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The map label for this gene is lacI [H]

Identifier: 162146516

GI number: 162146516

Start: 703145

End: 704224

Strand: Direct

Name: lacI [H]

Synonym: GDI_0692

Alternate gene names: 162146516

Gene position: 703145-704224 (Clockwise)

Preceding gene: 162146514

Following gene: 162146517

Centisome position: 17.83

GC content: 70.46

Gene sequence:

>1080_bases
ATGCCGGAACACGCGATATCCGAGGCACGGCCGCGTGCCGCCCGGCCCACCATCATGGATGTCTCGGCGCGGGCGGGGGT
GTCGCGCATGACGGTCTCCCGCGCCTTGCGGACGCCGGACCTGGTCCGCGAGGACACGCGGCGGGCCATCGAACGCGCCA
TTGCCGACCTGGGCTATGTGCCCGACCGGGCGGCCGAGGCCCTGTCGACCCGCCGCAGCGGTTTCGTCGGGCTGGTGGTG
CCGACCCTGACCAACACCAATTTCGCCGATGCGGCGCGGGGGCTGACGGACATCATCCGCCGCGACGGGTACGAATTGCT
GATTGGGTATACCGATTATGACGAAAACGCAATGCTGCGCCACGTGCGCGACATGCTGGGCCGCCGGGCGGAGGCCCTGA
TCCTGCCGGCCGGTCCGCGCGGGCCGGCGTTACGGGGCATGCTGGCGTCGGAAACCATTCCCATCGTACAGATGGCGGGC
CTGGCCGGCCGGCCGGTCCATCGCATGGTCGGCTATTCGAACCGCGAGGCCGGGCGCATGGCCGCACACCACCTGATCGC
GCTGGGCCATACGCGCATCGGTGCCCTGGGGGCGGAGGGTGACGGCCTGTCCTGCGATCATCGCGGCGGCGAGCGGCTGG
CGGGCTTCGCCGAGGCCCTGCGTGAGGCCGGATTGTCCGACGCGCTTGTGCTGCGCATGGGCAATCCGCCGATTTCCTTT
ACCCATGGCGCGCAGGCCATGGGGCATCTGCTGAACCGGGAACCGGACGTGCAGGCGGTGTTCGCGGTGTCCGACCTGGT
GGGGGTCGGCGCGCTGATGGAATGCCACCGAAGGGGCGTGCGCGTTCCGCAGGATATATCGCTGATCGGGTTCGGCGGGT
TCGAGGTCGGCCGCCAGATGGTGCCCGCCCTGACCACGGTCGCGGTCGATTTCCGCGCGATGGGCGAACGGACGGGGCGC
ATGGTGCTGGACCTGCTGGCCGGCCGCCCCGGGGTGCCCCGCATCGTCGATCTGGGCGTGGCGCTGGTCGAACGCGAAAC
GACGGCCTTCCGTCCGTCCGCGCTGTCCATCACCGCCTGA

Upstream 100 bases:

>100_bases
GCGGGAAGGGTGTGAAACGGGCATGACAGGCAGGCGGACGACAGGAACAGGCCCGACGGCTGGGCCGGAGGTGCCGGGGC
AGGAAGGGCCAGGGCAGGGG

Downstream 100 bases:

>100_bases
AGGACCGCGGGCCGAACAGGCGCCCGCGCGCCCGCTGCAATGGCGGTGCCAACGGCGGAGCGGAAGGCGTTGCCAGGCGC
GCGGGACCGCGTCATTGTCC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPEHAISEARPRAARPTIMDVSARAGVSRMTVSRALRTPDLVREDTRRAIERAIADLGYVPDRAAEALSTRRSGFVGLVV
PTLTNTNFADAARGLTDIIRRDGYELLIGYTDYDENAMLRHVRDMLGRRAEALILPAGPRGPALRGMLASETIPIVQMAG
LAGRPVHRMVGYSNREAGRMAAHHLIALGHTRIGALGAEGDGLSCDHRGGERLAGFAEALREAGLSDALVLRMGNPPISF
THGAQAMGHLLNREPDVQAVFAVSDLVGVGALMECHRRGVRVPQDISLIGFGGFEVGRQMVPALTTVAVDFRAMGERTGR
MVLDLLAGRPGVPRIVDLGVALVERETTAFRPSALSITA

