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The map label for this gene is ispE

Identifier: 162146551

GI number: 162146551

Start: 747054

End: 747971

Strand: Direct

Name: ispE

Synonym: GDI_0728

Alternate gene names: 162146551

Gene position: 747054-747971 (Clockwise)

Preceding gene: 162146550

Following gene: 162146552

Centisome position: 18.94

GC content: 72.88

Gene sequence:

>918_bases
GTGACTGATCCGACAGGGACTGGAACGCTGCACGAATCCGCCCATGCGAAAATCAACCTCTACCTGCATGTAACGGGGCG
CCGGCCGGACGGATATCACCTGCTGGACAGCCTGGCGGTCTTCGCAGGGGCGGCGGACCGCCTGACCCTGCGTCCCGGTG
CGGCGGGCCAGGGGGAAGCGGTGGCCCTGGACATTGCCGGCGCGTTCGGCGCCGGGCTGGTGGCCGATACCGACAGCAAT
CTGGTGCTGCAGGCGGCGCGCAGGCTGCGGGCGGAAATGGGGGTCACCGACCGGCTGGCCCCCATGCGGATCGTGCTGGA
AAAATCACTGCCCGTCGCATCGGGAATCGGCGGCGGGTCGGCGGACGCGGCGGCGGCGCTGCGCCTGCTGCTGCGGGCCT
GGCCGGGCGAGGCACTGCCCCGCGCGCGGCTGATGGCCCTGGCGGTCGAACTGGGGGCCGATGTCCCGGTCTGCATCGAC
CAGCGCGCGGCCCGGATGGGCGGCGTGGGCGAAAGACTGGCCCCCGCGCCGGCGCTGCCGAACTGCGGCATGATGCTGGT
GAATTGCGGCGAGGCCGTGCCGACCCCCGCCGTGTTCCGCGCGCGGGCGCCCGTCTTCACCCCGGCGGCCAGCCTGCCGT
CCGCGTGGCCGGATGTCGGGGCGATGGTGCGCGACCTGGCGGCGCTGACCAACGACCTGCAGGATGCGGCCTGCGAGCTC
TGCCCGACCATCCGCACGGTCCTGCAGGTGCTGGACGCCGCGCCGGGCTGCCGGCTGGCAAGGATGAGCGGTTCGGGCGC
CACCTGCTTCGCCCTGTTCGACAGCCCGCAGGGGGCACGCGACGCGATGACGGCGGTCGAACGCCCCGGATGGTGGGTAT
GGGCCGGCGGCTTGCATGGAATGCCGGCGGAAACCTGA

Upstream 100 bases:

>100_bases
CAAAAGATCACTGCCGCCCTTGCGGACGCCGCCCGATCGGGCCACGAAGACCCGCCGCCCGACCAGGACGGGGCGGCCCG
GAAGAAGGACCCATCCGACC

Downstream 100 bases:

>100_bases
TAGACTGTCCCCCTCCGTTGGGGGGAGGATGGTATGAGCGCGCGCATGCAGGTCAGTCTGTCCGACGACGGCCGGACCGC
GAACTTGGCCTTTCTGGAAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTDPTGTGTLHESAHAKINLYLHVTGRRPDGYHLLDSLAVFAGAADRLTLRPGAAGQGEAVALDIAGAFGAGLVADTDSN
LVLQAARRLRAEMGVTDRLAPMRIVLEKSLPVASGIGGGSADAAAALRLLLRAWPGEALPRARLMALAVELGADVPVCID
QRAARMGGVGERLAPAPALPNCGMMLVNCGEAVPTPAVFRARAPVFTPAASLPSAWPDVGAMVRDLAALTNDLQDAACEL
CPTIRTVLQVLDAAPGCRLARMSGSGATCFALFDSPQGARDAMTAVERPGWWVWAGGLHGMPAET

Sequences:

>Translated_305_residues
MTDPTGTGTLHESAHAKINLYLHVTGRRPDGYHLLDSLAVFAGAADRLTLRPGAAGQGEAVALDIAGAFGAGLVADTDSN
LVLQAARRLRAEMGVTDRLAPMRIVLEKSLPVASGIGGGSADAAAALRLLLRAWPGEALPRARLMALAVELGADVPVCID
QRAARMGGVGERLAPAPALPNCGMMLVNCGEAVPTPAVFRARAPVFTPAASLPSAWPDVGAMVRDLAALTNDLQDAACEL
CPTIRTVLQVLDAAPGCRLARMSGSGATCFALFDSPQGARDAMTAVERPGWWVWAGGLHGMPAET
>Mature_304_residues
TDPTGTGTLHESAHAKINLYLHVTGRRPDGYHLLDSLAVFAGAADRLTLRPGAAGQGEAVALDIAGAFGAGLVADTDSNL
VLQAARRLRAEMGVTDRLAPMRIVLEKSLPVASGIGGGSADAAAALRLLLRAWPGEALPRARLMALAVELGADVPVCIDQ
RAARMGGVGERLAPAPALPNCGMMLVNCGEAVPTPAVFRARAPVFTPAASLPSAWPDVGAMVRDLAALTNDLQDAACELC
PTIRTVLQVLDAAPGCRLARMSGSGATCFALFDSPQGARDAMTAVERPGWWVWAGGLHGMPAET

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=30.9608540925267, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_GLUDA (A9HAC0)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601010.1
- RefSeq:   YP_002275680.1
- ProteinModelPortal:   A9HAC0
- SMR:   A9HAC0
- GeneID:   5789484
- GeneID:   6974688
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1283
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK14608
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31482; Mature: 31350

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: ACT_SITE 17-17 ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPTGTGTLHESAHAKINLYLHVTGRRPDGYHLLDSLAVFAGAADRLTLRPGAAGQGEA
CCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCE
VALDIAGAFGAGLVADTDSNLVLQAARRLRAEMGVTDRLAPMRIVLEKSLPVASGIGGGS
EEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCC
ADAAAALRLLLRAWPGEALPRARLMALAVELGADVPVCIDQRAARMGGVGERLAPAPALP
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
NCGMMLVNCGEAVPTPAVFRARAPVFTPAASLPSAWPDVGAMVRDLAALTNDLQDAACEL
CCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CPTIRTVLQVLDAAPGCRLARMSGSGATCFALFDSPQGARDAMTAVERPGWWVWAGGLHG
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCC
MPAET
CCCCC
>Mature Secondary Structure 
TDPTGTGTLHESAHAKINLYLHVTGRRPDGYHLLDSLAVFAGAADRLTLRPGAAGQGEA
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCE
VALDIAGAFGAGLVADTDSNLVLQAARRLRAEMGVTDRLAPMRIVLEKSLPVASGIGGGS
EEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCC
ADAAAALRLLLRAWPGEALPRARLMALAVELGADVPVCIDQRAARMGGVGERLAPAPALP
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
NCGMMLVNCGEAVPTPAVFRARAPVFTPAASLPSAWPDVGAMVRDLAALTNDLQDAACEL
CCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
CPTIRTVLQVLDAAPGCRLARMSGSGATCFALFDSPQGARDAMTAVERPGWWVWAGGLHG
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCC
MPAET
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA