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The map label for this gene is glnK [H]

Identifier: 162146531

GI number: 162146531

Start: 719774

End: 720112

Strand: Reverse

Name: glnK [H]

Synonym: GDI_0707

Alternate gene names: 162146531

Gene position: 720112-719774 (Counterclockwise)

Preceding gene: 162146532

Following gene: 162146530

Centisome position: 18.26

GC content: 53.69

Gene sequence:

>339_bases
ATGAAATTCATTATAGCCATTATCAAGCCATTCAAACTCGACGATGTCCGCGAAGCCCTGGGCACCATCGGCATCCAGGG
ACTGACCGTTACCGAAGTCAAAGGATATGGCCGCCAGAAGGGACAGACCGAGATCTATCGCGGCGCGGAATATCAGATCC
AGTTCGTGGCAAAAGTGAAAATCGAAGTCGCGGTGGCCGATTCGATGCTGGATCAGGCGCTCGATACCATTCGTGCCGCC
GCCCATACCGGCACGATCGGGGACGGCAAGATGTTTGTTCTTGAACTGCAACAGGCCATTCGTATCCGTACGAATGAAAC
CGGGGAGATTGCCTTGTGA

Upstream 100 bases:

>100_bases
GGAAATCCGGTAGCGGCTTCAGAACGATCTTGCGTCGAAACGTTTTGGCGAATGCAAGCATCGCAGCTTTCCTGGAGACA
TATTCGCCTGCGAGGAAGAC

Downstream 100 bases:

>100_bases
ACGGGTCAAATTCCTGCTTCAGACGATTGACGCTGGCGGCCCCGGCCGCCGCGATGGCCCTGCTGGCCGCCCCGTCGATC
GCATCCGCCGCCGATCCGGC

Product: regulatory protein GlnK2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKFIIAIIKPFKLDDVREALGTIGIQGLTVTEVKGYGRQKGQTEIYRGAEYQIQFVAKVKIEVAVADSMLDQALDTIRAA
AHTGTIGDGKMFVLELQQAIRIRTNETGEIAL

Sequences:

>Translated_112_residues
MKFIIAIIKPFKLDDVREALGTIGIQGLTVTEVKGYGRQKGQTEIYRGAEYQIQFVAKVKIEVAVADSMLDQALDTIRAA
AHTGTIGDGKMFVLELQQAIRIRTNETGEIAL
>Mature_112_residues
MKFIIAIIKPFKLDDVREALGTIGIQGLTVTEVKGYGRQKGQTEIYRGAEYQIQFVAKVKIEVAVADSMLDQALDTIRAA
AHTGTIGDGKMFVLELQQAIRIRTNETGEIAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=66.9642857142857, Blast_Score=157, Evalue=1e-40,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=62.5, Blast_Score=144, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12310; Mature: 12310

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIAIIKPFKLDDVREALGTIGIQGLTVTEVKGYGRQKGQTEIYRGAEYQIQFVAKVK
CEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCEEEEEEEEEE
IEVAVADSMLDQALDTIRAAAHTGTIGDGKMFVLELQQAIRIRTNETGEIAL
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFIIAIIKPFKLDDVREALGTIGIQGLTVTEVKGYGRQKGQTEIYRGAEYQIQFVAKVK
CEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCEEEEEEEEEE
IEVAVADSMLDQALDTIRAAAHTGTIGDGKMFVLELQQAIRIRTNETGEIAL
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]