Click here to switch to the map view.

The map label for this gene is nifD [H]

Identifier: 162146262

GI number: 162146262

Start: 434493

End: 435992

Strand: Direct

Name: nifD [H]

Synonym: GDI_0437

Alternate gene names: 162146262

Gene position: 434493-435992 (Clockwise)

Preceding gene: 162146261

Following gene: 162146263

Centisome position: 11.02

GC content: 59.07

Gene sequence:

>1500_bases
ATGAGTCTGGATGAAGACAAGACCAACGATAGCGCGTTTCACGCTCGCCTGATCGCGGAAGTGCTGGAAGCATACCCGGA
CAAGGCCCGCAAGCGCCGGCAGAAGCATCTGAACGTCGCCGGCCAAGCCGAAGCCGAGGCGCAGGACGCCGGCGAAGAAG
GCGTGATGCTGAGCGAATGCGACGTCAAGTCGAACGTGAAGTCCGTGCCGGGCGTCATGACGATCCGCGGCTGCGCCTAT
GCCGGATCGAAGGGCGTGGTCTGGGGGCCGGTCAAGGACATGGTCCATATCAGCCATGGCCCGGTCGGCTGCGGACAGTA
TTCGTGGTCGCAGCGCCGCAACTACTATATCGGCAATACCGGCGTCGACTCGTTCGTCACCATGCAGTTCACGTCCGATT
TCCAGGAAAAGGACATCGTGTTCGGCGGTGACAAGAAGCTGGAAAAGATCATCGACGAAATCGATGAGCTCTTTCCGCTG
GCCAAGGGAATTTCGGTGCAGTCCGAATGCCCGATCGGCCTGATCGGCGACGACATCGAGGCGGTATCGCGCAAGAAGAA
GAAGGAAATCGGCAAGACCATCGTGCCGGTCCGGTGCGAGGGCTTCCGCGGCGTTTCGCAGTCGCTGGGCCATCATATCG
CCAATGATGCCATCCGGGACTGGGTGTTCGACGGCGAGGACAAGCACGCGGCGTTCGAGACGACGCCTTATGACGTCAAC
GTCATCGGTGACTACAACATCGGCGGCGATGCCTGGTCGTCACGCATCCTGCTGGAAGAAATGGGCCTGCGCGTCGTGGG
CAACTGGTCCGGCGATGCGACGCTGGCCGAAATCGAGCGCGCGCCCAAGGCGAAGCTGAACCTGATTCACTGCTATCGCT
CGATGAACTATATCTGCCGGCATATGGAAGAAAAGTACAATATTCCCTGGACGGAATATAACTTCTTCGGCCCGTCGCAG
ATCGCGGCATCGCTGCGCAAGATCGCGGCCCTGTTCGACGAGAAGATCCAGGAAGGCGCCGAGCGCGTCATCGCGAAATA
CCAGCCCCTGGTCGATGCGGTGATCGAGAAGTTCCGTCCGCGCCTGGCGGGCAAGAAGGTCATGCTGTATGTCGGCGGCC
TGCGTCCGCGCCACGTCGTCAATGCCTATAACGACCTGGGCATGGAAATCGTCGGCACTGGCTACGAATTCGGCCACAAC
GACGACTATCAGCGCACTGGACACTACGTCAGGGAAGGCACGCTGATCTACGACGACGTCACCGGGTATGAACTGGAGAA
GTTCATCGAAGGCATCCGTCCGGACCTGGTCGGATCGGGCATCAAGGAAAAATATCCCGTGCAGAAGATGGGCATCCCGT
TCCGTCAGATGCATTCATGGGATTATTCGGGCCCGTATCATGGCTATGATGGTTTCGCCATCTTCGCCCGTGACATGGAT
CTTGCCATCAATAATCCGGTCTGGAGCATGTTCAAGGCTCCGTGGAAAAACGCCGCCTGA

Upstream 100 bases:

>100_bases
CCAAGGAGGCCAAGGCCGCGGCAGCGCTGGCCTGATGCCTGAATGTCCGGCGGGTAAGCCGCCGGGCATTGTTTCCCACT
TCCACGAAGGGGACCAGAAA

Downstream 100 bases:

>100_bases
GGCCATAACGCAGCCGGCGTGCCGTATGGCGCACGCGGGTGGGTAAAGGACAGAGGGTAAGATCATGCCTCAAAATGTCG
ATAAGATTCTCGATCACGCA

Product: FeMo protein of nitrogenase alpha subunit; NifD

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSECDVKSNVKSVPGVMTIRGCAY
AGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNTGVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPL
AKGISVQSECPIGLIGDDIEAVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVN
VIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYNIPWTEYNFFGPSQ
IAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRPRLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHN
DDYQRTGHYVREGTLIYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMD
LAINNPVWSMFKAPWKNAA

Sequences:

>Translated_499_residues
MSLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSECDVKSNVKSVPGVMTIRGCAY
AGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNTGVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPL
AKGISVQSECPIGLIGDDIEAVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVN
VIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYNIPWTEYNFFGPSQ
IAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRPRLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHN
DDYQRTGHYVREGTLIYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMD
LAINNPVWSMFKAPWKNAA
>Mature_498_residues
SLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSECDVKSNVKSVPGVMTIRGCAYA
GSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNTGVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPLA
KGISVQSECPIGLIGDDIEAVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVNV
IGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYNIPWTEYNFFGPSQI
AASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRPRLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHND
DYQRTGHYVREGTLIYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDL
AINNPVWSMFKAPWKNAA

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56021; Mature: 55890

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSEC
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEECC
DVKSNVKSVPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNT
CCHHHHHHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEECCC
GVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPLAKGISVQSECPIGLIGDDIE
CCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHH
AVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVN
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEE
VIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICR
EEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
HMEEKYNIPWTEYNFFGPSQIAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHNDDYQRTGHYVREGTLIYDDV
HHCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCEEEEECC
TGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMD
CCHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCC
LAINNPVWSMFKAPWKNAA
EEECCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSEC
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEECC
DVKSNVKSVPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNT
CCHHHHHHCCCEEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEECCC
GVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPLAKGISVQSECPIGLIGDDIE
CCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHH
AVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVN
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEE
VIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICR
EEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
HMEEKYNIPWTEYNFFGPSQIAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHNDDYQRTGHYVREGTLIYDDV
HHCCCEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCEEEEECC
TGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMD
CCHHHHHHHHHCCHHHHCCCCCCCCCHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCC
LAINNPVWSMFKAPWKNAA
EEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]