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The map label for this gene is hmp [H]

Identifier: 162146318

GI number: 162146318

Start: 490175

End: 491395

Strand: Reverse

Name: hmp [H]

Synonym: GDI_0493

Alternate gene names: 162146318

Gene position: 491395-490175 (Counterclockwise)

Preceding gene: 162146320

Following gene: 162146317

Centisome position: 12.46

GC content: 69.12

Gene sequence:

>1221_bases
TTGGAGCGCATGATGGCTTCCCCCCTGGACGACAAGACCCGCGCCATCGTTTCCGCCTGCGTGCCGGCGCTGGAAGCGCA
TGGACTGGCGATCACGACCGAAATGTACCGGCGCCTGCTGGCCAACCCCGAGATCCGCGACCTGTTCAACCTGTCGCACC
AGAAGGATGGCGAGCAGCCCACGGCGCTGGCCCTGGCGGTGCTGGCCTATGCGCGCAACATCGACAATCCGGGCGCGCTG
GGCGGCATGGTCGAACGGATTGCCGAAAAGCATGTCGGACTGAATATCCGGGCGGAGCATTACCCGTTCGTGGCCGAGGC
CCTGCTGGGTGCGATCGCGCACGTGCTGGGCGACGCCGCCACGCCCGAGATCCTGGACGCATGGGGCAAGGCCTACTGGT
TCCTGGCCGACATCCTGATCGGGCGCGAGGCGCAGATCTATGACGCCCATGCGGCGGCGCCGGGCGGCTGGACGGGGTGG
CGCCCCTTCACCGTGCGCCGACGCACGCGCGAGAGCGAGACCGTGACGTCGTTCGAACTGGTGCCGGTCGATGGGGCGCC
GGTGATGCGCCACCGCCCCGGACAATATCTCAGCTTCCGGCTGGACGTGCCGGGCCTGGGGTCGCAACGGCGCAACTATA
GCATTTCGTCGAAACCGGATGACGGGTCCTACCGGATCAGCGTCCGGCGGATTCCGGGCGGCGCGGTGTCCGGCTGGCTG
CATGACGACGTGCGCGAGGGCACGGTCCTGCAGGTGTCCGCCCCCGCCGGCGATTTCACGCTGGCCGATCCCCTGCCGCC
CGCCATCGTGCTGCTGAGCGCCGGGGTGGGGCTGACGCCCATGATCTCGATGCTGGACGCGCTGGCAGCCCGGGCGGACG
CCCCGTCCATCCATTATATCCATGCCACGCGCACACCGGACACCGAGGCATTCGGACGGCACGTCCGGCGGCTGGCGGCG
GAGGGGCGGATTCGCGGCGACGTGTTCTACAGCCGCCGGGCGCCGGCGGACGGGGAGGACGCCGGAACCGCGCACCATAC
CGGGCGTATGACGATGGACTGGCTGCGTGCGAACCTGGCGGCGGATGCCACCTATTACGTCTGCGGCCCCGACGGTTTCA
TGCGCGATGCCGTTTCCACCCTGAAGGCGGCCGGCGTGCCGGCCGGACGGGTGCGCCATGAATTCTTCGGTTCGGCGCAT
GACGGGGCATTGGTGATGTGA

Upstream 100 bases:

>100_bases
TGAGGGCATAGTCGGTATGCAGGGTCAGGCGCATCGCTAAAAGATATCCATAATTGACATCTTTATCCAGCGGCGCAAAA
GATGCATCGTTTTTACATCT

Downstream 100 bases:

>100_bases
CACGCCCAGCCATACGTGTCCGGCGGATCTATGATCCGCCGGAGCCAGCGGAAGGCGCGCGCGTCCTGGTGGACCGGCTG
TGGCCGCGCGGGATGAGCAA

Product: flavohemoprotein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MERMMASPLDDKTRAIVSACVPALEAHGLAITTEMYRRLLANPEIRDLFNLSHQKDGEQPTALALAVLAYARNIDNPGAL
GGMVERIAEKHVGLNIRAEHYPFVAEALLGAIAHVLGDAATPEILDAWGKAYWFLADILIGREAQIYDAHAAAPGGWTGW
RPFTVRRRTRESETVTSFELVPVDGAPVMRHRPGQYLSFRLDVPGLGSQRRNYSISSKPDDGSYRISVRRIPGGAVSGWL
HDDVREGTVLQVSAPAGDFTLADPLPPAIVLLSAGVGLTPMISMLDALAARADAPSIHYIHATRTPDTEAFGRHVRRLAA
EGRIRGDVFYSRRAPADGEDAGTAHHTGRMTMDWLRANLAADATYYVCGPDGFMRDAVSTLKAAGVPAGRVRHEFFGSAH
DGALVM

