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The map label for this gene is yafC [H]

Identifier: 162146089

GI number: 162146089

Start: 268284

End: 269153

Strand: Reverse

Name: yafC [H]

Synonym: GDI_0258

Alternate gene names: 162146089

Gene position: 269153-268284 (Counterclockwise)

Preceding gene: 162146099

Following gene: 162146088

Centisome position: 6.82

GC content: 68.74

Gene sequence:

>870_bases
ATGACGCCGTCGGCCGTCAGCAAGCTGGTGTCGCGGCTGGAGCGGCGGCTGGGGACCCGCCTGTTCAACCGGTCCACGCG
GCAACTGCAACTGACGCCGGAGGGCGCCGCGTTCTACGACCGCAGCGTACGTATCCTGGCCGACCTGGCGGAGGCCGAGC
AATGCGCGTCGGCCAGCGCGCTGCCCCACGGGCGGGTCCGCATCAACGTCAACGTGCCGATGGGGCGCCGTTTCCTGCTG
CCGCTGGTTCCGGCGCTGTCGGCCCGGTATCCGGACCTGGTCCTGGATATCGTGCTGACGGATACGGTGGTGGACCTGAT
GGAGGACCGCACGGACATCGCCATCCGCCACGGCCCCCTGAAAAGCTCGCGCCTGATGGCGCGCAAGCTGGGCCGGATGC
GGATGAGGCTGGTCGCCGCCCCGGCCTACCTGGCGCGGTACGGGATGCCCGGAACGCCGGCGGACCTGCGGCGGCACAGG
CGGATCGGGTCCAGCTACTGGCGCATGCATGACGGCTGGCCGCAACTGGACGATGGCGGCCCCGTGCCCGTCCTGCCGCC
CGGCATCGTCCAGGCCAGCGACGGCGATGCGGTCCTGCAACTGGCGATTGCGGGAATGGGCCTGGCCTATCTGGCCGCGT
TCCAGGTCGACGAACATATCGCGGCCGGCAAGCTTGTTCCGGTGCTGGAGGCGTTCCACAGTCCGGCCCTTGTGGATACG
CATGCGGTCTATCTGGGCCATCATGGGAAATTGCCCGCGCGGATCAGGGCGGTGCTGGACTTTTTGCAGGAAAACATGCG
GATCGGGAACGGCGAAGGAATGGTCGCCGAGCATGGCCGCGTGGCGGCATCATGGATGGCCGACGGGTAG

Upstream 100 bases:

>100_bases
CATCATGGGACGATTGGAGATCAACCGTGCGGGCGAGATGGACGTCTTCGTCCGCGTCGTGGACGCCGGGGGCTTCACGC
CGGCCGCGCGGCTGTCGCGC

Downstream 100 bases:

>100_bases
CATCCATCCCCTGTATCATGCCAATGTCATCATTGCGGTATCGATCGGCCGTTTCATTTCGCGCAGCGACTGTTAGGTGA
CATGGTAAAATATCTCGACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTPSAVSKLVSRLERRLGTRLFNRSTRQLQLTPEGAAFYDRSVRILADLAEAEQCASASALPHGRVRINVNVPMGRRFLL
PLVPALSARYPDLVLDIVLTDTVVDLMEDRTDIAIRHGPLKSSRLMARKLGRMRMRLVAAPAYLARYGMPGTPADLRRHR
RIGSSYWRMHDGWPQLDDGGPVPVLPPGIVQASDGDAVLQLAIAGMGLAYLAAFQVDEHIAAGKLVPVLEAFHSPALVDT
HAVYLGHHGKLPARIRAVLDFLQENMRIGNGEGMVAEHGRVAASWMADG

Sequences:

>Translated_289_residues
MTPSAVSKLVSRLERRLGTRLFNRSTRQLQLTPEGAAFYDRSVRILADLAEAEQCASASALPHGRVRINVNVPMGRRFLL
PLVPALSARYPDLVLDIVLTDTVVDLMEDRTDIAIRHGPLKSSRLMARKLGRMRMRLVAAPAYLARYGMPGTPADLRRHR
RIGSSYWRMHDGWPQLDDGGPVPVLPPGIVQASDGDAVLQLAIAGMGLAYLAAFQVDEHIAAGKLVPVLEAFHSPALVDT
HAVYLGHHGKLPARIRAVLDFLQENMRIGNGEGMVAEHGRVAASWMADG
>Mature_288_residues
TPSAVSKLVSRLERRLGTRLFNRSTRQLQLTPEGAAFYDRSVRILADLAEAEQCASASALPHGRVRINVNVPMGRRFLLP
LVPALSARYPDLVLDIVLTDTVVDLMEDRTDIAIRHGPLKSSRLMARKLGRMRMRLVAAPAYLARYGMPGTPADLRRHRR
IGSSYWRMHDGWPQLDDGGPVPVLPPGIVQASDGDAVLQLAIAGMGLAYLAAFQVDEHIAAGKLVPVLEAFHSPALVDTH
AVYLGHHGKLPARIRAVLDFLQENMRIGNGEGMVAEHGRVAASWMADG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=269, Percent_Identity=35.6877323420074, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789639, Length=283, Percent_Identity=31.095406360424, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081978, Length=272, Percent_Identity=30.1470588235294, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI145693193, Length=236, Percent_Identity=30.0847457627119, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787128, Length=273, Percent_Identity=27.4725274725275, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789440, Length=245, Percent_Identity=30.6122448979592, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=29.6875, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=28.515625, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31623; Mature: 31492

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSAVSKLVSRLERRLGTRLFNRSTRQLQLTPEGAAFYDRSVRILADLAEAEQCASASA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LPHGRVRINVNVPMGRRFLLPLVPALSARYPDLVLDIVLTDTVVDLMEDRTDIAIRHGPL
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KSSRLMARKLGRMRMRLVAAPAYLARYGMPGTPADLRRHRRIGSSYWRMHDGWPQLDDGG
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCC
PVPVLPPGIVQASDGDAVLQLAIAGMGLAYLAAFQVDEHIAAGKLVPVLEAFHSPALVDT
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEE
HAVYLGHHGKLPARIRAVLDFLQENMRIGNGEGMVAEHGRVAASWMADG
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCC
>Mature Secondary Structure 
TPSAVSKLVSRLERRLGTRLFNRSTRQLQLTPEGAAFYDRSVRILADLAEAEQCASASA
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LPHGRVRINVNVPMGRRFLLPLVPALSARYPDLVLDIVLTDTVVDLMEDRTDIAIRHGPL
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KSSRLMARKLGRMRMRLVAAPAYLARYGMPGTPADLRRHRRIGSSYWRMHDGWPQLDDGG
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCC
PVPVLPPGIVQASDGDAVLQLAIAGMGLAYLAAFQVDEHIAAGKLVPVLEAFHSPALVDT
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEE
HAVYLGHHGKLPARIRAVLDFLQENMRIGNGEGMVAEHGRVAASWMADG
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]