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The map label for this gene is nifA [H]

Identifier: 162146254

GI number: 162146254

Start: 427533

End: 429278

Strand: Direct

Name: nifA [H]

Synonym: GDI_0429

Alternate gene names: 162146254

Gene position: 427533-429278 (Clockwise)

Preceding gene: 162146253

Following gene: 162146255

Centisome position: 10.84

GC content: 66.49

Gene sequence:

>1746_bases
ATGCCTACGGATACCGTTCCCTTTCGCCCCGTCAGCGGGGAAGGCCATTCGACCTGGGCTGAACGCGCCTTGTTCGGCCT
TCACGAGATATCGAAAATCCTGTGCGCGCCGACGGAGACCGTCGGAATCATCAGGAATGTCCTGGCCGTTCTGGAAAGCT
TCGTCGACCTGAACAATGCCGTCGTCGCGCTGTTCGACGCGGCGGGCAATGTGGAGACCATCATCGGCACCGAGGCCGAT
GACGCCGCCGCCCGGCGCTATTTCGATTCGATTCCCGAACAGGCGGTGGGACAGATCGCCGTCAGCCGCCAGCCGCTGCT
GGTGCCCGACGTAACGGGTGACACGCGCTATGGCTTCGCGACGTCGCAGGCCTGGACGGTGGGGGCGGGGCGCATCGGCG
TGATCGGCGTGCCGATCCTCAGCCGCGACAGGCTGGTGGGTGTCATGCTGCTCGATCGCCCGGCCATTCCGGAGGAATCG
GTCGTCGGCAGCATGGATGTCTGCCTCCTGTCGATGGTGGCGGGGCTGATGGGGCAGATGGTGCGCCTGCATCGGCTGGT
GCAGCGGGACCGGGAAAACCTGCTGCACGACAGCGGCCTCGCCCAGCCGGCGCCGCCGGTTGCCGATGGCGGCGGATATA
TGGGGATCGTCGGCGACAGTCCGGCGCTGCGGGCCGTTCTGCAGAAGGTCGAAATCGTCGCCAGAAGCGACGTCACCGTC
CTGTTGCGCGGTGAATCGGGCACGGGCAAGGAATTGTTCGCCCAGGCGATCCACCAGGCCTCGCCCCGGCATCGCAAGCC
GTTCGTCAAGCTGAACTGCGCGGCCCTGCCGGAAAGCGTTCTGGAATCCGAACTGTTCGGGCATGAAAAGGGCGCCTTTA
CCGGCGCGATCGGCCAGCGCAAGGGCCGGTTCGAACTGGCCGATGGCGGCACCCTGTTCATGGACGAGATCGGCGAGATC
TCGCCCAGTTTCCAGGCCAAGCTGCTGCGGGTGCTGCAGGAGGGCGAGTTCGAACGCGTGGGCGGCACCAGGACGCTGAA
GGTCGACGTGCGCGTGGTGACGGCGACAAATCGCAATCTGGAGGAAGCGGTCAGCAAGGGCCGCTTTCGCGCCGACCTCT
ATTACCGGCTGAGCGTCATTCCGATCTTCCTGCCGCCGCTGCGGGATCGCCCGACGGATATTCCCATGCTGGCCAATGAA
TTCCTGCGGCGGTTCAACGAAACCAACCGGACGTCGCTGACCATCGCGTCCGATGGCATGGACGTCCTGACAACCTGTTA
TTTTCCGGGCAACGTACGCGAACTGGAAAACTGCATCCGGCGCACCGCGACGCTGGCGCCGGGGCAGACCATCGGCGCGG
CCGACTTTGCCTGTCGCAGCGACGGCTGCCTGTCCTCGATCTTCTGGCGGCCGGCCGCACCCGCCCCGGGTCCGGGCCGC
CATCCGGCGGCATCCGATGTGGCCGGGGACACCGGCGCGGCGACGGAACTGCCCGTTCTTCCGGCGCCCCGGTCTTCGTC
CCAGTCTTCGCCCCAGTCCTCGGCCGCGCCCGACCCGGCGACGTGCCCGTCCTCGGCCGTGTGTTCGGCCGCGCAGGGGG
AATGGAGCCCCCAGCGCGACGAACTGCTCGAAGCCATGGAAACCGCCGGCTGGGTGCAGGCCAAGGCAGCGCGGATTCTG
GGCCTGACACCCCGTCAGATCGGCTACGCCCTGCGCAAGAACGGGATTTCCATCAAGAAATTCTGA

Upstream 100 bases:

>100_bases
ATTTGCTTCGGACCTGAAACGTAGGTTGTCACTGTCCATCCGGGATGTCATGATCCTGACATATTCCGGATACGGCTGGT
GAGGTGGAGGAGGCAAGTAG

Downstream 100 bases:

>100_bases
CGTCCAAAGCCTGTCTGGCTCTCGGATGTCGCGATTGTCGGACATGAGACATTCCTGTCACCCGCGTGGCGGCAGGCCAT
GACAGGACACACGGTTGGCG

Product: Nif-specific regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPTDTVPFRPVSGEGHSTWAERALFGLHEISKILCAPTETVGIIRNVLAVLESFVDLNNAVVALFDAAGNVETIIGTEAD
DAAARRYFDSIPEQAVGQIAVSRQPLLVPDVTGDTRYGFATSQAWTVGAGRIGVIGVPILSRDRLVGVMLLDRPAIPEES
VVGSMDVCLLSMVAGLMGQMVRLHRLVQRDRENLLHDSGLAQPAPPVADGGGYMGIVGDSPALRAVLQKVEIVARSDVTV
LLRGESGTGKELFAQAIHQASPRHRKPFVKLNCAALPESVLESELFGHEKGAFTGAIGQRKGRFELADGGTLFMDEIGEI
SPSFQAKLLRVLQEGEFERVGGTRTLKVDVRVVTATNRNLEEAVSKGRFRADLYYRLSVIPIFLPPLRDRPTDIPMLANE
FLRRFNETNRTSLTIASDGMDVLTTCYFPGNVRELENCIRRTATLAPGQTIGAADFACRSDGCLSSIFWRPAAPAPGPGR
HPAASDVAGDTGAATELPVLPAPRSSSQSSPQSSAAPDPATCPSSAVCSAAQGEWSPQRDELLEAMETAGWVQAKAARIL
GLTPRQIGYALRKNGISIKKF

Sequences:

>Translated_581_residues
MPTDTVPFRPVSGEGHSTWAERALFGLHEISKILCAPTETVGIIRNVLAVLESFVDLNNAVVALFDAAGNVETIIGTEAD
DAAARRYFDSIPEQAVGQIAVSRQPLLVPDVTGDTRYGFATSQAWTVGAGRIGVIGVPILSRDRLVGVMLLDRPAIPEES
VVGSMDVCLLSMVAGLMGQMVRLHRLVQRDRENLLHDSGLAQPAPPVADGGGYMGIVGDSPALRAVLQKVEIVARSDVTV
LLRGESGTGKELFAQAIHQASPRHRKPFVKLNCAALPESVLESELFGHEKGAFTGAIGQRKGRFELADGGTLFMDEIGEI
SPSFQAKLLRVLQEGEFERVGGTRTLKVDVRVVTATNRNLEEAVSKGRFRADLYYRLSVIPIFLPPLRDRPTDIPMLANE
FLRRFNETNRTSLTIASDGMDVLTTCYFPGNVRELENCIRRTATLAPGQTIGAADFACRSDGCLSSIFWRPAAPAPGPGR
HPAASDVAGDTGAATELPVLPAPRSSSQSSPQSSAAPDPATCPSSAVCSAAQGEWSPQRDELLEAMETAGWVQAKAARIL
GLTPRQIGYALRKNGISIKKF
>Mature_580_residues
PTDTVPFRPVSGEGHSTWAERALFGLHEISKILCAPTETVGIIRNVLAVLESFVDLNNAVVALFDAAGNVETIIGTEADD
AAARRYFDSIPEQAVGQIAVSRQPLLVPDVTGDTRYGFATSQAWTVGAGRIGVIGVPILSRDRLVGVMLLDRPAIPEESV
VGSMDVCLLSMVAGLMGQMVRLHRLVQRDRENLLHDSGLAQPAPPVADGGGYMGIVGDSPALRAVLQKVEIVARSDVTVL
LRGESGTGKELFAQAIHQASPRHRKPFVKLNCAALPESVLESELFGHEKGAFTGAIGQRKGRFELADGGTLFMDEIGEIS
PSFQAKLLRVLQEGEFERVGGTRTLKVDVRVVTATNRNLEEAVSKGRFRADLYYRLSVIPIFLPPLRDRPTDIPMLANEF
LRRFNETNRTSLTIASDGMDVLTTCYFPGNVRELENCIRRTATLAPGQTIGAADFACRSDGCLSSIFWRPAAPAPGPGRH
PAASDVAGDTGAATELPVLPAPRSSSQSSPQSSAAPDPATCPSSAVCSAAQGEWSPQRDELLEAMETAGWVQAKAARILG
LTPRQIGYALRKNGISIKKF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=242, Percent_Identity=56.6115702479339, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1790437, Length=259, Percent_Identity=53.2818532818533, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=46.1300309597523, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788550, Length=242, Percent_Identity=54.5454545454545, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=58.6206896551724, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788905, Length=259, Percent_Identity=48.6486486486487, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790299, Length=242, Percent_Identity=50.8264462809917, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1787583, Length=249, Percent_Identity=47.3895582329317, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=48.9795918367347, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789233, Length=244, Percent_Identity=44.672131147541, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081872, Length=365, Percent_Identity=37.2602739726027, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=35.7142857142857, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=38.0566801619433, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62268; Mature: 62137

