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The map label for this gene is qor [C]

Identifier: 162146091

GI number: 162146091

Start: 270521

End: 271567

Strand: Direct

Name: qor [C]

Synonym: GDI_0260

Alternate gene names: 162146091

Gene position: 270521-271567 (Clockwise)

Preceding gene: 162146090

Following gene: 162146092

Centisome position: 6.86

GC content: 69.72

Gene sequence:

>1047_bases
ATGTCCCGTCCTATCCCAATGTCCCTGTGCGCCATCAACAGGGGAACGAGAATGCCGGACGCATTGCGGAGCCGTTATCG
GGCCTGGAGCTGGGACGCGCCCGGCGAACCGGATGAACTTCAACTGGTGGAAAAAGCGACGGTCCAGCCCGCATCGGGCG
AAATCGTCGTGGAAAATACCCTGGCCGCGTTCAACCCGGTGGACTGGAAGATCATCCAGAACGGCCATGCCGCCTGGAAA
TCCGGCCGCGTTCCGGGGGTCGACGGGGTCGGCCGGATCGCCGCCGTGGGCCCCGATGTCCGCCTGCCGGTCGGCCAGCG
GGTCGCGTATCACCAGAGCCTGGCGCGGGACGGCAGTTTCTCGGACTACACCACCCTGCGGGCCGAGAGCGTCCTGCCGG
TTCCCGACGGCGTCAGTGACGAGGTCGCGGCCGGCATTCCCTGCCCCGGCCTGACGGCGTGGCAGGCGCTGGCCAAGGTC
CCGTCCGTTCCCGATCGCGATGTGCTGGTGACGGGGGCAGGGGGCGCGGTCGCGCTGATCCTCGCGCAACTGGCGGTGCG
GCGGGGGTGGCGGGTCTGGGTCACGGCGTCGCCCCGCCATCACGCGCGCCTGCTGGGCCTGGGGGTCTGCGGGGCGTTCG
ATTATCAGGACGATTCCTGGCAGGGCGCGTTGCAACAGGCGCTGGGGCCGCGCCGCCTGTTCGCCGTGTTCGACACCACA
AGCGGGGGCTATGCGAGGACGCTGGCGCCGATGCTCGGCTTCAACGGGCATCTGGTCTGCATCCAGGACCGGCTGGACGC
GCCGCCCCTGCCGCCGTTCGCCACCGCGCTCTCGTTGCACGAGGTGGCGCTGAACGCGATGCACGACCACGCGTCGCCAC
AGGACTGGCGCGACTGGCGCATGGCCGGCGCCGGCCTGATGCACGACGTGCAGGCCGGCGCGCTGGAAACGCAGCCCGTC
CGCGTGACGGCGTTCACGGCCCTGCCCGAAAGCCTGTCCGCCTTCAAGGCCGGCCGGACAACGGGAAAAGTGCTGGTGAA
GATATAG

Upstream 100 bases:

>100_bases
GGCTGATTCCCTGATAACGGCTCCGGCGATAACGGTCCCGGCGGGCGGGCGCGTCATGATGCCAGACGGACTCTGATATC
AGGATTCTCCAGCTTCAATA

Downstream 100 bases:

>100_bases
GACATTCGAACGCATGGCATCGCCGGACGATATGGGTGATGCCATCGCCCCTCCCGCGTCCCTCGTGGCACGCCGGATGA
CCGGAGACACGATCCGTACC

Product: NADPH quinone oxidoreductase protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSRPIPMSLCAINRGTRMPDALRSRYRAWSWDAPGEPDELQLVEKATVQPASGEIVVENTLAAFNPVDWKIIQNGHAAWK
SGRVPGVDGVGRIAAVGPDVRLPVGQRVAYHQSLARDGSFSDYTTLRAESVLPVPDGVSDEVAAGIPCPGLTAWQALAKV
PSVPDRDVLVTGAGGAVALILAQLAVRRGWRVWVTASPRHHARLLGLGVCGAFDYQDDSWQGALQQALGPRRLFAVFDTT
SGGYARTLAPMLGFNGHLVCIQDRLDAPPLPPFATALSLHEVALNAMHDHASPQDWRDWRMAGAGLMHDVQAGALETQPV
RVTAFTALPESLSAFKAGRTTGKVLVKI

Sequences:

>Translated_348_residues
MSRPIPMSLCAINRGTRMPDALRSRYRAWSWDAPGEPDELQLVEKATVQPASGEIVVENTLAAFNPVDWKIIQNGHAAWK
SGRVPGVDGVGRIAAVGPDVRLPVGQRVAYHQSLARDGSFSDYTTLRAESVLPVPDGVSDEVAAGIPCPGLTAWQALAKV
PSVPDRDVLVTGAGGAVALILAQLAVRRGWRVWVTASPRHHARLLGLGVCGAFDYQDDSWQGALQQALGPRRLFAVFDTT
SGGYARTLAPMLGFNGHLVCIQDRLDAPPLPPFATALSLHEVALNAMHDHASPQDWRDWRMAGAGLMHDVQAGALETQPV
RVTAFTALPESLSAFKAGRTTGKVLVKI
>Mature_347_residues
SRPIPMSLCAINRGTRMPDALRSRYRAWSWDAPGEPDELQLVEKATVQPASGEIVVENTLAAFNPVDWKIIQNGHAAWKS
GRVPGVDGVGRIAAVGPDVRLPVGQRVAYHQSLARDGSFSDYTTLRAESVLPVPDGVSDEVAAGIPCPGLTAWQALAKVP
SVPDRDVLVTGAGGAVALILAQLAVRRGWRVWVTASPRHHARLLGLGVCGAFDYQDDSWQGALQQALGPRRLFAVFDTTS
GGYARTLAPMLGFNGHLVCIQDRLDAPPLPPFATALSLHEVALNAMHDHASPQDWRDWRMAGAGLMHDVQAGALETQPVR
VTAFTALPESLSAFKAGRTTGKVLVKI

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790485, Length=216, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=258, Percent_Identity=29.8449612403101, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 37289; Mature: 37158

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPIPMSLCAINRGTRMPDALRSRYRAWSWDAPGEPDELQLVEKATVQPASGEIVVENT
CCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECC
LAAFNPVDWKIIQNGHAAWKSGRVPGVDGVGRIAAVGPDVRLPVGQRVAYHQSLARDGSF
HHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
SDYTTLRAESVLPVPDGVSDEVAAGIPCPGLTAWQALAKVPSVPDRDVLVTGAGGAVALI
CCCEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHH
LAQLAVRRGWRVWVTASPRHHARLLGLGVCGAFDYQDDSWQGALQQALGPRRLFAVFDTT
HHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEEECC
SGGYARTLAPMLGFNGHLVCIQDRLDAPPLPPFATALSLHEVALNAMHDHASPQDWRDWR
CCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
MAGAGLMHDVQAGALETQPVRVTAFTALPESLSAFKAGRTTGKVLVKI
HHCCCHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
SRPIPMSLCAINRGTRMPDALRSRYRAWSWDAPGEPDELQLVEKATVQPASGEIVVENT
CCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECC
LAAFNPVDWKIIQNGHAAWKSGRVPGVDGVGRIAAVGPDVRLPVGQRVAYHQSLARDGSF
HHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
SDYTTLRAESVLPVPDGVSDEVAAGIPCPGLTAWQALAKVPSVPDRDVLVTGAGGAVALI
CCCEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHH
LAQLAVRRGWRVWVTASPRHHARLLGLGVCGAFDYQDDSWQGALQQALGPRRLFAVFDTT
HHHHHHHCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEEECC
SGGYARTLAPMLGFNGHLVCIQDRLDAPPLPPFATALSLHEVALNAMHDHASPQDWRDWR
CCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
MAGAGLMHDVQAGALETQPVRVTAFTALPESLSAFKAGRTTGKVLVKI
HHCCCHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]