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The map label for this gene is amtB [H]

Identifier: 162146530

GI number: 162146530

Start: 718398

End: 719777

Strand: Reverse

Name: amtB [H]

Synonym: GDI_0706

Alternate gene names: 162146530

Gene position: 719777-718398 (Counterclockwise)

Preceding gene: 162146531

Following gene: 162146529

Centisome position: 18.25

GC content: 67.25

Gene sequence:

>1380_bases
GTGAACGGGTCAAATTCCTGCTTCAGACGATTGACGCTGGCGGCCCCGGCCGCCGCGATGGCCCTGCTGGCCGCCCCGTC
GATCGCATCCGCCGCCGATCCGGCCCCTCCTCCCCCGATCAACACCGGCGATACCGCCTGGATGCTGACCAGCACCGCGC
TGGTGCTGATGATGACCGTGCCGGGCCTGGCCCTGTTCTATGGCGGCATGGTCCGCAAGAAGAACGTGCTGGCCACGCTG
ATGCAGTCCTTCGCCATCTGCTGCATCATCACCGTCCTGTGGATGGTCGCGGGCTACAGCCTGACGTTCGGCACCGGGTC
GCCCTATATCGGCGATCTGTCCCGCTTCATGCTGAACGGCATCGGCGCGCAGATTTCCAAGGGGTCCGATGTCGGCTTCA
CGCTGGGGCTGGGGTCGGCCAACGCCACGGTGATGACAATCCCCGAGAGCGTCTTCATGATGTTCCAGATGACGTTCGCG
ATCATCACCCCGGCGCTGATCGCCGGCTCGTTTGCCGAGCGCATGAAGTTCAGCGCGCTGTGCGTCTTCACCATCCTGTG
GTCGCTGCTGGTCTATGCGCCGATCGCCCACTGGGTCTGGAGCCCGCTGGGCTGGGTCGCGGGGTTCGGCGCCGTCGATT
TCGCCGGCGGCACCGTGGTGCACATCAATGCCGGCATCGCAGGCCTGGTCACGGCGCTGGTGCTGGGCAAGCGGCAGGGC
TACGGCCAGGACGATATGTCGCCCTTCAACCTGACCTATGCCGTCATCGGCGCGTCGCTACTATGGGTCGGCTGGTTCGG
CTTCAATGCCGGGTCGGCCGTGGGCTCCAACGGCCGCGCCGGCATGGCGATGGCGACGACGCAGATCGCGACCGCCGCCG
CCGGCCTGTCCTGGATGCTGGCCGAATGGGCCCGCACCGGCAAGCCGACGGTGCTGGGCATCATTTCCGGCGCGGTGGCG
GGCCTGGTCGCGATCACGCCCGCCGCCGGCTTCGTGCTGCCGGGCGGGGCGCTGGTCATCGGCCTGATCACGGGCGCCGT
GTGCTATTTCGCGGCGACGTCGCTGAAGCACATGCTGGGCTATGACGACAGCCTGGACGCCTTCGGCGTGCATGGCATCG
GCGGCATCCTGGGCGCGCTGCTGACCGGTGTGCTGGCCTATGGCCCGCTTTCGGCCACCGACGCCAACCCCGCCGGGGTC
GTGGGGTCGTTCGCGCAGTTCGTCACCCAGGCCAAGGCCGTGGGCGTCACCATCGTCTGGTGCGGCGTCGTGACCTTCAT
CCTGCTGAAGATCGTCGACCTCGCCATCGGCCTGCGCGTCACCACCGAACAGGAACAGCAGGGTCTCGACATGTCCCTCC
ATGGCGAAAAGATAAGCTGA

Upstream 100 bases:

>100_bases
CCGCCGCCCATACCGGCACGATCGGGGACGGCAAGATGTTTGTTCTTGAACTGCAACAGGCCATTCGTATCCGTACGAAT
GAAACCGGGGAGATTGCCTT

Downstream 100 bases:

>100_bases
TCGTTCCGGAAACGGGCGGCGCGTCATGCGCCACCCGTTAATATCAGAAAAATACAGTAATATTTAAGCAATACATAATA
ACGCACGAGATTTTCTCGGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNGSNSCFRRLTLAAPAAAMALLAAPSIASAADPAPPPPINTGDTAWMLTSTALVLMMTVPGLALFYGGMVRKKNVLATL
MQSFAICCIITVLWMVAGYSLTFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFA
IITPALIAGSFAERMKFSALCVFTILWSLLVYAPIAHWVWSPLGWVAGFGAVDFAGGTVVHINAGIAGLVTALVLGKRQG
YGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGSNGRAGMAMATTQIATAAAGLSWMLAEWARTGKPTVLGIISGAVA
GLVAITPAAGFVLPGGALVIGLITGAVCYFAATSLKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGV
VGSFAQFVTQAKAVGVTIVWCGVVTFILLKIVDLAIGLRVTTEQEQQGLDMSLHGEKIS