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDTVPFRPVSGEGHSTWAERALFGLHEISKILCAPTETVGIIRNVLAVLESFVDLNNA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
VVALFDAAGNVETIIGTEADDAAARRYFDSIPEQAVGQIAVSRQPLLVPDVTGDTRYGFA
EEEEEECCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCEE
TSQAWTVGAGRIGVIGVPILSRDRLVGVMLLDRPAIPEESVVGSMDVCLLSMVAGLMGQM
CCCEEEECCCCEEEEECCEECCCCEEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHH
VRLHRLVQRDRENLLHDSGLAQPAPPVADGGGYMGIVGDSPALRAVLQKVEIVARSDVTV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
LLRGESGTGKELFAQAIHQASPRHRKPFVKLNCAALPESVLESELFGHEKGAFTGAIGQR
EEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEECCCCCC
KGRFELADGGTLFMDEIGEISPSFQAKLLRVLQEGEFERVGGTRTLKVDVRVVTATNRNL
CCCEEECCCCEEHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCH
EEAVSKGRFRADLYYRLSVIPIFLPPLRDRPTDIPMLANEFLRRFNETNRTSLTIASDGM
HHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCC
DVLTTCYFPGNVRELENCIRRTATLAPGQTIGAADFACRSDGCLSSIFWRPAAPAPGPGR
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCC
HPAASDVAGDTGAATELPVLPAPRSSSQSSPQSSAAPDPATCPSSAVCSAAQGEWSPQRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHH
ELLEAMETAGWVQAKAARILGLTPRQIGYALRKNGISIKKF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PTDTVPFRPVSGEGHSTWAERALFGLHEISKILCAPTETVGIIRNVLAVLESFVDLNNA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
VVALFDAAGNVETIIGTEADDAAARRYFDSIPEQAVGQIAVSRQPLLVPDVTGDTRYGFA
EEEEEECCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCEE
TSQAWTVGAGRIGVIGVPILSRDRLVGVMLLDRPAIPEESVVGSMDVCLLSMVAGLMGQM
CCCEEEECCCCEEEEECCEECCCCEEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHH
VRLHRLVQRDRENLLHDSGLAQPAPPVADGGGYMGIVGDSPALRAVLQKVEIVARSDVTV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEE
LLRGESGTGKELFAQAIHQASPRHRKPFVKLNCAALPESVLESELFGHEKGAFTGAIGQR
EEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEECCCCCC
KGRFELADGGTLFMDEIGEISPSFQAKLLRVLQEGEFERVGGTRTLKVDVRVVTATNRNL
CCCEEECCCCEEHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEECCCCH
EEAVSKGRFRADLYYRLSVIPIFLPPLRDRPTDIPMLANEFLRRFNETNRTSLTIASDGM
HHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCC
DVLTTCYFPGNVRELENCIRRTATLAPGQTIGAADFACRSDGCLSSIFWRPAAPAPGPGR
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCC
HPAASDVAGDTGAATELPVLPAPRSSSQSSPQSSAAPDPATCPSSAVCSAAQGEWSPQRD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHH
ELLEAMETAGWVQAKAARILGLTPRQIGYALRKNGISIKKF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2664425; 3186446 [H]