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The map label for this gene is lacI [H]

Identifier: 162146147

GI number: 162146147

Start: 327483

End: 328562

Strand: Direct

Name: lacI [H]

Synonym: GDI_0319

Alternate gene names: 162146147

Gene position: 327483-328562 (Clockwise)

Preceding gene: 162146146

Following gene: 162146148

Centisome position: 8.3

GC content: 69.91

Gene sequence:

>1080_bases
ATGGGGCGGTTGGAGGATCCGGTGTCGGCCAAGCCTACCATTCTGGACGTGGCGCGCGTGGCGGGCGTGTCGAAATCGAC
GGTTTCCCTGGTCCTTCAGAAGAACCCGATCGTCAGCGAAGGCGCCCGCAGCCGGGTCCTGGCCGCCATCCGGCAGGTCG
GGTACGTCTATAACCGGAATGCGGCCAACCTGCGCCAGTCCCGTTCGAACGCGATCGGGATCGTCGTCAATGACGTGACC
AACAGCTTCTTCGCCGAGATGGCGGTCGGCATGGACATGATCGTGCAGTCGGCCGGCTTCGTGCAGTTGATGGCCCATAC
GGGCGAGAGCGTGGAACGCCAGGCCGAGGTCCTGTCCGCGATGCTCGAACACGGTGTCTCGGGGATCATCCTGTCGCCCG
CGCGCGGCACGCGCGCCGCCGACCTGAAGGCCCATATGGCGGGGCCGGTCCCGATCGTCCTTGCGGTCCGGGACCTTCCC
GGCCTCAGGGCAACCACCGTCGTCGCCGACAATCGGGCCGGCAGCCACGAGGCCGCGCGCCATCTCATCGGCCTCGGCCA
CTGCCGCATCGCCTGGCTGGGCGGCTTTCCGGATACGTCGGTCTACCAGACGCGCCTGTCCGGCCTGCGCGATGCGATGG
CGGAGGCCGGCCTGCTGTTCGAGGAGGCGTATGCCGTCGAAAGCAACGCCTCGCGGCCGGGTGGGGTCCAGGCCATGGGG
CGCCTGCTGGACATGCCGGGTCATCCGATGGCCTGCATGTGCGTCAATGACGCGGTGGCGTTCGGGGCCTGCGACGGGCT
GCGCGGCGCCGGACTGGAACCGGGGCGGGATTTCGCGATCGTCGGGTTCGACGACGTCATGGGCGCGCGGACGGCCGTCC
CGGCGTTGACGACCGTCGCAACGGACCCCGAGGCGGTCGGCCGGCGCACGGCGCAGATCCTTCTGCACCGCATCAGTTCC
GGCGACGTCCGGCCCGAGACGATCACGACACCGGCACGGCTGGTCGTGCGCGCCAGTTGTGGCGAGCCCTGGTCGGCCGG
ACCGGTCGCGGCGGCAGGCGCCGTCCCCGAAATGCGCTGA

Upstream 100 bases:

>100_bases
TCGCGACCAGCGGGGTGAAGGGCTAGGCAGGACCATGCCCTACTGGCTCCGGCGCATGAACCCATTGTAGCATGCCGGCT
TCCGGCCGGGCGATCCGGCC

Downstream 100 bases:

>100_bases
ACCGACGCGGCTCTGGAACCTTCCAATTAGAAAAAAGGCAAAACAAGAGCGGACCGGAGAGTCGCCCGATGCCTGACGGA
ACGGTGAAGGTTTCGATCGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGRLEDPVSAKPTILDVARVAGVSKSTVSLVLQKNPIVSEGARSRVLAAIRQVGYVYNRNAANLRQSRSNAIGIVVNDVT
NSFFAEMAVGMDMIVQSAGFVQLMAHTGESVERQAEVLSAMLEHGVSGIILSPARGTRAADLKAHMAGPVPIVLAVRDLP
GLRATTVVADNRAGSHEAARHLIGLGHCRIAWLGGFPDTSVYQTRLSGLRDAMAEAGLLFEEAYAVESNASRPGGVQAMG
RLLDMPGHPMACMCVNDAVAFGACDGLRGAGLEPGRDFAIVGFDDVMGARTAVPALTTVATDPEAVGRRTAQILLHRISS
GDVRPETITTPARLVVRASCGEPWSAGPVAAAGAVPEMR

Sequences:

