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The map label for this gene is nifH [H]

Identifier: 162146261

GI number: 162146261

Start: 433531

End: 434427

Strand: Direct

Name: nifH [H]

Synonym: GDI_0436

Alternate gene names: 162146261

Gene position: 433531-434427 (Clockwise)

Preceding gene: 162146260

Following gene: 162146262

Centisome position: 10.99

GC content: 62.54

Gene sequence:

>897_bases
ATGAGCAAGCTTCGCCAAATCGCCTTTTATGGAAAGGGAGGAATCGGCAAGTCGACGACCTCCCAGAATACCCTCGCCGC
ACTGGTCGAGATGGGCCAGAAGATCCTGATCGTCGGCTGCGATCCGAAGGCGGATTCCACCCGCCTGATCCTGAACGCCA
AGGCGCAGGATACGGTCCTGAGCCTGGCAGCGGAAGCCGGATCGGTCGAGGACCTGGAACTCGAGGACGTGCTGAAGATC
GGCTACAAGGGCATCAAGTGCGTCGAATCCGGCGGGCCGGAGCCCGGCGTCGGCTGCGCGGGACGCGGCGTGATCACCTC
GATCAACTTCCTCGAGGAAAACGGCGCCTATGACGATGTCGACTACGTCTCCTACGACGTGCTGGGCGACGTGGTCTGCG
GCGGCTTCGCCATGCCGATCCGCGAAAACAAGGCCCAGGAAATCTACATCGTCATGTCGGGCGAGATGATGGCCCTGTAC
GCGGCCAACAACATCGCCAAGGGCATTCTGAAATACGCCCATTCCGGCGGCGTGCGCCTGGGTGGACTGATCTGCAACGA
ACGTCAGACCGACCGCGAATACGATCTGGCGGACGCGCTGGCCAAGCGGCTGAATTCCAAGCTGGTGCATTTCGTGCCGC
GTGCCAACATCGTCCAGCACGCGGAACTGCGCAAGCAGACGGTGATCGAGTACGCGCCGGATTCCGCGCAGGCCGGGGAA
TACCGGACGCTGGCGCAGAAGATCCATGCGAATTCCGGGCAGGGCACGGTGCCCACGCCGATCACGATGGAAGAGCTGGA
AGACATGCTGCTCGAGTTCGGCATCATGAAGACCGATGAACAAGCCCTGGCCGAACTGGCCGCCAAGGAGGCCAAGGCCG
CGGCAGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
AAGATCCCCGATCCGGCGTGTGGCATGGCGTTTGCGGATGTCCTGTGTGTCGGGCCTGGCGGCCCGCGCGATGCGATCTA
TCGGAAAACAGGACGCCATC

Downstream 100 bases:

>100_bases
TGCCTGAATGTCCGGCGGGTAAGCCGCCGGGCATTGTTTCCCACTTCCACGAAGGGGACCAGAAAATGAGTCTGGATGAA
GACAAGACCAACGATAGCGC

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSKLRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNAKAQDTVLSLAAEAGSVEDLELEDVLKI
GYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDREYDLADALAKRLNSKLVHFVPRANIVQHAELRKQTVIEYAPDSAQAGE
YRTLAQKIHANSGQGTVPTPITMEELEDMLLEFGIMKTDEQALAELAAKEAKAAAALA

Sequences:

>Translated_298_residues
MSKLRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNAKAQDTVLSLAAEAGSVEDLELEDVLKI
GYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHSGGVRLGGLICNERQTDREYDLADALAKRLNSKLVHFVPRANIVQHAELRKQTVIEYAPDSAQAGE
YRTLAQKIHANSGQGTVPTPITMEELEDMLLEFGIMKTDEQALAELAAKEAKAAAALA
>Mature_297_residues
SKLRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNAKAQDTVLSLAAEAGSVEDLELEDVLKIG
YKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
ANNIAKGILKYAHSGGVRLGGLICNERQTDREYDLADALAKRLNSKLVHFVPRANIVQHAELRKQTVIEYAPDSAQAGEY
RTLAQKIHANSGQGTVPTPITMEELEDMLLEFGIMKTDEQALAELAAKEAKAAAALA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31874; Mature: 31743

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNAKAQDTVL
CCCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHH
SLAAEAGSVEDLELEDVLKIGYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDV
HHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHEEEEEHHCCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCEE
GGLICNERQTDREYDLADALAKRLNSKLVHFVPRANIVQHAELRKQTVIEYAPDSAQAGE
EEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
YRTLAQKIHANSGQGTVPTPITMEELEDMLLEFGIMKTDEQALAELAAKEAKAAAALA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNAKAQDTVL
CCHHEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHH
SLAAEAGSVEDLELEDVLKIGYKGIKCVESGGPEPGVGCAGRGVITSINFLEENGAYDDV
HHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHEEEEEHHCCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCEE
GGLICNERQTDREYDLADALAKRLNSKLVHFVPRANIVQHAELRKQTVIEYAPDSAQAGE
EEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
YRTLAQKIHANSGQGTVPTPITMEELEDMLLEFGIMKTDEQALAELAAKEAKAAAALA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA