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The map label for this gene is nifK [H]

Identifier: 162146263

GI number: 162146263

Start: 436057

End: 437592

Strand: Direct

Name: nifK [H]

Synonym: GDI_0438

Alternate gene names: 162146263

Gene position: 436057-437592 (Clockwise)

Preceding gene: 162146262

Following gene: 162146264

Centisome position: 11.06

GC content: 60.94

Gene sequence:

>1536_bases
ATGCCTCAAAATGTCGATAAGATTCTCGATCACGCACCGTTGTTCCGCGAACCGGAATATCAGGAGATGCTGGCCGGGAA
AGCGAAGCTGGAGAACATGCCTCCGGCGGACAAGGTCGTCGAGATCGCCGACTGGACCAAGAGCTGGGAGTACCGCGAGA
AAAATTTCGCGCGCGAATCCCTGTCGGTCAATCCGGCCAAGGCCTGCCAGCCGCTGGGCGCCGTCTTCGTGGCCTCGGGT
TTCGAGCGCACCATGAGCTTCGTGCACGGCTCGCAGGGATGCGTCGCGTACTATCGTTCGCATCTCTCCCGCCATTTCAA
GGAGCCGTCATCGGCCGTGTCGTCGTCCATGACGGAAGACGCGGCGGTGTTCGGCGGCCTGAACAACATGGTGGACGGGC
TGGCGAATACCTACAAGCTGTATGACCCGAAGATGATCGCGGTGTCGACGACCTGCATGGCGGAGGTCATCGGCGACGAT
CTGCACGCCTTCATCCAGACGGCCAAGGGCAAGGGGTCCGTCCCCGAGGAATTTGACGTTCCGTTCGCCCATACCCCGGC
CTTCGTCGGCAGCCACGTGACGGGCTACGACAACATGCTCAAGGGTATCCTGGAGCATTTCTGGAAGGGCAGGACGCCGG
TCCCCAACCGTTCGGTCAACATCATCCCCGGGTTCGACGGTTTCGCGGTCGGCAACAATCGCGAGCTGAAGCGCATCCTG
GGCATGATGGGCGTGCAGTACACGATCCTGTCCGACGTGTCGGATCAGTTCGATACGCCGTCGGATGGCGAGTACCGCAT
GTATGACGGCGGCACGAAGATCGAGGCCGCGCGTGATGCGGTGAACGCCGATTACACGATTTCGCTTCAGGAATACTGCA
CGCCGAAGACGCTGGAATATTGCCAGAGCTTCGGGCAGAAGACCGCGTCCTTCCATTACCCGCTGGGCATCGGGGCGACG
GACGACCTGCTGCAGAAACTGTCGGAGATTTCGGGCAAGCCGGTTCCGCAGGAACTGGAAATGGAGCGTGGCCGCCTGGT
CGACGCGCTGGCCGACAGCCAGGCCTACCTGCATGGCAAGACGTACGCGATCTACGGCGATCCCGATTTCGTCTATGGCA
TGGCGCGCTTCATCCTGGAAACCGGGGGCGAGCCGAAGCACTGCCTGGCGACCAACGGCAGCAAGGCGTGGGAAGCGCAG
ATGCAGGAACTGTTCGACAGTTCCCCGTTCGGCGTCGGGTGCAAGGCGTGGGGCGGCAAGGATCTGTGGCATATGCGGTC
CCTGCTGGCGACGGAAAAGGTCGACCTGTTGATCGGCAATTCGTATGGCAAGTATCTGGAACGCGATACGGACACGCCGC
TGATCCGTCTGATGTTCCCGATCTTCGACCGGCATCATCACCATCGTTTCCCGGTCTGGGGATATCAGGGCGCCCTGCGC
GTTCTGGTGACGTTGCTGGACAAGATCTTCGACAAGCTTGACGACGATACGATCCAGGCGGGCGTCACCGATTATTCCTT
TGACCTGACGCGCTGA

Upstream 100 bases:

>100_bases
AGCATGTTCAAGGCTCCGTGGAAAAACGCCGCCTGAGGCCATAACGCAGCCGGCGTGCCGTATGGCGCACGCGGGTGGGT
AAAGGACAGAGGGTAAGATC

Downstream 100 bases:

>100_bases
ACACTACGCCGGGCGGTCCCGTGCCGCCCGGCGTCCTACATGATGCCGGATCCGAGGGTTGGAGAGGACGATGAGCGACG
CTTTGAAGGCGAAGGTTACC

Product: nitrogenase FeMo beta subunit protein; NifK

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARESLSVNPAKACQPLGAVFVASG
FERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDD
LHAFIQTAKGKGSVPEEFDVPFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRIL
GMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEYCQSFGQKTASFHYPLGIGAT
DDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGKTYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQ
MQELFDSSPFGVGCKAWGGKDLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALR
VLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR

Sequences:

>Translated_511_residues
MPQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARESLSVNPAKACQPLGAVFVASG
FERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDD
LHAFIQTAKGKGSVPEEFDVPFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRIL
GMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEYCQSFGQKTASFHYPLGIGAT
DDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGKTYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQ
MQELFDSSPFGVGCKAWGGKDLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALR
VLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR
>Mature_510_residues
PQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARESLSVNPAKACQPLGAVFVASGF
ERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDL
HAFIQTAKGKGSVPEEFDVPFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRILG
MMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEYCQSFGQKTASFHYPLGIGATD
DLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGKTYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQM
QELFDSSPFGVGCKAWGGKDLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALRV
LVTLLDKIFDKLDDDTIQAGVTDYSFDLTR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 57028; Mature: 56897

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARES
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEHHHHHCCHHHHHHHHHHHH
LSVNPAKACQPLGAVFVASGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTED
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLHAFIQTAKGKGSVPEEFDV
HHHHCCHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRIL
CCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCHHHHHHH
GMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEY
HHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEHHHHCCHHHHHH
CQSFGQKTASFHYPLGIGATDDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGK
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCC
TYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQMQELFDSSPFGVGCKAWGGK
EEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALR
HHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR
HHHHHHHHHHHHCCCCHHHCCCCCCEEECCC
>Mature Secondary Structure 
PQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARES
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEHHHHHCCHHHHHHHHHHHH
LSVNPAKACQPLGAVFVASGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTED
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLHAFIQTAKGKGSVPEEFDV
HHHHCCHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRIL
CCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCHHHHHHH
GMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEY
HHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEHHHHCCHHHHHH
CQSFGQKTASFHYPLGIGATDDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGK
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCC
TYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQMQELFDSSPFGVGCKAWGGK
EEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALR
HHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR
HHHHHHHHHHHHCCCCHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]