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The map label for this gene is dhaK [H]

Identifier: 162146094

GI number: 162146094

Start: 274247

End: 275248

Strand: Direct

Name: dhaK [H]

Synonym: GDI_0263

Alternate gene names: 162146094

Gene position: 274247-275248 (Clockwise)

Preceding gene: 162146093

Following gene: 162146095

Centisome position: 6.95

GC content: 69.86

Gene sequence:

>1002_bases
ATGAAGAAGCTCATCAACGCCCCCGACCGGGTCGTCACCGAAGCCCTGGAGGGCATGGCGGCGGCCCACCCCGACCTGCT
GACCGTCCATCTCGACCCCGCCTACATCATGCGCGCCGACGCGCCCGTGAAGGGCAAGGCCGCGCTGGTCTCGGGCGGCG
GATCGGGGCACGAACCGATGCATGGCGGCTATGTGGGGCGCGGCATGCTGGACGCCGCCTGTCCGGGCGCGGTCTTCACC
AGCCCGACCCCCGACCAGATCCTGGCGGCGACCCGCGCGGTGACGGGCGAGCAGGGGGCGCTGCACATCGTCAAGAACTA
CACGGGCGACGTCATGAATTTCGACATGGCGGCCGACCTGGCGCGGGAGGAAGGATTGCGGCTGGAGGCCGTGATCATCG
ACGACGACGTGGCGGTCAAGGACAGCCTCTACACGGCGGGGCGGCGCGGCGTCGGCGGCACGATCCTGGCGGAGAAGATC
TGCGGCGGCGCGGCCGAGGCCGGGGCGGACCTCGGGTCGCTGGCCCGCCTGTGCCGGGACGTGAACGCGTCCGTCCGCTC
GATGGGCGTGGCGCTGACGTCCTGCACCGTGCCGCATGTCGGAAAACCCAGTTTCGAACTGGGGGAAGACGAAATGGAAT
TCGGCATCGGCATCCACGGCGAACCCGGACGGAAGCGCGTCGGGATGGAAAGCGCCGACCGCATCGCGGACATGCTGATG
CAGCCGATCCTGGAGGACCTGCCGTTCCGCCGGGGCGACCCGGTCCTGCTGTTCGTCAACGGCCTGGGCGGCACGCCGCT
GATCGAACTGTATATCGTCTATCGCGCGGCCCACCGGATCGCCCAGGCACATGGGCTGACCGTCGCCCGCTCGCTGGTCG
GGTCCTACATCACCAGCCTGGAAATGGCCGGCACGTCGATCACGCTGCTGCGCCTGACGGACGAGATGACCCGCTACTGG
GACGCCCCGTGCGTCACGCCCGCCCTGCGCTGGGGGGCGTGA

Upstream 100 bases:

>100_bases
TATATCGCGCATCGCGGCAAGCCCGTGGAATCGCAGGTGGCGGATGTAGCCTAGGACGTATCGGATTCGCCCCGCCCGAA
CAGGTCCCTGGAGACCAGGC

Downstream 100 bases:

>100_bases
GCGCATGCCGGATGCCCGCACGGCGGATGTCGCCTTGTTGTGCGTCTGGCTGCGCCGGGCGGCACAGGTCATCGACGACC
GGCAGGACAGCCTGTCGGTG

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKLINAPDRVVTEALEGMAAAHPDLLTVHLDPAYIMRADAPVKGKAALVSGGGSGHEPMHGGYVGRGMLDAACPGAVFT
SPTPDQILAATRAVTGEQGALHIVKNYTGDVMNFDMAADLAREEGLRLEAVIIDDDVAVKDSLYTAGRRGVGGTILAEKI
CGGAAEAGADLGSLARLCRDVNASVRSMGVALTSCTVPHVGKPSFELGEDEMEFGIGIHGEPGRKRVGMESADRIADMLM
QPILEDLPFRRGDPVLLFVNGLGGTPLIELYIVYRAAHRIAQAHGLTVARSLVGSYITSLEMAGTSITLLRLTDEMTRYW
DAPCVTPALRWGA

Sequences:

>Translated_333_residues
MKKLINAPDRVVTEALEGMAAAHPDLLTVHLDPAYIMRADAPVKGKAALVSGGGSGHEPMHGGYVGRGMLDAACPGAVFT
SPTPDQILAATRAVTGEQGALHIVKNYTGDVMNFDMAADLAREEGLRLEAVIIDDDVAVKDSLYTAGRRGVGGTILAEKI
CGGAAEAGADLGSLARLCRDVNASVRSMGVALTSCTVPHVGKPSFELGEDEMEFGIGIHGEPGRKRVGMESADRIADMLM
QPILEDLPFRRGDPVLLFVNGLGGTPLIELYIVYRAAHRIAQAHGLTVARSLVGSYITSLEMAGTSITLLRLTDEMTRYW
DAPCVTPALRWGA
>Mature_333_residues
MKKLINAPDRVVTEALEGMAAAHPDLLTVHLDPAYIMRADAPVKGKAALVSGGGSGHEPMHGGYVGRGMLDAACPGAVFT
SPTPDQILAATRAVTGEQGALHIVKNYTGDVMNFDMAADLAREEGLRLEAVIIDDDVAVKDSLYTAGRRGVGGTILAEKI
CGGAAEAGADLGSLARLCRDVNASVRSMGVALTSCTVPHVGKPSFELGEDEMEFGIGIHGEPGRKRVGMESADRIADMLM
QPILEDLPFRRGDPVLLFVNGLGGTPLIELYIVYRAAHRIAQAHGLTVARSLVGSYITSLEMAGTSITLLRLTDEMTRYW
DAPCVTPALRWGA

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=338, Percent_Identity=43.1952662721894, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=52.247191011236, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17565018, Length=326, Percent_Identity=36.5030674846626, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321055, Length=345, Percent_Identity=37.6811594202899, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323570, Length=317, Percent_Identity=37.2239747634069, Blast_Score=183, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35174; Mature: 35174

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINAPDRVVTEALEGMAAAHPDLLTVHLDPAYIMRADAPVKGKAALVSGGGSGHEPM
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCC
HGGYVGRGMLDAACPGAVFTSPTPDQILAATRAVTGEQGALHIVKNYTGDVMNFDMAADL
CCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEHHHHHHH
AREEGLRLEAVIIDDDVAVKDSLYTAGRRGVGGTILAEKICGGAAEAGADLGSLARLCRD
HHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHH
VNASVRSMGVALTSCTVPHVGKPSFELGEDEMEFGIGIHGEPGRKRVGMESADRIADMLM
HCHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
QPILEDLPFRRGDPVLLFVNGLGGTPLIELYIVYRAAHRIAQAHGLTVARSLVGSYITSL
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAGTSITLLRLTDEMTRYWDAPCVTPALRWGA
HHCCCEEEEEEEHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MKKLINAPDRVVTEALEGMAAAHPDLLTVHLDPAYIMRADAPVKGKAALVSGGGSGHEPM
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCC
HGGYVGRGMLDAACPGAVFTSPTPDQILAATRAVTGEQGALHIVKNYTGDVMNFDMAADL
CCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCEEHHHHHHH
AREEGLRLEAVIIDDDVAVKDSLYTAGRRGVGGTILAEKICGGAAEAGADLGSLARLCRD
HHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHH
VNASVRSMGVALTSCTVPHVGKPSFELGEDEMEFGIGIHGEPGRKRVGMESADRIADMLM
HCHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
QPILEDLPFRRGDPVLLFVNGLGGTPLIELYIVYRAAHRIAQAHGLTVARSLVGSYITSL
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAGTSITLLRLTDEMTRYWDAPCVTPALRWGA
HHCCCEEEEEEEHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]