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The map label for this gene is ygfA [C]

Identifier: 162146341

GI number: 162146341

Start: 510313

End: 510957

Strand: Direct

Name: ygfA [C]

Synonym: GDI_0516

Alternate gene names: 162146341

Gene position: 510313-510957 (Clockwise)

Preceding gene: 162146340

Following gene: 162146342

Centisome position: 12.94

GC content: 72.87

Gene sequence:

>645_bases
GTGAGCAGGTCCGGGAGGGCTGAAAGACCAACGGCCATGGTGGCTCCGCACATTCTTCCCCAACACGATTCCGCATCCGA
CGCGCCCGCGCTGGCCGAGGCCAAGGCGGCGTTGCGCCGGCGGCTGCGCGCCCTTCGCGCCGCCCCGCTGCCGGCGGACA
CGCCTGCCGCGCTGTGCCGGCGCATGGCCGATTGCGTCCGTGCCGGCTTGCGCCCGGGCGCCACGGTTGCCGCCGTATGG
CCCCTTCCGGGTGAAATCGACCTGCGCGGCCTGTGCGCCGAACTGCATCACGGCGGCTATGGCGTGCTGCTGCCCGAAAC
GCCCCCGCGCGGCCATCCCCTGCGCTTTCGCCTGTGGCGCCCGGGCGTGCCGATGCAGGCGGGCCGGTTCGGCACGCTTC
ATCCCGACGGCCCGGTCGCGCGCCCCGATTTCGTGTTCGTGCCGCTGCTGGGCTTTGACAGGCGGGGCTTCCGGCTGGGA
TACGGGGGCGGCTATTACGACCGCACGCTGGCCGGACTGGATTCGGTGCCGGCGGCGGGCTACGGGCTGTCGGCACAGGA
AGTGCCGGCGGTGCCGGTGGGGCGTCATGACGTCCCCCTGCCGGTCATCGTGACCGAACGCGAAACCATCCGCTGCGGCG
CCTGA

Upstream 100 bases:

>100_bases
TACCCGGCTTGTCAGGCAACTCAACCCCTGGGCCGATAAGCACTTCCTTAGGGAGCTGGCTCTGGCGTGATCCTGGTCAT
GTTATCTGGCGCCCACCTGC

Downstream 100 bases:

>100_bases
CGTCCGGGGCGGACGACGGGAAAAGGAAGGCCGGGTGCGGATCTTGTTTCTGGGTGATATCGTCGGGCGCACGGGGCGCG
ACGCCGTCATCGCCGCGCTG

Product: 5-formyltetrahydrofolate cyclo-ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSRSGRAERPTAMVAPHILPQHDSASDAPALAEAKAALRRRLRALRAAPLPADTPAALCRRMADCVRAGLRPGATVAAVW
PLPGEIDLRGLCAELHHGGYGVLLPETPPRGHPLRFRLWRPGVPMQAGRFGTLHPDGPVARPDFVFVPLLGFDRRGFRLG
YGGGYYDRTLAGLDSVPAAGYGLSAQEVPAVPVGRHDVPLPVIVTERETIRCGA

Sequences:

>Translated_214_residues
MSRSGRAERPTAMVAPHILPQHDSASDAPALAEAKAALRRRLRALRAAPLPADTPAALCRRMADCVRAGLRPGATVAAVW
PLPGEIDLRGLCAELHHGGYGVLLPETPPRGHPLRFRLWRPGVPMQAGRFGTLHPDGPVARPDFVFVPLLGFDRRGFRLG
YGGGYYDRTLAGLDSVPAAGYGLSAQEVPAVPVGRHDVPLPVIVTERETIRCGA
>Mature_213_residues
SRSGRAERPTAMVAPHILPQHDSASDAPALAEAKAALRRRLRALRAAPLPADTPAALCRRMADCVRAGLRPGATVAAVWP
LPGEIDLRGLCAELHHGGYGVLLPETPPRGHPLRFRLWRPGVPMQAGRFGTLHPDGPVARPDFVFVPLLGFDRRGFRLGY
GGGYYDRTLAGLDSVPAAGYGLSAQEVPAVPVGRHDVPLPVIVTERETIRCGA

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=140, Percent_Identity=35, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 22875; Mature: 22744

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSGRAERPTAMVAPHILPQHDSASDAPALAEAKAALRRRLRALRAAPLPADTPAALCR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RMADCVRAGLRPGATVAAVWPLPGEIDLRGLCAELHHGGYGVLLPETPPRGHPLRFRLWR
HHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEC
PGVPMQAGRFGTLHPDGPVARPDFVFVPLLGFDRRGFRLGYGGGYYDRTLAGLDSVPAAG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCC
YGLSAQEVPAVPVGRHDVPLPVIVTERETIRCGA
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCC
>Mature Secondary Structure 
SRSGRAERPTAMVAPHILPQHDSASDAPALAEAKAALRRRLRALRAAPLPADTPAALCR
CCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
RMADCVRAGLRPGATVAAVWPLPGEIDLRGLCAELHHGGYGVLLPETPPRGHPLRFRLWR
HHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEC
PGVPMQAGRFGTLHPDGPVARPDFVFVPLLGFDRRGFRLGYGGGYYDRTLAGLDSVPAAG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCC
YGLSAQEVPAVPVGRHDVPLPVIVTERETIRCGA
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]