Sequences:

>Translated_459_residues
MNGSNSCFRRLTLAAPAAAMALLAAPSIASAADPAPPPPINTGDTAWMLTSTALVLMMTVPGLALFYGGMVRKKNVLATL
MQSFAICCIITVLWMVAGYSLTFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFA
IITPALIAGSFAERMKFSALCVFTILWSLLVYAPIAHWVWSPLGWVAGFGAVDFAGGTVVHINAGIAGLVTALVLGKRQG
YGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGSNGRAGMAMATTQIATAAAGLSWMLAEWARTGKPTVLGIISGAVA
GLVAITPAAGFVLPGGALVIGLITGAVCYFAATSLKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGV
VGSFAQFVTQAKAVGVTIVWCGVVTFILLKIVDLAIGLRVTTEQEQQGLDMSLHGEKIS
>Mature_459_residues
MNGSNSCFRRLTLAAPAAAMALLAAPSIASAADPAPPPPINTGDTAWMLTSTALVLMMTVPGLALFYGGMVRKKNVLATL
MQSFAICCIITVLWMVAGYSLTFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFA
IITPALIAGSFAERMKFSALCVFTILWSLLVYAPIAHWVWSPLGWVAGFGAVDFAGGTVVHINAGIAGLVTALVLGKRQG
YGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGSNGRAGMAMATTQIATAAAGLSWMLAEWARTGKPTVLGIISGAVA
GLVAITPAAGFVLPGGALVIGLITGAVCYFAATSLKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGV
VGSFAQFVTQAKAVGVTIVWCGVVTFILLKIVDLAIGLRVTTEQEQQGLDMSLHGEKIS

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=426, Percent_Identity=46.4788732394366, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=27.3942093541203, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=449, Percent_Identity=27.8396436525612, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=430, Percent_Identity=26.9767441860465, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=441, Percent_Identity=25.8503401360544, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=36.8298368298368, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=33.9578454332553, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=30.4038004750594, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI161078314, Length=425, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47199; Mature: 47199

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSNSCFRRLTLAAPAAAMALLAAPSIASAADPAPPPPINTGDTAWMLTSTALVLMMTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PGLALFYGGMVRKKNVLATLMQSFAICCIITVLWMVAGYSLTFGTGSPYIGDLSRFMLNG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHH
IGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFAIITPALIAGSFAERMKFSAL
CCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVFTILWSLLVYAPIAHWVWSPLGWVAGFGAVDFAGGTVVHINAGIAGLVTALVLGKRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCC
YGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGSNGRAGMAMATTQIATAAAGLSWML
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEWARTGKPTVLGIISGAVAGLVAITPAAGFVLPGGALVIGLITGAVCYFAATSLKHMLG
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
YDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGVVGSFAQFVTQAKAVGVTIVW
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
CGVVTFILLKIVDLAIGLRVTTEQEQQGLDMSLHGEKIS
HHHHHHHHHHHHHHHHCCEEECCHHHCCCCEECCCCCCC
>Mature Secondary Structure
MNGSNSCFRRLTLAAPAAAMALLAAPSIASAADPAPPPPINTGDTAWMLTSTALVLMMTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PGLALFYGGMVRKKNVLATLMQSFAICCIITVLWMVAGYSLTFGTGSPYIGDLSRFMLNG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHH
IGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFAIITPALIAGSFAERMKFSAL
CCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVFTILWSLLVYAPIAHWVWSPLGWVAGFGAVDFAGGTVVHINAGIAGLVTALVLGKRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCC
YGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGSNGRAGMAMATTQIATAAAGLSWML
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEWARTGKPTVLGIISGAVAGLVAITPAAGFVLPGGALVIGLITGAVCYFAATSLKHMLG
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
YDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGVVGSFAQFVTQAKAVGVTIVW
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
CGVVTFILLKIVDLAIGLRVTTEQEQQGLDMSLHGEKIS
HHHHHHHHHHHHHHHHCCEEECCHHHCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]