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The map label for this gene is nifE [H]

Identifier: 162146264

GI number: 162146264

Start: 437663

End: 439102

Strand: Direct

Name: nifE [H]

Synonym: GDI_0439

Alternate gene names: 162146264

Gene position: 437663-439102 (Clockwise)

Preceding gene: 162146263

Following gene: 162146265

Centisome position: 11.1

GC content: 65.14

Gene sequence:

>1440_bases
ATGAGCGACGCTTTGAAGGCGAAGGTTACCGAACTGTTCAACGAGCCAGGGTGTGAAAAGAACCTGGCGAAGGGCGAAAA
GGAGCGCAGGAAGGGGTGTTCCAAGCCGCTGACGCCCGGTGCGGCGGCGGGGGGGTGCGCGTTTGACGGCGCGAAGATCG
CGCTGCAGCCCATTACGGATGCGGTCCATCTGGTCCATGGTCCGCTGGCCTGCGAAGGCAATGGCTGGGACAACCGGCAT
GCCGCCAGTTCCGGACCGAAACTGTATCGCCTGGGCGCCACGACGGATCTGTCGCAGATGGATATCGTCATGGGGCTGGG
CGAAAAGCGGCTGTACAAGGCCATCAAGGACGTCATCGCCCGCTATGCGCCACCCGCGGTCTTCGTCTATTCCACATGCG
TTCCGGCGCTGACCGGCGATGATGTCGTCGCGGTCTGCGCCCATGCCAGCCAGAAGCTGGCGACGCCGTGCATCCCGGTC
AACGCCCCGGGCTTTGTCGGCGGGAAGAACCTGGGCAACAAGCTGGCGGGCGAGGCGCTGCTGGATTATGTGATCGGGAC
GATGGAGCCCGAAATCAGCACGCCCACGGATATCAATATTCTTGGCGAATATAATCTTTCGGGCGAATTGTGGCAGATCC
TGCCGCTGTTTCGGGCCCTGGGGATTCGGGTCCATGCCTGCGTGACGGGCGATGCCCGCTATCGCGAGGTTGCGTCGGCG
CACCGGTCGCGCGTCAACATGATGGTGTGCTCGACCTCGCTGATCAACGTCGCGCGCAAGATGGAAGAACGGTGGGGCAT
TCCGTACTTTGAAGGATCTTTCTACGGAATCGAGGACACCTCCGCCGCCCTGCGGGCCATCGCCGGGATGCTGGTGGCCC
GTGGCGCGCCGGCCGACCTGCCGGCCCGGGCCGAGGCCCTGATCGCGGAGGAGGAAGCCCGCGCCTGGGAGGCCATCGCC
CCCTATCGCGCGCGGCTGGAGGGCAAGCGGGTGCTGCTCTATACCGGCGGTGTCAAATCCTGGTCGATCGTTTCCGCCCT
GCAGGAGATCGGGATGGTCGTCGTGGGCACGTCGGTCCGGAAATCCACCGATAACGACAAGCAGAAGATCAAGGACCTGA
TGGGCGGCGACGCCCACATGGTGGACGCCATCCCGCCGCGGGAGATGTACGCCCAGTTGCGGCGCGGCGACGCGGACATC
CTGCTGTCCGGCGGACGGACGCAGTTCGTGGCGCTGAAGGCCCGGGTGCCCTGGCTGGATATCAACCAGGAACGGCACCA
GGCCTATGCCGGATATGACGGCATGGTGGCGCTGGTGCGTGAACTGGATCGGTCCCTGTCCAACCCGGTCTGGGCGGATG
TCCGCCGCCCGGCCCCGTGGGAGGAGGACGATGCCGATGCGTTCCTGGATGACGCGCCGTTCCTGACCCCCTTGTCCTGA

Upstream 100 bases:

>100_bases
ACCGATTATTCCTTTGACCTGACGCGCTGAACACTACGCCGGGCGGTCCCGTGCCGCCCGGCGTCCTACATGATGCCGGA
TCCGAGGGTTGGAGAGGACG

Downstream 100 bases:

>100_bases
GGAGTTCGTCCGTGGCCACCATCGTGAAGCCGCGCAAGGCGGCGTCCGTCAATCCGCTGAAATCCTCGACGCCGCTGGGC
GCGGCGCTGGCCTATCTGGG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSDALKAKVTELFNEPGCEKNLAKGEKERRKGCSKPLTPGAAAGGCAFDGAKIALQPITDAVHLVHGPLACEGNGWDNRH
AASSGPKLYRLGATTDLSQMDIVMGLGEKRLYKAIKDVIARYAPPAVFVYSTCVPALTGDDVVAVCAHASQKLATPCIPV
NAPGFVGGKNLGNKLAGEALLDYVIGTMEPEISTPTDINILGEYNLSGELWQILPLFRALGIRVHACVTGDARYREVASA
HRSRVNMMVCSTSLINVARKMEERWGIPYFEGSFYGIEDTSAALRAIAGMLVARGAPADLPARAEALIAEEEARAWEAIA
PYRARLEGKRVLLYTGGVKSWSIVSALQEIGMVVVGTSVRKSTDNDKQKIKDLMGGDAHMVDAIPPREMYAQLRRGDADI
LLSGGRTQFVALKARVPWLDINQERHQAYAGYDGMVALVRELDRSLSNPVWADVRRPAPWEEDDADAFLDDAPFLTPLS

Sequences:

>Translated_479_residues
MSDALKAKVTELFNEPGCEKNLAKGEKERRKGCSKPLTPGAAAGGCAFDGAKIALQPITDAVHLVHGPLACEGNGWDNRH
AASSGPKLYRLGATTDLSQMDIVMGLGEKRLYKAIKDVIARYAPPAVFVYSTCVPALTGDDVVAVCAHASQKLATPCIPV
NAPGFVGGKNLGNKLAGEALLDYVIGTMEPEISTPTDINILGEYNLSGELWQILPLFRALGIRVHACVTGDARYREVASA
HRSRVNMMVCSTSLINVARKMEERWGIPYFEGSFYGIEDTSAALRAIAGMLVARGAPADLPARAEALIAEEEARAWEAIA
PYRARLEGKRVLLYTGGVKSWSIVSALQEIGMVVVGTSVRKSTDNDKQKIKDLMGGDAHMVDAIPPREMYAQLRRGDADI
LLSGGRTQFVALKARVPWLDINQERHQAYAGYDGMVALVRELDRSLSNPVWADVRRPAPWEEDDADAFLDDAPFLTPLS
>Mature_478_residues
SDALKAKVTELFNEPGCEKNLAKGEKERRKGCSKPLTPGAAAGGCAFDGAKIALQPITDAVHLVHGPLACEGNGWDNRHA
ASSGPKLYRLGATTDLSQMDIVMGLGEKRLYKAIKDVIARYAPPAVFVYSTCVPALTGDDVVAVCAHASQKLATPCIPVN
APGFVGGKNLGNKLAGEALLDYVIGTMEPEISTPTDINILGEYNLSGELWQILPLFRALGIRVHACVTGDARYREVASAH
RSRVNMMVCSTSLINVARKMEERWGIPYFEGSFYGIEDTSAALRAIAGMLVARGAPADLPARAEALIAEEEARAWEAIAP
YRARLEGKRVLLYTGGVKSWSIVSALQEIGMVVVGTSVRKSTDNDKQKIKDLMGGDAHMVDAIPPREMYAQLRRGDADIL
LSGGRTQFVALKARVPWLDINQERHQAYAGYDGMVALVRELDRSLSNPVWADVRRPAPWEEDDADAFLDDAPFLTPLS

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 51802; Mature: 51671

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00334 MYB_2 ; PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALKAKVTELFNEPGCEKNLAKGEKERRKGCSKPLTPGAAAGGCAFDGAKIALQPITD
CCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
AVHLVHGPLACEGNGWDNRHAASSGPKLYRLGATTDLSQMDIVMGLGEKRLYKAIKDVIA
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHH
RYAPPAVFVYSTCVPALTGDDVVAVCAHASQKLATPCIPVNAPGFVGGKNLGNKLAGEAL
HHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
LDYVIGTMEPEISTPTDINILGEYNLSGELWQILPLFRALGIRVHACVTGDARYREVASA
HHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
HRSRVNMMVCSTSLINVARKMEERWGIPYFEGSFYGIEDTSAALRAIAGMLVARGAPADL
HHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
PARAEALIAEEEARAWEAIAPYRARLEGKRVLLYTGGVKSWSIVSALQEIGMVVVGTSVR
CCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEECCHH
KSTDNDKQKIKDLMGGDAHMVDAIPPREMYAQLRRGDADILLSGGRTQFVALKARVPWLD
CCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEE
INQERHQAYAGYDGMVALVRELDRSLSNPVWADVRRPAPWEEDDADAFLDDAPFLTPLS
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SDALKAKVTELFNEPGCEKNLAKGEKERRKGCSKPLTPGAAAGGCAFDGAKIALQPITD
CCHHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHH
AVHLVHGPLACEGNGWDNRHAASSGPKLYRLGATTDLSQMDIVMGLGEKRLYKAIKDVIA
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHH
RYAPPAVFVYSTCVPALTGDDVVAVCAHASQKLATPCIPVNAPGFVGGKNLGNKLAGEAL
HHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
LDYVIGTMEPEISTPTDINILGEYNLSGELWQILPLFRALGIRVHACVTGDARYREVASA
HHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
HRSRVNMMVCSTSLINVARKMEERWGIPYFEGSFYGIEDTSAALRAIAGMLVARGAPADL
HHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
PARAEALIAEEEARAWEAIAPYRARLEGKRVLLYTGGVKSWSIVSALQEIGMVVVGTSVR
CCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEECCHH
KSTDNDKQKIKDLMGGDAHMVDAIPPREMYAQLRRGDADILLSGGRTQFVALKARVPWLD
CCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEE
INQERHQAYAGYDGMVALVRELDRSLSNPVWADVRRPAPWEEDDADAFLDDAPFLTPLS
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]