>Translated_359_residues
MGRLEDPVSAKPTILDVARVAGVSKSTVSLVLQKNPIVSEGARSRVLAAIRQVGYVYNRNAANLRQSRSNAIGIVVNDVT
NSFFAEMAVGMDMIVQSAGFVQLMAHTGESVERQAEVLSAMLEHGVSGIILSPARGTRAADLKAHMAGPVPIVLAVRDLP
GLRATTVVADNRAGSHEAARHLIGLGHCRIAWLGGFPDTSVYQTRLSGLRDAMAEAGLLFEEAYAVESNASRPGGVQAMG
RLLDMPGHPMACMCVNDAVAFGACDGLRGAGLEPGRDFAIVGFDDVMGARTAVPALTTVATDPEAVGRRTAQILLHRISS
GDVRPETITTPARLVVRASCGEPWSAGPVAAAGAVPEMR
>Mature_358_residues
GRLEDPVSAKPTILDVARVAGVSKSTVSLVLQKNPIVSEGARSRVLAAIRQVGYVYNRNAANLRQSRSNAIGIVVNDVTN
SFFAEMAVGMDMIVQSAGFVQLMAHTGESVERQAEVLSAMLEHGVSGIILSPARGTRAADLKAHMAGPVPIVLAVRDLPG
LRATTVVADNRAGSHEAARHLIGLGHCRIAWLGGFPDTSVYQTRLSGLRDAMAEAGLLFEEAYAVESNASRPGGVQAMGR
LLDMPGHPMACMCVNDAVAFGACDGLRGAGLEPGRDFAIVGFDDVMGARTAVPALTTVATDPEAVGRRTAQILLHRISSG
DVRPETITTPARLVVRASCGEPWSAGPVAAAGAVPEMR

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=31.0975609756098, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789202, Length=287, Percent_Identity=33.7979094076655, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790369, Length=327, Percent_Identity=28.4403669724771, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=31.2684365781711, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=30.03003003003, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=290, Percent_Identity=31.7241379310345, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789068, Length=339, Percent_Identity=29.2035398230088, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=29.6875, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786540, Length=355, Percent_Identity=27.0422535211268, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787580, Length=335, Percent_Identity=26.865671641791, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790715, Length=321, Percent_Identity=27.1028037383178, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=309, Percent_Identity=27.1844660194175, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=28.0487804878049, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37548; Mature: 37416

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLEDPVSAKPTILDVARVAGVSKSTVSLVLQKNPIVSEGARSRVLAAIRQVGYVYNRN
CCCCCCCCCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
AANLRQSRSNAIGIVVNDVTNSFFAEMAVGMDMIVQSAGFVQLMAHTGESVERQAEVLSA
HHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHH
MLEHGVSGIILSPARGTRAADLKAHMAGPVPIVLAVRDLPGLRATTVVADNRAGSHEAAR
HHHCCCCEEEECCCCCCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHH
HLIGLGHCRIAWLGGFPDTSVYQTRLSGLRDAMAEAGLLFEEAYAVESNASRPGGVQAMG
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
RLLDMPGHPMACMCVNDAVAFGACDGLRGAGLEPGRDFAIVGFDDVMGARTAVPALTTVA
HHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHCC
TDPEAVGRRTAQILLHRISSGDVRPETITTPARLVVRASCGEPWSAGPVAAAGAVPEMR
CCHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHEEEEECCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
GRLEDPVSAKPTILDVARVAGVSKSTVSLVLQKNPIVSEGARSRVLAAIRQVGYVYNRN
CCCCCCCCCCCHHHHHHHHHCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
AANLRQSRSNAIGIVVNDVTNSFFAEMAVGMDMIVQSAGFVQLMAHTGESVERQAEVLSA
HHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHH
MLEHGVSGIILSPARGTRAADLKAHMAGPVPIVLAVRDLPGLRATTVVADNRAGSHEAAR
HHHCCCCEEEECCCCCCCCHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHH
HLIGLGHCRIAWLGGFPDTSVYQTRLSGLRDAMAEAGLLFEEAYAVESNASRPGGVQAMG
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHH
RLLDMPGHPMACMCVNDAVAFGACDGLRGAGLEPGRDFAIVGFDDVMGARTAVPALTTVA
HHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHCC
TDPEAVGRRTAQILLHRISSGDVRPETITTPARLVVRASCGEPWSAGPVAAAGAVPEMR
CCHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHEEEEECCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]