Sequences:

>Translated_406_residues
MERMMASPLDDKTRAIVSACVPALEAHGLAITTEMYRRLLANPEIRDLFNLSHQKDGEQPTALALAVLAYARNIDNPGAL
GGMVERIAEKHVGLNIRAEHYPFVAEALLGAIAHVLGDAATPEILDAWGKAYWFLADILIGREAQIYDAHAAAPGGWTGW
RPFTVRRRTRESETVTSFELVPVDGAPVMRHRPGQYLSFRLDVPGLGSQRRNYSISSKPDDGSYRISVRRIPGGAVSGWL
HDDVREGTVLQVSAPAGDFTLADPLPPAIVLLSAGVGLTPMISMLDALAARADAPSIHYIHATRTPDTEAFGRHVRRLAA
EGRIRGDVFYSRRAPADGEDAGTAHHTGRMTMDWLRANLAADATYYVCGPDGFMRDAVSTLKAAGVPAGRVRHEFFGSAH
DGALVM
>Mature_406_residues
MERMMASPLDDKTRAIVSACVPALEAHGLAITTEMYRRLLANPEIRDLFNLSHQKDGEQPTALALAVLAYARNIDNPGAL
GGMVERIAEKHVGLNIRAEHYPFVAEALLGAIAHVLGDAATPEILDAWGKAYWFLADILIGREAQIYDAHAAAPGGWTGW
RPFTVRRRTRESETVTSFELVPVDGAPVMRHRPGQYLSFRLDVPGLGSQRRNYSISSKPDDGSYRISVRRIPGGAVSGWL
HDDVREGTVLQVSAPAGDFTLADPLPPAIVLLSAGVGLTPMISMLDALAARADAPSIHYIHATRTPDTEAFGRHVRRLAA
EGRIRGDVFYSRRAPADGEDAGTAHHTGRMTMDWLRANLAADATYYVCGPDGFMRDAVSTLKAAGVPAGRVRHEFFGSAH
DGALVM

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=395, Percent_Identity=41.0126582278481, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1787098, Length=257, Percent_Identity=30.7392996108949, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787658, Length=247, Percent_Identity=26.7206477732794, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321673, Length=408, Percent_Identity=34.5588235294118, Blast_Score=215, Evalue=1e-56,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43986; Mature: 43986

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMMASPLDDKTRAIVSACVPALEAHGLAITTEMYRRLLANPEIRDLFNLSHQKDGEQP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHCCHHCCCCCCH
TALALAVLAYARNIDNPGALGGMVERIAEKHVGLNIRAEHYPFVAEALLGAIAHVLGDAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCC
TPEILDAWGKAYWFLADILIGREAQIYDAHAAAPGGWTGWRPFTVRRRTRESETVTSFEL
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEE
VPVDGAPVMRHRPGQYLSFRLDVPGLGSQRRNYSISSKPDDGSYRISVRRIPGGAVSGWL
EECCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCH
HDDVREGTVLQVSAPAGDFTLADPLPPAIVLLSAGVGLTPMISMLDALAARADAPSIHYI
HHHCCCCEEEEEECCCCCCEECCCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCCCEEEE
HATRTPDTEAFGRHVRRLAAEGRIRGDVFYSRRAPADGEDAGTAHHTGRMTMDWLRANLA
EECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ADATYYVCGPDGFMRDAVSTLKAAGVPAGRVRHEFFGSAHDGALVM
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEC
>Mature Secondary Structure
MERMMASPLDDKTRAIVSACVPALEAHGLAITTEMYRRLLANPEIRDLFNLSHQKDGEQP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHCCHHCCCCCCH
TALALAVLAYARNIDNPGALGGMVERIAEKHVGLNIRAEHYPFVAEALLGAIAHVLGDAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCC
TPEILDAWGKAYWFLADILIGREAQIYDAHAAAPGGWTGWRPFTVRRRTRESETVTSFEL
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEE
VPVDGAPVMRHRPGQYLSFRLDVPGLGSQRRNYSISSKPDDGSYRISVRRIPGGAVSGWL
EECCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCH
HDDVREGTVLQVSAPAGDFTLADPLPPAIVLLSAGVGLTPMISMLDALAARADAPSIHYI
HHHCCCCEEEEEECCCCCCEECCCCCHHHHEEECCCCHHHHHHHHHHHHHHCCCCCEEEE
HATRTPDTEAFGRHVRRLAAEGRIRGDVFYSRRAPADGEDAGTAHHTGRMTMDWLRANLA
EECCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ADATYYVCGPDGFMRDAVSTLKAAGVPAGRVRHEFFGSAHDGALVM
CCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]