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The map label for this gene is paaF [C]

Identifier: 162146148

GI number: 162146148

Start: 328631

End: 329413

Strand: Direct

Name: paaF [C]

Synonym: GDI_0320

Alternate gene names: 162146148

Gene position: 328631-329413 (Clockwise)

Preceding gene: 162146147

Following gene: 162146149

Centisome position: 8.33

GC content: 72.03

Gene sequence:

>783_bases
ATGCCTGACGGAACGGTGAAGGTTTCGATCGCGGGGCCTGTCGCCACCGTCACCCTGGCGCGTCCGGCCCGGCGCAACGC
GCTCGACCTCGGCATGCTGGACGCGCTGTTCCATGCCTGCGACGAGGTCGATGGTGCCCCGCACGTCCGTGCCGCCATCC
TGACGGGTGAGGGGGACGCGTTCTGCGCGGGGGGCGACATCCATGCCTGGGGCGGAATGGGCCCCGGCGATTTCGGCCAT
AACTGGGTGCGGCATGGCCATCGCGTCTTCGATCGCCTTGCGACGCTGCGGATGCCGCTGATTGCCGCGATCAACGGCCA
CGCCCTGGGCGGCGGCCTGGAGATCGCGGCCACCGCCGATATCCGCATCGCCGAGACGCATGTCCGCGTCGGCCTTCCGG
AGGCCAGTCTGGGCATGGTGCCGGGATGGTCGGGTACCCAGCGCCTTGTCAGGCGCCTGGGGGCGCAGGTCGTGCGCCGG
ATGGCGCTGGGCGGCGAGGTCCTCACGGCCGGCGAGGCGCGCGACCTGGGCATTTTCGACAGCGTCGTGGCCACGGGGAC
CGCCCTGCCGGCCGCGCAACAGGCGGCCGACCGGATCGTCGGGCGTGCGCCGGGCGCGACCGAGGCGATCAAGCTCATGA
TTTCGATCGCCGAGGGCGAGGACCGGGGGGCCGCCGTCGATGCGCTGGCGGCGATCGCCGTTTCGGGCCGCCCCGACATG
ACGGAAGGGGTGACCGCGTTCCGTGAAAAGCGCGCGCCGCGATTCAGGGGCGGCTGGTCGTGA

Upstream 100 bases:

>100_bases
CGGCGGCAGGCGCCGTCCCCGAAATGCGCTGAACCGACGCGGCTCTGGAACCTTCCAATTAGAAAAAAGGCAAAACAAGA
GCGGACCGGAGAGTCGCCCG

Downstream 100 bases:

>100_bases
CCACACGACGGATGGCCAGGCCCTGAGGGGCACGCGACCCGACCCTGCTGCAATCCGCTCCGGGAGAAAGACATGGCATA
TGACTATGTGATTGTGGGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPDGTVKVSIAGPVATVTLARPARRNALDLGMLDALFHACDEVDGAPHVRAAILTGEGDAFCAGGDIHAWGGMGPGDFGH
NWVRHGHRVFDRLATLRMPLIAAINGHALGGGLEIAATADIRIAETHVRVGLPEASLGMVPGWSGTQRLVRRLGAQVVRR
MALGGEVLTAGEARDLGIFDSVVATGTALPAAQQAADRIVGRAPGATEAIKLMISIAEGEDRGAAVDALAAIAVSGRPDM
TEGVTAFREKRAPRFRGGWS

Sequences:

>Translated_260_residues
MPDGTVKVSIAGPVATVTLARPARRNALDLGMLDALFHACDEVDGAPHVRAAILTGEGDAFCAGGDIHAWGGMGPGDFGH
NWVRHGHRVFDRLATLRMPLIAAINGHALGGGLEIAATADIRIAETHVRVGLPEASLGMVPGWSGTQRLVRRLGAQVVRR
MALGGEVLTAGEARDLGIFDSVVATGTALPAAQQAADRIVGRAPGATEAIKLMISIAEGEDRGAAVDALAAIAVSGRPDM
TEGVTAFREKRAPRFRGGWS
>Mature_259_residues
PDGTVKVSIAGPVATVTLARPARRNALDLGMLDALFHACDEVDGAPHVRAAILTGEGDAFCAGGDIHAWGGMGPGDFGHN
WVRHGHRVFDRLATLRMPLIAAINGHALGGGLEIAATADIRIAETHVRVGLPEASLGMVPGWSGTQRLVRRLGAQVVRRM
ALGGEVLTAGEARDLGIFDSVVATGTALPAAQQAADRIVGRAPGATEAIKLMISIAEGEDRGAAVDALAAIAVSGRPDMT
EGVTAFREKRAPRFRGGWS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=31.8548387096774, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=32.520325203252, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=30.2197802197802, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI68989263, Length=153, Percent_Identity=38.562091503268, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=25.1184834123223, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI213417737, Length=211, Percent_Identity=25.1184834123223, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI150378541, Length=266, Percent_Identity=30.0751879699248, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157694520, Length=193, Percent_Identity=24.3523316062176, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=34.1269841269841, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=32.8185328185328, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790281, Length=172, Percent_Identity=33.1395348837209, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788682, Length=148, Percent_Identity=35.8108108108108, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=33.7349397590361, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=27.8195488721804, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=24.6963562753036, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.1428571428571, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=31.1111111111111, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=31.9230769230769, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=31.5217391304348, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=31.1475409836066, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=31.1475409836066, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=195, Percent_Identity=31.7948717948718, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=192, Percent_Identity=28.125, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=187, Percent_Identity=27.807486631016, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=25, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=182, Percent_Identity=25.8241758241758, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=31.8725099601594, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=32.6923076923077, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=32.6923076923077, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650670, Length=267, Percent_Identity=28.0898876404494, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=139, Percent_Identity=29.4964028776978, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571730, Length=139, Percent_Identity=29.4964028776978, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=34.4632768361582, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=34.4632768361582, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357171, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=34.4632768361582, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26939; Mature: 26808

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGTVKVSIAGPVATVTLARPARRNALDLGMLDALFHACDEVDGAPHVRAAILTGEGDA
CCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FCAGGDIHAWGGMGPGDFGHNWVRHGHRVFDRLATLRMPLIAAINGHALGGGLEIAATAD
EEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECC
IRIAETHVRVGLPEASLGMVPGWSGTQRLVRRLGAQVVRRMALGGEVLTAGEARDLGIFD
EEEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHH
SVVATGTALPAAQQAADRIVGRAPGATEAIKLMISIAEGEDRGAAVDALAAIAVSGRPDM
HHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCH
TEGVTAFREKRAPRFRGGWS
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PDGTVKVSIAGPVATVTLARPARRNALDLGMLDALFHACDEVDGAPHVRAAILTGEGDA
CCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FCAGGDIHAWGGMGPGDFGHNWVRHGHRVFDRLATLRMPLIAAINGHALGGGLEIAATAD
EEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECC
IRIAETHVRVGLPEASLGMVPGWSGTQRLVRRLGAQVVRRMALGGEVLTAGEARDLGIFD
EEEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHH
SVVATGTALPAAQQAADRIVGRAPGATEAIKLMISIAEGEDRGAAVDALAAIAVSGRPDM
HHHHCCCCCCHHHHHHHHHHCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCCH
TEGVTAFREKRAPRFRGGWS
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA