRGGALRFPHIPRF

Sequences:

>Translated_498_residues
MRMPDPDPAIIARRAELAGIDLRRIVGPEWVLDDEAERRPYRCDGITAYQATPLLVVLPGSTREIQDVLRFCLAEGVKIV
PRGSGTSLSGGALPLEDGILLVTSRLCAVLSVDAVNRVARVQPGVANVRISDSVRHLGLCYMPDPSSQMISTIGGNVAEN
SGGVHCLKYGVTANHIVGLEAVLVSGEIVRLGGAFQGAADGELDLIGLLTGSEGLLAVITEISVRLVPVPETARVVSMEF
AHVEDACRCVGAIIGAGIIPAGLEFMDRKVVNAVSALLRETCDPRTDALLLCELDGTAAEVDALIETVADLARRGGAIAV
RVSSTDEERERIWRSRKLAFSAIGKLCKDYYCTDGTIPRNRLPDILARMAILSRTYGLDYANCFHAGDGNLHPIIMYDAA
RPGDLEKAEAFGADVLRACVDMGGVLSGEHGVGVEKRDLMTHQFAPADLRQQQVLKCAFDPGELLNPGKVFPTPCRCAEF
GRMHVRGGALRFPHIPRF
>Mature_498_residues
MRMPDPDPAIIARRAELAGIDLRRIVGPEWVLDDEAERRPYRCDGITAYQATPLLVVLPGSTREIQDVLRFCLAEGVKIV
PRGSGTSLSGGALPLEDGILLVTSRLCAVLSVDAVNRVARVQPGVANVRISDSVRHLGLCYMPDPSSQMISTIGGNVAEN
SGGVHCLKYGVTANHIVGLEAVLVSGEIVRLGGAFQGAADGELDLIGLLTGSEGLLAVITEISVRLVPVPETARVVSMEF
AHVEDACRCVGAIIGAGIIPAGLEFMDRKVVNAVSALLRETCDPRTDALLLCELDGTAAEVDALIETVADLARRGGAIAV
RVSSTDEERERIWRSRKLAFSAIGKLCKDYYCTDGTIPRNRLPDILARMAILSRTYGLDYANCFHAGDGNLHPIIMYDAA
RPGDLEKAEAFGADVLRACVDMGGVLSGEHGVGVEKRDLMTHQFAPADLRQQQVLKCAFDPGELLNPGKVFPTPCRCAEF
GRMHVRGGALRFPHIPRF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=28.4705882352941, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI37595754, Length=447, Percent_Identity=26.8456375838926, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI119964728, Length=474, Percent_Identity=29.1139240506329, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI4501993, Length=254, Percent_Identity=28.3464566929134, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=47.0941883767535, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1787977, Length=506, Percent_Identity=24.901185770751, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994907, Length=493, Percent_Identity=23.3265720081136, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=457, Percent_Identity=25.601750547046, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=457, Percent_Identity=24.507658643326, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=380, Percent_Identity=24.7368421052632, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17534361, Length=482, Percent_Identity=24.4813278008299, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17556096, Length=220, Percent_Identity=27.7272727272727, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=488, Percent_Identity=26.844262295082, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6320023, Length=454, Percent_Identity=27.3127753303965, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320764, Length=469, Percent_Identity=24.7334754797441, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18921117, Length=466, Percent_Identity=27.0386266094421, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24639277, Length=466, Percent_Identity=27.0386266094421, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24639275, Length=466, Percent_Identity=27.0386266094421, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24653753, Length=245, Percent_Identity=27.3469387755102, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53420; Mature: 53420

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMPDPDPAIIARRAELAGIDLRRIVGPEWVLDDEAERRPYRCDGITAYQATPLLVVLPG
CCCCCCCHHHHEEHHHHCCCCHHHHCCCCEEECCHHHCCCCCCCCEEEECCCCEEEEECC
STREIQDVLRFCLAEGVKIVPRGSGTSLSGGALPLEDGILLVTSRLCAVLSVDAVNRVAR
CCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQPGVANVRISDSVRHLGLCYMPDPSSQMISTIGGNVAENSGGVHCLKYGVTANHIVGLE
CCCCCEEEEECCCHHHCCEEECCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHE
AVLVSGEIVRLGGAFQGAADGELDLIGLLTGSEGLLAVITEISVRLVPVPETARVVSMEF
EEEECCCEEEECCCCCCCCCCCEEEEEEEECCCCHHHEEEECEEEEEECCCCCCEEEHHH
AHVEDACRCVGAIIGAGIIPAGLEFMDRKVVNAVSALLRETCDPRTDALLLCELDGTAAE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
VDALIETVADLARRGGAIAVRVSSTDEERERIWRSRKLAFSAIGKLCKDYYCTDGTIPRN
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RLPDILARMAILSRTYGLDYANCFHAGDGNLHPIIMYDAARPGDLEKAEAFGADVLRACV
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
DMGGVLSGEHGVGVEKRDLMTHQFAPADLRQQQVLKCAFDPGELLNPGKVFPTPCRCAEF
HHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHC
GRMHVRGGALRFPHIPRF
CCEEECCCEEECCCCCCC
>Mature Secondary Structure
MRMPDPDPAIIARRAELAGIDLRRIVGPEWVLDDEAERRPYRCDGITAYQATPLLVVLPG
CCCCCCCHHHHEEHHHHCCCCHHHHCCCCEEECCHHHCCCCCCCCEEEECCCCEEEEECC
STREIQDVLRFCLAEGVKIVPRGSGTSLSGGALPLEDGILLVTSRLCAVLSVDAVNRVAR
CCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VQPGVANVRISDSVRHLGLCYMPDPSSQMISTIGGNVAENSGGVHCLKYGVTANHIVGLE
CCCCCEEEEECCCHHHCCEEECCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHE
AVLVSGEIVRLGGAFQGAADGELDLIGLLTGSEGLLAVITEISVRLVPVPETARVVSMEF
EEEECCCEEEECCCCCCCCCCCEEEEEEEECCCCHHHEEEECEEEEEECCCCCCEEEHHH
AHVEDACRCVGAIIGAGIIPAGLEFMDRKVVNAVSALLRETCDPRTDALLLCELDGTAAE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
VDALIETVADLARRGGAIAVRVSSTDEERERIWRSRKLAFSAIGKLCKDYYCTDGTIPRN
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RLPDILARMAILSRTYGLDYANCFHAGDGNLHPIIMYDAARPGDLEKAEAFGADVLRACV
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
DMGGVLSGEHGVGVEKRDLMTHQFAPADLRQQQVLKCAFDPGELLNPGKVFPTPCRCAEF
HHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHC
GRMHVRGGALRFPHIPRF
CCEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]