Click here to switch to the map view.

The map label for this gene is sbp [H]

Identifier: 162146383

GI number: 162146383

Start: 564287

End: 565318

Strand: Direct

Name: sbp [H]

Synonym: GDI_0558

Alternate gene names: 162146383

Gene position: 564287-565318 (Clockwise)

Preceding gene: 162146382

Following gene: 162146384

Centisome position: 14.31

GC content: 70.16

Gene sequence:

>1032_bases
ATGACGAACGTTTTCCCCTCTTCCTCGCGCCGGGCGTTTCTTGCCGGCGCGGCCGCCGGTGCGGCGTCCCTCGCGGTGGG
GCCGTGCCCGGCCCGGGCCGCCACTCCCCGGCTGCTCAACGTGTCCTACGATCCGACGCGCGAACTCTATGCCGATGTCA
GCCGCGCCTTCGCCGCGTCCTGGGCGGGCGCGCATGGCGGCGAGCGCGTCGTGGTGACCAGTTCGCATGGCGGTTCGGGC
GCCCAGGCGCGTTCGGTGCTGGAGGGCGCGCCCGCCGACGTCGTGACGCTGGGCCTGGCCTACGACATCGACATCCTGGC
GCAGAACGGGCTGCTGGCCGCCGACTGGCAGCAGCGCCTGCCGCACAATTCCACACCTTATACCAGCACCATCGTCTTCC
TGGTGCGCAAGGGGAACCCCAAGGGCATCCATGACTGGCCCGACCTGATCCGCGACGGCGTGCAGGTCATCACCCCCAAT
CCCAAGACCTCCGGCGGCGCGCGCTGGAACTACCTGGCCGCCTGGGGCTGGGCCCTGCGGCAGCCCGGCGGGTCCGAGGC
GTCGGCACGGGCGTACCTCAAGGCCCTGTTCGGCCATGTGCCGGTGCTGGATACCGGCGCGCGCGGGGCGACCAACAGCT
TCGTGCAGCGCGGGCTGGGCGACGTGCTGATCGCGTGGGAGGACGAGGCCCTGCTGGCCTCGCGCGACCTGGGGCCGGAC
AAGTTCGACATCGTCGTTCCCCCCCTTACCATCCTGGCCGAACCGCCCGTCGCGGTGGTGGACCGCAACGTGCAGGCGCA
CGGCACGGCAGCGGTGGCCGAGGCCTATCTGCGGTTCCTGTTCACGCCGGAAGGCCAGCGGATCGGGGTGAAGCATTATT
TCCGGCCCGTCGATCCCGCGATCATCGCCGAAAGCGCGTCGGTCTTCCCGAAGACCACGGTTTTCGACGTCGCCAGCCTG
GGCGGATGGACCCGCGTGCAGAAGACGCATTTCGCCGACGGCGGCGTGTTCGACCAGATCTACAGCCGGTAA

Upstream 100 bases:

>100_bases
GCGTCAACCTCCTGGGCCGCAGCGGCAATGCCGTGATGGGCCGGGTCCAGGCCTCGTTCTGATCCTTCCGGTTCCCCGGA
TACGGCTTTCAAGGACAGTC

Downstream 100 bases:

>100_bases
CGGGTTCCGTCATGGCGTTTCGCCTGCGGCGGCGCGACGCGCTGCCGGGTTTTTCGGTCTCGATCGGCTTCACGCTGGTC
TGGATCGGGCTGATCGTGGC

Product: sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTNVFPSSSRRAFLAGAAAGAASLAVGPCPARAATPRLLNVSYDPTRELYADVSRAFAASWAGAHGGERVVVTSSHGGSG
AQARSVLEGAPADVVTLGLAYDIDILAQNGLLAADWQQRLPHNSTPYTSTIVFLVRKGNPKGIHDWPDLIRDGVQVITPN
PKTSGGARWNYLAAWGWALRQPGGSEASARAYLKALFGHVPVLDTGARGATNSFVQRGLGDVLIAWEDEALLASRDLGPD
KFDIVVPPLTILAEPPVAVVDRNVQAHGTAAVAEAYLRFLFTPEGQRIGVKHYFRPVDPAIIAESASVFPKTTVFDVASL
GGWTRVQKTHFADGGVFDQIYSR

Sequences:

>Translated_343_residues
MTNVFPSSSRRAFLAGAAAGAASLAVGPCPARAATPRLLNVSYDPTRELYADVSRAFAASWAGAHGGERVVVTSSHGGSG
AQARSVLEGAPADVVTLGLAYDIDILAQNGLLAADWQQRLPHNSTPYTSTIVFLVRKGNPKGIHDWPDLIRDGVQVITPN
PKTSGGARWNYLAAWGWALRQPGGSEASARAYLKALFGHVPVLDTGARGATNSFVQRGLGDVLIAWEDEALLASRDLGPD
KFDIVVPPLTILAEPPVAVVDRNVQAHGTAAVAEAYLRFLFTPEGQRIGVKHYFRPVDPAIIAESASVFPKTTVFDVASL
GGWTRVQKTHFADGGVFDQIYSR
>Mature_342_residues
TNVFPSSSRRAFLAGAAAGAASLAVGPCPARAATPRLLNVSYDPTRELYADVSRAFAASWAGAHGGERVVVTSSHGGSGA
QARSVLEGAPADVVTLGLAYDIDILAQNGLLAADWQQRLPHNSTPYTSTIVFLVRKGNPKGIHDWPDLIRDGVQVITPNP
KTSGGARWNYLAAWGWALRQPGGSEASARAYLKALFGHVPVLDTGARGATNSFVQRGLGDVLIAWEDEALLASRDLGPDK
FDIVVPPLTILAEPPVAVVDRNVQAHGTAAVAEAYLRFLFTPEGQRIGVKHYFRPVDPAIIAESASVFPKTTVFDVASLG
GWTRVQKTHFADGGVFDQIYSR

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=308, Percent_Identity=60.0649350649351, Blast_Score=396, Evalue=1e-112,
Organism=Escherichia coli, GI1788765, Length=306, Percent_Identity=47.3856209150327, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36447; Mature: 36316

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVFPSSSRRAFLAGAAAGAASLAVGPCPARAATPRLLNVSYDPTRELYADVSRAFAAS
CCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
WAGAHGGERVVVTSSHGGSGAQARSVLEGAPADVVTLGLAYDIDILAQNGLLAADWQQRL
HCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEEEEEEEECCCEEEECHHHHC
PHNSTPYTSTIVFLVRKGNPKGIHDWPDLIRDGVQVITPNPKTSGGARWNYLAAWGWALR
CCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCEEEHEEHHHHHC
QPGGSEASARAYLKALFGHVPVLDTGARGATNSFVQRGLGDVLIAWEDEALLASRDLGPD
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCEEEEECCCHHHHHCCCCCC
KFDIVVPPLTILAEPPVAVVDRNVQAHGTAAVAEAYLRFLFTPEGQRIGVKHYFRPVDPA
CEEEEECCHHEECCCCHHEECCCCCCCCHHHHHHHHHHHHCCCCCCEECHHHHCCCCCHH
IIAESASVFPKTTVFDVASLGGWTRVQKTHFADGGVFDQIYSR
HEECCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure 
TNVFPSSSRRAFLAGAAAGAASLAVGPCPARAATPRLLNVSYDPTRELYADVSRAFAAS
CCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
WAGAHGGERVVVTSSHGGSGAQARSVLEGAPADVVTLGLAYDIDILAQNGLLAADWQQRL
HCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEEEEEEEECCCEEEECHHHHC
PHNSTPYTSTIVFLVRKGNPKGIHDWPDLIRDGVQVITPNPKTSGGARWNYLAAWGWALR
CCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCEEEHEEHHHHHC
QPGGSEASARAYLKALFGHVPVLDTGARGATNSFVQRGLGDVLIAWEDEALLASRDLGPD
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCEEEEECCCHHHHHCCCCCC
KFDIVVPPLTILAEPPVAVVDRNVQAHGTAAVAEAYLRFLFTPEGQRIGVKHYFRPVDPA
CEEEEECCHHEECCCCHHEECCCCCCCCHHHHHHHHHHHHCCCCCCEECHHHHCCCCCHH
IIAESASVFPKTTVFDVASLGGWTRVQKTHFADGGVFDQIYSR
HEECCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]