Sequences:

>Translated_359_residues
MPEHAISEARPRAARPTIMDVSARAGVSRMTVSRALRTPDLVREDTRRAIERAIADLGYVPDRAAEALSTRRSGFVGLVV
PTLTNTNFADAARGLTDIIRRDGYELLIGYTDYDENAMLRHVRDMLGRRAEALILPAGPRGPALRGMLASETIPIVQMAG
LAGRPVHRMVGYSNREAGRMAAHHLIALGHTRIGALGAEGDGLSCDHRGGERLAGFAEALREAGLSDALVLRMGNPPISF
THGAQAMGHLLNREPDVQAVFAVSDLVGVGALMECHRRGVRVPQDISLIGFGGFEVGRQMVPALTTVAVDFRAMGERTGR
MVLDLLAGRPGVPRIVDLGVALVERETTAFRPSALSITA
>Mature_358_residues
PEHAISEARPRAARPTIMDVSARAGVSRMTVSRALRTPDLVREDTRRAIERAIADLGYVPDRAAEALSTRRSGFVGLVVP
TLTNTNFADAARGLTDIIRRDGYELLIGYTDYDENAMLRHVRDMLGRRAEALILPAGPRGPALRGMLASETIPIVQMAGL
AGRPVHRMVGYSNREAGRMAAHHLIALGHTRIGALGAEGDGLSCDHRGGERLAGFAEALREAGLSDALVLRMGNPPISFT
HGAQAMGHLLNREPDVQAVFAVSDLVGVGALMECHRRGVRVPQDISLIGFGGFEVGRQMVPALTTVAVDFRAMGERTGRM
VLDLLAGRPGVPRIVDLGVALVERETTAFRPSALSITA

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=32.02416918429, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790715, Length=338, Percent_Identity=31.0650887573964, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=29.0123456790123, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=30.5882352941176, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788474, Length=356, Percent_Identity=31.4606741573034, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1790194, Length=322, Percent_Identity=28.8819875776398, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=30.9734513274336, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789068, Length=327, Percent_Identity=28.1345565749235, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=27.7456647398844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787580, Length=336, Percent_Identity=23.8095238095238, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787906, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=26.9794721407625, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38481; Mature: 38350

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHAISEARPRAARPTIMDVSARAGVSRMTVSRALRTPDLVREDTRRAIERAIADLGYV
CCCCCHHHCCCCCCCCCEEECHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PDRAAEALSTRRSGFVGLVVPTLTNTNFADAARGLTDIIRRDGYELLIGYTDYDENAMLR
CHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
HVRDMLGRRAEALILPAGPRGPALRGMLASETIPIVQMAGLAGRPVHRMVGYSNREAGRM
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCCHHHH
AAHHLIALGHTRIGALGAEGDGLSCDHRGGERLAGFAEALREAGLSDALVLRMGNPPISF
HHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
THGAQAMGHLLNREPDVQAVFAVSDLVGVGALMECHRRGVRVPQDISLIGFGGFEVGRQM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHH
VPALTTVAVDFRAMGERTGRMVLDLLAGRPGVPRIVDLGVALVERETTAFRPSALSITA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECC
>Mature Secondary Structure 
PEHAISEARPRAARPTIMDVSARAGVSRMTVSRALRTPDLVREDTRRAIERAIADLGYV
CCCCHHHCCCCCCCCCEEECHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PDRAAEALSTRRSGFVGLVVPTLTNTNFADAARGLTDIIRRDGYELLIGYTDYDENAMLR
CHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
HVRDMLGRRAEALILPAGPRGPALRGMLASETIPIVQMAGLAGRPVHRMVGYSNREAGRM
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCCHHHH
AAHHLIALGHTRIGALGAEGDGLSCDHRGGERLAGFAEALREAGLSDALVLRMGNPPISF
HHHHHHHHCCHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
THGAQAMGHLLNREPDVQAVFAVSDLVGVGALMECHRRGVRVPQDISLIGFGGFEVGRQM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHH
VPALTTVAVDFRAMGERTGRMVLDLLAGRPGVPRIVDLGVALVERETTAFRPSALSITA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]