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The map label for this gene is nifB [H]

Identifier: 162146255

GI number: 162146255

Start: 429434

End: 430906

Strand: Direct

Name: nifB [H]

Synonym: GDI_0430

Alternate gene names: 162146255

Gene position: 429434-430906 (Clockwise)

Preceding gene: 162146254

Following gene: 162146256

Centisome position: 10.89

GC content: 62.86

Gene sequence:

>1473_bases
ATGGCACAGCTTATCAGTCTGGATACGCTTGCGACGGGAGGGGTCGAGCAGATGCGGTCGGCCCTGGAAGAGGGCGGGTG
TTCATCATCCAGTTGCGGCAGTTCCGAGGGGCCGGCCGACATGGCCCCGGAAATCTGGGAAAAGGTCAAGGACCATCCCT
GTTATTCGGAAGAAGCGCACCATTATTTCGCCCGCATGCATGTGGCGGTGGCGCCGGCGTGCAACATCCAGTGCAATTAC
TGCAACCGCAAATATGACTGCGCGAACGAAAGCCGCCCGGGGGTGGTGTCGGAAAAGCTGACGCCGGTGCAGGCGCGGCG
CAAGGTGATTTCGGTCGCCAACTCGGTGCCGCAGTTTTCGGTCCTGGGCATCGCGGGTCCAGGCGATGCCTGTTACGACT
GGAAGAAGACACGCGAAACGTTCGAACTGATCGCGAACGACATTCCCGATGTCAAATTGTGCCTGTCGACCAACGGCCTG
TCGCTTCCGGACCGCGTCGATGAGATCGCACGGATGAATATCGACCACGTCACGATCACCATCAACATGGTCGATCCCGA
GATCGGTACGAAGATCTATCCGTGGATCTTCTACCAGAACCGCCGCTGGACCGGGCTGGAGGCATCTCGGATCCTGCATG
AACGCCAGATGCTGGGGCTGGAAATGCTGGTGGCGCGCAACATTCTGGTGAAGGTCAATTCCGTCATGATCCCCGGGATC
AATGACGAACACCTGATCGAGGTGAACCGGGTCGTCAAAGGCAAGGGTGCCTTCCTGCACAATATCATGCCGCTGATTTC
CGATCCCGCGCATGGCACGCATTTCGGACTGACGGGCCAGCGTGGACCCAGGGCCGCGGAACTGAAGGCGTTGCAGGACC
GCCTGTCGGACGGTACGAAGCTGATGCGCCATTGCCGCCAGTGCCGCGCCGACGCGGTGGGACTGCTGGGCGAGGATCGC
GGGCAGGAATTCACCATGGACCAGGTGGCCGAGGAGGTCACCTACGACCCATCGCTGCGCGAAACCTACCGCGAGGTCGT
GGCGCAGGAGCGTGGCGAACACATGAACCAGCGCAGGGCCGCCGACGCCGCCCTGGCCGACATGACGTCCGAAGCGCCGG
TCCTGGTCGCCGTGGCGACCAAGGGCGGCGGCCGCATCAACCAGCATTTCGGCCATGCCCGGGAATTCCAGGTCTACGAG
GTCGGGGCGTCGGGCATTTCCTTCGTCGGGCACCGCAAGAGCGAGACCTATTGCGAAGGCGGCTGGGGCGAGGACGCGAC
GCTGGACGGTATCATCGCCACGCTGGAAGGCGTGGATGCCGTGCTGTGCGCCAAGGTCGGGGACTGCCCGCGCGATGCGA
TGCGCGCGGCGGGCATCGTGGTCAGCGACGAATACGCCCATGACTGGATCGAGGCCGGAATCACCGGATGGTACGCCGCG
TCCCTCGGTACGGAAATCCGTCAAACTGCGTGA

Upstream 100 bases:

>100_bases
TGTCACCCGCGTGGCGGCAGGCCATGACAGGACACACGGTTGGCGTTGGCATGGGTCTTGCGTCGGACCTGACGGCAAAA
GTCAGTCCGGGGAGTGGCCG

Downstream 100 bases:

>100_bases
AGACGGCAACATTTTTTCAGAAATCAGGAGTCTGACATGGCCTACAGGATCGTTACCTCCCAGTGCACCGTCTGCGGTGC
GTGTGAATTCGAATGCCCGA

Product: FeMo cofactor biosynthesis protein nifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAQLISLDTLATGGVEQMRSALEEGGCSSSSCGSSEGPADMAPEIWEKVKDHPCYSEEAHHYFARMHVAVAPACNIQCNY
CNRKYDCANESRPGVVSEKLTPVQARRKVISVANSVPQFSVLGIAGPGDACYDWKKTRETFELIANDIPDVKLCLSTNGL
SLPDRVDEIARMNIDHVTITINMVDPEIGTKIYPWIFYQNRRWTGLEASRILHERQMLGLEMLVARNILVKVNSVMIPGI
NDEHLIEVNRVVKGKGAFLHNIMPLISDPAHGTHFGLTGQRGPRAAELKALQDRLSDGTKLMRHCRQCRADAVGLLGEDR
GQEFTMDQVAEEVTYDPSLRETYREVVAQERGEHMNQRRAADAALADMTSEAPVLVAVATKGGGRINQHFGHAREFQVYE
VGASGISFVGHRKSETYCEGGWGEDATLDGIIATLEGVDAVLCAKVGDCPRDAMRAAGIVVSDEYAHDWIEAGITGWYAA
SLGTEIRQTA

Sequences:

>Translated_490_residues
MAQLISLDTLATGGVEQMRSALEEGGCSSSSCGSSEGPADMAPEIWEKVKDHPCYSEEAHHYFARMHVAVAPACNIQCNY
CNRKYDCANESRPGVVSEKLTPVQARRKVISVANSVPQFSVLGIAGPGDACYDWKKTRETFELIANDIPDVKLCLSTNGL
SLPDRVDEIARMNIDHVTITINMVDPEIGTKIYPWIFYQNRRWTGLEASRILHERQMLGLEMLVARNILVKVNSVMIPGI
NDEHLIEVNRVVKGKGAFLHNIMPLISDPAHGTHFGLTGQRGPRAAELKALQDRLSDGTKLMRHCRQCRADAVGLLGEDR
GQEFTMDQVAEEVTYDPSLRETYREVVAQERGEHMNQRRAADAALADMTSEAPVLVAVATKGGGRINQHFGHAREFQVYE
VGASGISFVGHRKSETYCEGGWGEDATLDGIIATLEGVDAVLCAKVGDCPRDAMRAAGIVVSDEYAHDWIEAGITGWYAA
SLGTEIRQTA
>Mature_489_residues
AQLISLDTLATGGVEQMRSALEEGGCSSSSCGSSEGPADMAPEIWEKVKDHPCYSEEAHHYFARMHVAVAPACNIQCNYC
NRKYDCANESRPGVVSEKLTPVQARRKVISVANSVPQFSVLGIAGPGDACYDWKKTRETFELIANDIPDVKLCLSTNGLS
LPDRVDEIARMNIDHVTITINMVDPEIGTKIYPWIFYQNRRWTGLEASRILHERQMLGLEMLVARNILVKVNSVMIPGIN
DEHLIEVNRVVKGKGAFLHNIMPLISDPAHGTHFGLTGQRGPRAAELKALQDRLSDGTKLMRHCRQCRADAVGLLGEDRG
QEFTMDQVAEEVTYDPSLRETYREVVAQERGEHMNQRRAADAALADMTSEAPVLVAVATKGGGRINQHFGHAREFQVYEV
GASGISFVGHRKSETYCEGGWGEDATLDGIIATLEGVDAVLCAKVGDCPRDAMRAAGIVVSDEYAHDWIEAGITGWYAAS
LGTEIRQTA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53742; Mature: 53611

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLISLDTLATGGVEQMRSALEEGGCSSSSCGSSEGPADMAPEIWEKVKDHPCYSEEAH
CCCEEECHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHH
HYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPVQARRKVISVANSVPQFS
HHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEE
VLGIAGPGDACYDWKKTRETFELIANDIPDVKLCLSTNGLSLPDRVDEIARMNIDHVTIT
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEE
INMVDPEIGTKIYPWIFYQNRRWTGLEASRILHERQMLGLEMLVARNILVKVNSVMIPGI
EEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCC
NDEHLIEVNRVVKGKGAFLHNIMPLISDPAHGTHFGLTGQRGPRAAELKALQDRLSDGTK
CCCHHEEEHHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCHHH
LMRHCRQCRADAVGLLGEDRGQEFTMDQVAEEVTYDPSLRETYREVVAQERGEHMNQRRA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADAALADMTSEAPVLVAVATKGGGRINQHFGHAREFQVYEVGASGISFVGHRKSETYCEG
HHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCC
GWGEDATLDGIIATLEGVDAVLCAKVGDCPRDAMRAAGIVVSDEYAHDWIEAGITGWYAA
CCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCEEEECHHHHHHHHHCCCHHHHH
SLGTEIRQTA
HHCCHHHHCC
>Mature Secondary Structure 
AQLISLDTLATGGVEQMRSALEEGGCSSSSCGSSEGPADMAPEIWEKVKDHPCYSEEAH
CCEEECHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHH
HYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPVQARRKVISVANSVPQFS
HHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEE
VLGIAGPGDACYDWKKTRETFELIANDIPDVKLCLSTNGLSLPDRVDEIARMNIDHVTIT
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEE
INMVDPEIGTKIYPWIFYQNRRWTGLEASRILHERQMLGLEMLVARNILVKVNSVMIPGI
EEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCC
NDEHLIEVNRVVKGKGAFLHNIMPLISDPAHGTHFGLTGQRGPRAAELKALQDRLSDGTK
CCCHHEEEHHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCHHH
LMRHCRQCRADAVGLLGEDRGQEFTMDQVAEEVTYDPSLRETYREVVAQERGEHMNQRRA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ADAALADMTSEAPVLVAVATKGGGRINQHFGHAREFQVYEVGASGISFVGHRKSETYCEG
HHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCC
GWGEDATLDGIIATLEGVDAVLCAKVGDCPRDAMRAAGIVVSDEYAHDWIEAGITGWYAA
CCCCCCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCEEEECHHHHHHHHHCCCHHHHH
SLGTEIRQTA
HHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]