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The map label for this gene is stp [H]

Identifier: 162146514

GI number: 162146514

Start: 700568

End: 702157

Strand: Direct

Name: stp [H]

Synonym: GDI_0690

Alternate gene names: 162146514

Gene position: 700568-702157 (Clockwise)

Preceding gene: 162146511

Following gene: 162146516

Centisome position: 17.76

GC content: 71.95

Gene sequence:

>1590_bases
CTGCTCGTGACTGGACTGTTCGTGACTGGGCGGCCGACGGCGGGATTCCTATTGTCGCGGGGCATGACCGACCTGTCCCC
CCATCCCGCTCTGCGTTCCGATCTTCGTCCGGGCCTGGTGCTGGCCACCTGTTGCCTCAGCCTGCTGCTGGTGATGATGG
ACGTGACCATCGTCAATGTCGCGCTGCCGTCGATTCGCGCGGCGCTGGGCGGGCGGTTTTCGAACCTGCAATGGGTGGTG
GACGCCTACACGCTGGTCGTGGCCACCCTGCTGGTCCTGGCGGGGTCGACGGCCGACCGGATCGGCCGGCGCCGCGTCTT
CGTCACGGGAATCGCGATCTTCTGCCTGGGGTCGGCGCTGTGCGGCCTGGCGCCCAGCGCGCGGGCCCTGGTGATGGCGC
GCGCGTTCCAGGGGCTGGGCGGCGCGATGCTGAATCCCGTGGCGCTGTCGATCATCGCCAATGTCTTCGTCACCCCCGTG
GCCCGGGCGCGGGCGATCGGCATGTGGGGCGGCATGTCGGGGCTGGCACTGGCGCTGGGGCCGCTGGCGGGCGGCGTGCT
GACCACGCTGGCCGGCTGGCGGGCCGTGTTCTGGGTCAACCTGCCGATCGGACTGGTGGCGATGGTCCTCAGCCTGCGCT
TCATTCCCGAATCGCGGGCGGCGCGGGTACGGCCGATCGACGTGCCGGCGCAGGTGCTGATCGCCCTGGTCCTGGCGGCG
ACGACCGCGGCACTGATCGAAGCCCGCAACTACGGCTGGGGCGCCCCGGCCATCCAGGCGACGCTCGCCCTCGCGGCGAT
GGGGATGGTGGCGCTGGTCGTGGTGGAACGCCGGCGGGCGCAGCCGCTGCTGGATCTGCGCTTCTTCCGTGCCCTGCCGT
TCACCGGGGCGATCCTGATCGCGATCTGCGCCTTCGCGATCTTCAGTTCCTTCCTGTTCCTGAACACGCTGTACCTGCAG
GACGTGCGCGGCCTGTCCGCCCTGCGGGCCGGGCTGTACACGCTGCCTTTCGCCATGGGGGTGATGGCCTGCGCGCCGCT
GTCGGGCCGCATCGTGGGGGCGCACGGCGCGCGGTTGCCGCTGCTGCTGTCGGCGCTGGGGCTGGGGGGCGGGGCGATAT
TGCTGACCGGTCTGACGGCGACCACACCGCCCGCGGTGCTGCTGGCCGCCTACGGCCTGTGCGGCTGTGGATTCGGCCTG
TGCAACGCGCCGATCACCAATGCGGCGGTTTCGGGCATGCCGCGCGACCAGGCGGGGCTGGCGGCGGCCCTGGCCTCGAC
CAGCCGGCAGATCGGCGCCCTGCTGGGGATCGCGGCATCGGGGGCGATGACGGCATCGGTCATGTCCGCCCCGGTCCAGC
CGGCGCCGCTGTCATCAGCGGGCGCCGTCGTCGCGTACATGGCACTGCTGCCGGCGGCCAGCCATCGGGTCTGGTGGACC
ATCGTCGTCCTGGCCTGCGGCATCGCCGGCCTGGGCGTGGTGTCGACGGGCGCGCGCGCCCGGCGCAGCGCGCAGACGGT
CCGCGCGCTGTTCGATGACGGGCCGCAGGCGTCCGGTTACAGGACGGACAGCACCCCGCCATCGACATAA

Upstream 100 bases:

>100_bases
GGTTCGATTCCCTTCGCCCGCTCCATCTCCCCGCTGCTTTTTTCCAGAAAATCGATTCGATCCGGCTCCGGACCGGCGTG
ATCGCATCCGCGTGACCGGG

Downstream 100 bases:

>100_bases
AGCGTCTGGCCGTTGACGAAGGACGACGCGTCGGACGCCAGGAAGACCGCCGCGCCCTGCAGTTCCTCCACCCGGCCCCA
GCGCCCGGCCGGCGTGCGCT

Product: MFS transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLVTGLFVTGRPTAGFLLSRGMTDLSPHPALRSDLRPGLVLATCCLSLLLVMMDVTIVNVALPSIRAALGGRFSNLQWVV
DAYTLVVATLLVLAGSTADRIGRRRVFVTGIAIFCLGSALCGLAPSARALVMARAFQGLGGAMLNPVALSIIANVFVTPV
ARARAIGMWGGMSGLALALGPLAGGVLTTLAGWRAVFWVNLPIGLVAMVLSLRFIPESRAARVRPIDVPAQVLIALVLAA
TTAALIEARNYGWGAPAIQATLALAAMGMVALVVVERRRAQPLLDLRFFRALPFTGAILIAICAFAIFSSFLFLNTLYLQ
DVRGLSALRAGLYTLPFAMGVMACAPLSGRIVGAHGARLPLLLSALGLGGGAILLTGLTATTPPAVLLAAYGLCGCGFGL
CNAPITNAAVSGMPRDQAGLAAALASTSRQIGALLGIAASGAMTASVMSAPVQPAPLSSAGAVVAYMALLPAASHRVWWT
IVVLACGIAGLGVVSTGARARRSAQTVRALFDDGPQASGYRTDSTPPST

Sequences:

>Translated_529_residues
MLVTGLFVTGRPTAGFLLSRGMTDLSPHPALRSDLRPGLVLATCCLSLLLVMMDVTIVNVALPSIRAALGGRFSNLQWVV
DAYTLVVATLLVLAGSTADRIGRRRVFVTGIAIFCLGSALCGLAPSARALVMARAFQGLGGAMLNPVALSIIANVFVTPV
ARARAIGMWGGMSGLALALGPLAGGVLTTLAGWRAVFWVNLPIGLVAMVLSLRFIPESRAARVRPIDVPAQVLIALVLAA
TTAALIEARNYGWGAPAIQATLALAAMGMVALVVVERRRAQPLLDLRFFRALPFTGAILIAICAFAIFSSFLFLNTLYLQ
DVRGLSALRAGLYTLPFAMGVMACAPLSGRIVGAHGARLPLLLSALGLGGGAILLTGLTATTPPAVLLAAYGLCGCGFGL
CNAPITNAAVSGMPRDQAGLAAALASTSRQIGALLGIAASGAMTASVMSAPVQPAPLSSAGAVVAYMALLPAASHRVWWT
IVVLACGIAGLGVVSTGARARRSAQTVRALFDDGPQASGYRTDSTPPST
>Mature_529_residues
MLVTGLFVTGRPTAGFLLSRGMTDLSPHPALRSDLRPGLVLATCCLSLLLVMMDVTIVNVALPSIRAALGGRFSNLQWVV
DAYTLVVATLLVLAGSTADRIGRRRVFVTGIAIFCLGSALCGLAPSARALVMARAFQGLGGAMLNPVALSIIANVFVTPV
ARARAIGMWGGMSGLALALGPLAGGVLTTLAGWRAVFWVNLPIGLVAMVLSLRFIPESRAARVRPIDVPAQVLIALVLAA
TTAALIEARNYGWGAPAIQATLALAAMGMVALVVVERRRAQPLLDLRFFRALPFTGAILIAICAFAIFSSFLFLNTLYLQ
DVRGLSALRAGLYTLPFAMGVMACAPLSGRIVGAHGARLPLLLSALGLGGGAILLTGLTATTPPAVLLAAYGLCGCGFGL
CNAPITNAAVSGMPRDQAGLAAALASTSRQIGALLGIAASGAMTASVMSAPVQPAPLSSAGAVVAYMALLPAASHRVWWT
IVVLACGIAGLGVVSTGARARRSAQTVRALFDDGPQASGYRTDSTPPST

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=405, Percent_Identity=29.8765432098765, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=398, Percent_Identity=26.3819095477387, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790195, Length=395, Percent_Identity=28.6075949367089, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789042, Length=416, Percent_Identity=26.2019230769231, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=28.992628992629, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=396, Percent_Identity=25, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=194, Percent_Identity=31.4432989690722, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323520, Length=352, Percent_Identity=24.4318181818182, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=151, Percent_Identity=31.7880794701987, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=314, Percent_Identity=25.1592356687898, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=157, Percent_Identity=27.3885350318471, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54184; Mature: 54184

Theoretical pI: Translated: 11.61; Mature: 11.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTGLFVTGRPTAGFLLSRGMTDLSPHPALRSDLRPGLVLATCCLSLLLVMMDVTIVNV
CEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPSIRAALGGRFSNLQWVVDAYTLVVATLLVLAGSTADRIGRRRVFVTGIAIFCLGSAL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHH
CGLAPSARALVMARAFQGLGGAMLNPVALSIIANVFVTPVARARAIGMWGGMSGLALALG
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
PLAGGVLTTLAGWRAVFWVNLPIGLVAMVLSLRFIPESRAARVRPIDVPAQVLIALVLAA
HHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
TTAALIEARNYGWGAPAIQATLALAAMGMVALVVVERRRAQPLLDLRFFRALPFTGAILI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
AICAFAIFSSFLFLNTLYLQDVRGLSALRAGLYTLPFAMGVMACAPLSGRIVGAHGARLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHH
LLLSALGLGGGAILLTGLTATTPPAVLLAAYGLCGCGFGLCNAPITNAAVSGMPRDQAGL
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHCCCCCCHHHH
AAALASTSRQIGALLGIAASGAMTASVMSAPVQPAPLSSAGAVVAYMALLPAASHRVWWT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
IVVLACGIAGLGVVSTGARARRSAQTVRALFDDGPQASGYRTDSTPPST
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLVTGLFVTGRPTAGFLLSRGMTDLSPHPALRSDLRPGLVLATCCLSLLLVMMDVTIVNV
CEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPSIRAALGGRFSNLQWVVDAYTLVVATLLVLAGSTADRIGRRRVFVTGIAIFCLGSAL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHH
CGLAPSARALVMARAFQGLGGAMLNPVALSIIANVFVTPVARARAIGMWGGMSGLALALG
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
PLAGGVLTTLAGWRAVFWVNLPIGLVAMVLSLRFIPESRAARVRPIDVPAQVLIALVLAA
HHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
TTAALIEARNYGWGAPAIQATLALAAMGMVALVVVERRRAQPLLDLRFFRALPFTGAILI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
AICAFAIFSSFLFLNTLYLQDVRGLSALRAGLYTLPFAMGVMACAPLSGRIVGAHGARLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHH
LLLSALGLGGGAILLTGLTATTPPAVLLAAYGLCGCGFGLCNAPITNAAVSGMPRDQAGL
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHHHHHCCCCCCHHHH
AAALASTSRQIGALLGIAASGAMTASVMSAPVQPAPLSSAGAVVAYMALLPAASHRVWWT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
IVVLACGIAGLGVVSTGARARRSAQTVRALFDDGPQASGYRTDSTPPST
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]