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The map label for this gene is glpF [H]

Identifier: 162146098

GI number: 162146098

Start: 279842

End: 280681

Strand: Direct

Name: glpF [H]

Synonym: GDI_0267

Alternate gene names: 162146098

Gene position: 279842-280681 (Clockwise)

Preceding gene: 162146097

Following gene: 162146102

Centisome position: 7.1

GC content: 64.76

Gene sequence:

>840_bases
ATGAGCAGAGAATTTCTCGGCGAGCTGATTTCCGAAGCGCTCGCCGTCCTCATCATCATCGCCTTCGGCGATTCCGTCGC
GGCGATGTACATCCTGTACGATCCCAGTCCCTACCTGAACGCCTACTGGGGCGTGTGCATTTCCTGGGGGCTGGGCGTTA
CCATCGCCATCTATGCGACGGCCTCGGTGTCCGGCACCCACGCCAACCCGGCCGTCACGCTGGCACTGGCGATCTACCGG
CATTTCCCGTGGCGCAAGGTGGTGCCGTACTGCATCGCGCAGATCATCGGCGCCTTCCTCGGGGCTGCCATCGTCTATGC
GCTGTATGTCACCGTCATCGACGATTTCAACACGGTGCACCACCTGACGCGCGCGGCCGGGGGCGCGGCCGGGGTCTTCT
TCACCCATCCGGGCCACGCCATCACGCCCATGCACGCGTTCAGCGACCAGATCATCCTGACCGCCTTCCTGCTGTTCGGA
ATCTTCGCGATTACCGAGCAATATAACGAAATGGCGCCGGGCGCGAATTTCGGTGCCCTGATCATCGGTTTCCTCGTCGC
CCTGATCGGCGCCTCGATGGGCTACCTGGAGGCCTGGGCCATCAACCCCGCCCGTGATTTCGGTCCGCGCCTGTTCGCCT
ATTTCGCCGGATGGGATCAATCGGCCTTCCCCTCGCCGGGGAATTACTGGTGGGTGCCGATCGCCGGGCCGCTGATCGGC
GGCGTCGTGGGGGCCACGGTCTATGAATATCTGGTCCACCCGTTCCTGCCGGCGCACATCCGCGCGCAGAAGGCCCTGCA
CGCCGTGGTCACGACCCCGCCGCCCCGCGCGGCGGAATAG

Upstream 100 bases:

>100_bases
ATTACACGAAATCCTGGAAGAAGGCCGTGCAGCGGTCCTTCGACTGGGTGGATTGACGATCGCGGGCGCCTGCAACGAGA
CCATGTGGGGGGAAGAACCC

Downstream 100 bases:

>100_bases
GGACGACGGAGCAGGGGGAACAGGGACCGGCCGCGTGATTCCCCGGGGAATCACGCGGCCTTTCCCGTATCATACCCGGC
CGTAGGCGAAGCTGTTGCGC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSREFLGELISEALAVLIIIAFGDSVAAMYILYDPSPYLNAYWGVCISWGLGVTIAIYATASVSGTHANPAVTLALAIYR
HFPWRKVVPYCIAQIIGAFLGAAIVYALYVTVIDDFNTVHHLTRAAGGAAGVFFTHPGHAITPMHAFSDQIILTAFLLFG
IFAITEQYNEMAPGANFGALIIGFLVALIGASMGYLEAWAINPARDFGPRLFAYFAGWDQSAFPSPGNYWWVPIAGPLIG
GVVGATVYEYLVHPFLPAHIRAQKALHAVVTTPPPRAAE

Sequences:

>Translated_279_residues
MSREFLGELISEALAVLIIIAFGDSVAAMYILYDPSPYLNAYWGVCISWGLGVTIAIYATASVSGTHANPAVTLALAIYR
HFPWRKVVPYCIAQIIGAFLGAAIVYALYVTVIDDFNTVHHLTRAAGGAAGVFFTHPGHAITPMHAFSDQIILTAFLLFG
IFAITEQYNEMAPGANFGALIIGFLVALIGASMGYLEAWAINPARDFGPRLFAYFAGWDQSAFPSPGNYWWVPIAGPLIG
GVVGATVYEYLVHPFLPAHIRAQKALHAVVTTPPPRAAE
>Mature_278_residues
SREFLGELISEALAVLIIIAFGDSVAAMYILYDPSPYLNAYWGVCISWGLGVTIAIYATASVSGTHANPAVTLALAIYRH
FPWRKVVPYCIAQIIGAFLGAAIVYALYVTVIDDFNTVHHLTRAAGGAAGVFFTHPGHAITPMHAFSDQIILTAFLLFGI
FAITEQYNEMAPGANFGALIIGFLVALIGASMGYLEAWAINPARDFGPRLFAYFAGWDQSAFPSPGNYWWVPIAGPLIGG
VVGATVYEYLVHPFLPAHIRAQKALHAVVTTPPPRAAE

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=37.7510040160643, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI4502187, Length=258, Percent_Identity=34.8837209302326, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI157266307, Length=250, Percent_Identity=35.6, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI22538420, Length=253, Percent_Identity=32.4110671936759, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310133356, Length=209, Percent_Identity=35.8851674641148, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI310114181, Length=209, Percent_Identity=35.8851674641148, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4755125, Length=231, Percent_Identity=30.7359307359307, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4502181, Length=231, Percent_Identity=30.7359307359307, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4502179, Length=255, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI6912506, Length=216, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502183, Length=255, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=278, Percent_Identity=40.6474820143885, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71992966, Length=254, Percent_Identity=34.251968503937, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17544068, Length=266, Percent_Identity=36.0902255639098, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994009, Length=264, Percent_Identity=30.6818181818182, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=263, Percent_Identity=31.5589353612167, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=263, Percent_Identity=31.5589353612167, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71992961, Length=254, Percent_Identity=31.8897637795276, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=273, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17533613, Length=273, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321054, Length=220, Percent_Identity=39.5454545454545, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322985, Length=212, Percent_Identity=32.5471698113208, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24762342, Length=251, Percent_Identity=27.0916334661355, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550503, Length=210, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30019; Mature: 29888

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREFLGELISEALAVLIIIAFGDSVAAMYILYDPSPYLNAYWGVCISWGLGVTIAIYAT
CCHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEE
ASVSGTHANPAVTLALAIYRHFPWRKVVPYCIAQIIGAFLGAAIVYALYVTVIDDFNTVH
EECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLTRAAGGAAGVFFTHPGHAITPMHAFSDQIILTAFLLFGIFAITEQYNEMAPGANFGAL
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIGFLVALIGASMGYLEAWAINPARDFGPRLFAYFAGWDQSAFPSPGNYWWVPIAGPLIG
HHHHHHHHHCCCCCHHHEEECCCHHHHCHHHHHHEECCCCCCCCCCCCEEEEEECHHHHH
GVVGATVYEYLVHPFLPAHIRAQKALHAVVTTPPPRAAE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure 
SREFLGELISEALAVLIIIAFGDSVAAMYILYDPSPYLNAYWGVCISWGLGVTIAIYAT
CHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEE
ASVSGTHANPAVTLALAIYRHFPWRKVVPYCIAQIIGAFLGAAIVYALYVTVIDDFNTVH
EECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLTRAAGGAAGVFFTHPGHAITPMHAFSDQIILTAFLLFGIFAITEQYNEMAPGANFGAL
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIGFLVALIGASMGYLEAWAINPARDFGPRLFAYFAGWDQSAFPSPGNYWWVPIAGPLIG
HHHHHHHHHCCCCCHHHEEECCCHHHHCHHHHHHEECCCCCCCCCCCCEEEEEECHHHHH
GVVGATVYEYLVHPFLPAHIRAQKALHAVVTTPPPRAAE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]