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The map label for this gene is glpK [H]

Identifier: 162146097

GI number: 162146097

Start: 278298

End: 279797

Strand: Direct

Name: glpK [H]

Synonym: GDI_0266

Alternate gene names: 162146097

Gene position: 278298-279797 (Clockwise)

Preceding gene: 162146096

Following gene: 162146098

Centisome position: 7.06

GC content: 65.53

Gene sequence:

>1500_bases
ATGACCAGCTATGTCGGCGCGATCGACCAGGGAACGACCAGTTCGCGCTTCATCATCTTCGACCGCCAAGGCCGGATCGC
CTCGGTCGCGCAGAAGGAACATCGACAGATCTATCCCCGCCCGGGCTGGGTGGAACATGACCCTGTCGAAATCATGAACA
ACACCAACGAAATGATCGGGGCGGCGCTGGCCCGCGCCAACCTGGCGGCGGAGGACCTGGCCGCCGTCGGCATCACCAAC
CAGCGGGAAACGACCGTCCTGTGGGACCGGGAAACCGGCAGGCCGCTGCACAACGCCCTGGTGTGGCAGGATACGCGGGT
CGATCAACTGGTCACGACATTCGCCGCCGACGGGGGGCAGGACCGTTTCCGCCAGGTCACCGGCCTGCCGCTGGCCAGTT
ACTTCGCGGGGCTGAAGCTCCGCTGGCTGCTGGACAACGTGGATGGCGCGCGCGCGAAGGCGGAAGCGGGGCAGGCCCTG
TTCGGCACCATCGACAGCTGGGTCATCTGGAACCTGACCGGCGGGCCGAACGGCGGCATCCATGTGACGGACGTCACCAA
CGCCTCGCGCACCCAGTTGATGAACCTGGAAACCTGCGACTGGGATCGCGGCATGCTGGACGTCTTCGCCATTCCCGCCG
CATGCCTGCCCAAAATCGTGCCCTCATCCTTCGTGTATGGTGAAATCGCCACGCCGACGCTGAAGGGTACGAAGATCGCC
GGCATCCTGGGCGACCAGCAGGCCGCCCTGGTCGGCCAGACCTGCTTTTCACCGGGCGAGGCCAAGAACACCTACGGCAC
GGGCTCGTTCCTGCTGATGAACACGGGCGTCAAGCCGGTGCAGTCCAAGGCGGGCCTGCTGACGACGCTGGCCTACCAGT
TCGGCGAGGAACGCCCCTGCTACGCGCTGGAGGGCGCCATCGCCATCACCGGGGCGCTGGTGCAATGGCTGCGCGACAAT
CTGAAACTGTTCGATATCGCGACCCAGATCGAACCGCTGGCCCGCAGCGTGGAGGATAACGGCGGTATCTATATCGTGCC
GGCCTTCTCCGGCCTGTACGCGCCGTACTGGAAGGAAAACGCCCGTGGCGTGATCGCCGGGCTGACGCGCTACGTCACCC
GGGCGCATTTCGCCCGCGCGGCGCTGGAATCCACCGCCTATCAGGTGCGCGACGTGGTGGAGGCGATGGAAAGCGACAGC
GGCATCAGGCTGACCAGCCTGAAGACCGACGGCGGCATGGTGGCCAACGAACTGCTGATGCAGTTCCAGGCCGACATCCT
GAACGTTCCCGTCGTCCGACCCAAGGTCGTCGAAACCACGGCCCTCGGCGCCGCCTACGCGGCGGGGCTGGCCGTGGGGT
ACTGGAGCAACACGGACGACCTGCGCGCCAACTGGGAAATCGACAAGACCTGGACACCGTCCATGCCGGAAGACCGCCGC
GCCCATTACACGAAATCCTGGAAGAAGGCCGTGCAGCGGTCCTTCGACTGGGTGGATTGA

Upstream 100 bases:

>100_bases
TCGCATGGTTTTTCGCAAACATCACGTGATATAAGGAAAATTTCAGAAAACCGCCAAGCGGTATGCCGCAAGAAGAAAAA
CCGTAAAGGGAGCAGGCACC

Downstream 100 bases:

>100_bases
CGATCGCGGGCGCCTGCAACGAGACCATGTGGGGGGAAGAACCCATGAGCAGAGAATTTCTCGGCGAGCTGATTTCCGAA
GCGCTCGCCGTCCTCATCAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTSYVGAIDQGTTSSRFIIFDRQGRIASVAQKEHRQIYPRPGWVEHDPVEIMNNTNEMIGAALARANLAAEDLAAVGITN
QRETTVLWDRETGRPLHNALVWQDTRVDQLVTTFAADGGQDRFRQVTGLPLASYFAGLKLRWLLDNVDGARAKAEAGQAL
FGTIDSWVIWNLTGGPNGGIHVTDVTNASRTQLMNLETCDWDRGMLDVFAIPAACLPKIVPSSFVYGEIATPTLKGTKIA
GILGDQQAALVGQTCFSPGEAKNTYGTGSFLLMNTGVKPVQSKAGLLTTLAYQFGEERPCYALEGAIAITGALVQWLRDN
LKLFDIATQIEPLARSVEDNGGIYIVPAFSGLYAPYWKENARGVIAGLTRYVTRAHFARAALESTAYQVRDVVEAMESDS
GIRLTSLKTDGGMVANELLMQFQADILNVPVVRPKVVETTALGAAYAAGLAVGYWSNTDDLRANWEIDKTWTPSMPEDRR
AHYTKSWKKAVQRSFDWVD

Sequences:

>Translated_499_residues
MTSYVGAIDQGTTSSRFIIFDRQGRIASVAQKEHRQIYPRPGWVEHDPVEIMNNTNEMIGAALARANLAAEDLAAVGITN
QRETTVLWDRETGRPLHNALVWQDTRVDQLVTTFAADGGQDRFRQVTGLPLASYFAGLKLRWLLDNVDGARAKAEAGQAL
FGTIDSWVIWNLTGGPNGGIHVTDVTNASRTQLMNLETCDWDRGMLDVFAIPAACLPKIVPSSFVYGEIATPTLKGTKIA
GILGDQQAALVGQTCFSPGEAKNTYGTGSFLLMNTGVKPVQSKAGLLTTLAYQFGEERPCYALEGAIAITGALVQWLRDN
LKLFDIATQIEPLARSVEDNGGIYIVPAFSGLYAPYWKENARGVIAGLTRYVTRAHFARAALESTAYQVRDVVEAMESDS
GIRLTSLKTDGGMVANELLMQFQADILNVPVVRPKVVETTALGAAYAAGLAVGYWSNTDDLRANWEIDKTWTPSMPEDRR
AHYTKSWKKAVQRSFDWVD
>Mature_498_residues
TSYVGAIDQGTTSSRFIIFDRQGRIASVAQKEHRQIYPRPGWVEHDPVEIMNNTNEMIGAALARANLAAEDLAAVGITNQ
RETTVLWDRETGRPLHNALVWQDTRVDQLVTTFAADGGQDRFRQVTGLPLASYFAGLKLRWLLDNVDGARAKAEAGQALF
GTIDSWVIWNLTGGPNGGIHVTDVTNASRTQLMNLETCDWDRGMLDVFAIPAACLPKIVPSSFVYGEIATPTLKGTKIAG
ILGDQQAALVGQTCFSPGEAKNTYGTGSFLLMNTGVKPVQSKAGLLTTLAYQFGEERPCYALEGAIAITGALVQWLRDNL
KLFDIATQIEPLARSVEDNGGIYIVPAFSGLYAPYWKENARGVIAGLTRYVTRAHFARAALESTAYQVRDVVEAMESDSG
IRLTSLKTDGGMVANELLMQFQADILNVPVVRPKVVETTALGAAYAAGLAVGYWSNTDDLRANWEIDKTWTPSMPEDRRA
HYTKSWKKAVQRSFDWVD

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=49.0079365079365, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=49.0079365079365, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=48.5089463220676, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=48.4313725490196, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=30.7992202729045, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=52.3138832997988, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790005, Length=472, Percent_Identity=23.5169491525424, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787789, Length=526, Percent_Identity=26.615969581749, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789987, Length=437, Percent_Identity=23.3409610983982, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789168, Length=472, Percent_Identity=23.3050847457627, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=43.9363817097416, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=24.8565965583174, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71986147, Length=267, Percent_Identity=31.0861423220974, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=552, Percent_Identity=38.4057971014493, Blast_Score=335, Evalue=8e-93,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=48.4189723320158, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24655555, Length=509, Percent_Identity=43.4184675834971, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655560, Length=509, Percent_Identity=43.4184675834971, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655570, Length=509, Percent_Identity=43.4184675834971, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655574, Length=509, Percent_Identity=43.4184675834971, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655565, Length=509, Percent_Identity=43.4184675834971, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24654573, Length=457, Percent_Identity=48.1400437636761, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=35.7815442561205, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=36.6598778004073, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24656367, Length=517, Percent_Identity=29.2069632495164, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=28.6527514231499, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=30.8571428571429, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45552909, Length=330, Percent_Identity=28.4848484848485, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54630; Mature: 54499

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYVGAIDQGTTSSRFIIFDRQGRIASVAQKEHRQIYPRPGWVEHDPVEIMNNTNEMIG
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHH
AALARANLAAEDLAAVGITNQRETTVLWDRETGRPLHNALVWQDTRVDQLVTTFAADGGQ
HHHHHHHCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHEECCCHHHHHHHHHHCCCCH
DRFRQVTGLPLASYFAGLKLRWLLDNVDGARAKAEAGQALFGTIDSWVIWNLTGGPNGGI
HHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHHHHCCCHHEECCCCEEEEEECCCCCCCE
HVTDVTNASRTQLMNLETCDWDRGMLDVFAIPAACLPKIVPSSFVYGEIATPTLKGTKIA
EEEECCCCCHHHEECCCCCCCCCCCHHHHHCCHHHHHHHCCCCEEECCCCCCCCCCCEEE
GILGDQQAALVGQTCFSPGEAKNTYGTGSFLLMNTGVKPVQSKAGLLTTLAYQFGEERPC
EEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
YALEGAIAITGALVQWLRDNLKLFDIATQIEPLARSVEDNGGIYIVPAFSGLYAPYWKEN
EEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHCC
ARGVIAGLTRYVTRAHFARAALESTAYQVRDVVEAMESDSGIRLTSLKTDGGMVANELLM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
QFQADILNVPVVRPKVVETTALGAAYAAGLAVGYWSNTDDLRANWEIDKTWTPSMPEDRR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEECEEECCCCCCCCCCHHH
AHYTKSWKKAVQRSFDWVD
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSYVGAIDQGTTSSRFIIFDRQGRIASVAQKEHRQIYPRPGWVEHDPVEIMNNTNEMIG
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHH
AALARANLAAEDLAAVGITNQRETTVLWDRETGRPLHNALVWQDTRVDQLVTTFAADGGQ
HHHHHHHCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHEECCCHHHHHHHHHHCCCCH
DRFRQVTGLPLASYFAGLKLRWLLDNVDGARAKAEAGQALFGTIDSWVIWNLTGGPNGGI
HHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHHHHCCCHHEECCCCEEEEEECCCCCCCE
HVTDVTNASRTQLMNLETCDWDRGMLDVFAIPAACLPKIVPSSFVYGEIATPTLKGTKIA
EEEECCCCCHHHEECCCCCCCCCCCHHHHHCCHHHHHHHCCCCEEECCCCCCCCCCCEEE
GILGDQQAALVGQTCFSPGEAKNTYGTGSFLLMNTGVKPVQSKAGLLTTLAYQFGEERPC
EEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
YALEGAIAITGALVQWLRDNLKLFDIATQIEPLARSVEDNGGIYIVPAFSGLYAPYWKEN
EEECCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHCC
ARGVIAGLTRYVTRAHFARAALESTAYQVRDVVEAMESDSGIRLTSLKTDGGMVANELLM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
QFQADILNVPVVRPKVVETTALGAAYAAGLAVGYWSNTDDLRANWEIDKTWTPSMPEDRR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEECEEECCCCCCCCCCHHH
AHYTKSWKKAVQRSFDWVD
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA