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The map label for this gene is hisH [H]

Identifier: 162145909

GI number: 162145909

Start: 77032

End: 77703

Strand: Reverse

Name: hisH [H]

Synonym: GDI_0071

Alternate gene names: 162145909

Gene position: 77703-77032 (Counterclockwise)

Preceding gene: 162145910

Following gene: 162145908

Centisome position: 1.97

GC content: 70.09

Gene sequence:

>672_bases
ATGGCGACCGCGACGCAATCCATCGTAGTGATCGACTACAACGGCGGCAACCTGGCATCCGCCGCGCGGGCGGTGGCGCG
CGCCGCCGCCGATAGCGGGATCGACGCCACCGTGACGATCACCGCCGACCCCGAGTCGGTGCGCGGGGCGGACCGGATCG
TGCTGCCTGGACAGGGTGCGTTCGCCGATTGCGCGGCGGGGCTGGCGGCGGTGCCCGGGCTGAAGCCCGCGATCATCGAC
GCGACCGAAGCCGGCACGCCGTTCCTGGGCATCTGCGTGGGTATGCAACTGATGGCCCAGCGCGGGCTGGAACACGCAGT
GACGCCGGGATTCGGCTGGATTTCCGGCGAGATCGCGCGGATGGAGGCACCGGGACTGCGCCTGCCGCAGATGGGCTGGA
ACCGGCTGGATTTCACACCGGGCGCGCATCCGCTGACCGAGGGGCTGGACGCCGACGCCCACGGCTATTTCGTGCATTCC
TACGCGCTGCGTCACGGGGCGGTGGACGAACTGGTGGCAACGACCGACTATGGCGGGGCGGTGCCGGCGATCGTGGCCCG
GGGCAATCGCGCCGGCACCCAGTTCCATGTCGAGAAGAGCCAGACGGTCGGCCTGCGGATCCTGTCGAATTTCCTGCGCT
GGTCGCCCGTTCGCGAAGGAGCCGCCGCATGA

Upstream 100 bases:

>100_bases
CGGTGGTGCTGGCGCGCGGACGGGATGCCGCGCTGCTGCGCCTGCTGGACCGCCACCCCGAGATCGCGACCCGGCACCGG
CCGGCGGCGGAGGCATGAGC

Downstream 100 bases:

>100_bases
ACGAGATCGGCACGCCGTCCCAGACCCGCCCCCGCGCCGAACGGGTGCAGGCCCTGCATGAAGACGATCTGCAGGCGCTG
TGCGAGGCCACCGACGCCGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MATATQSIVVIDYNGGNLASAARAVARAAADSGIDATVTITADPESVRGADRIVLPGQGAFADCAAGLAAVPGLKPAIID
ATEAGTPFLGICVGMQLMAQRGLEHAVTPGFGWISGEIARMEAPGLRLPQMGWNRLDFTPGAHPLTEGLDADAHGYFVHS
YALRHGAVDELVATTDYGGAVPAIVARGNRAGTQFHVEKSQTVGLRILSNFLRWSPVREGAAA

Sequences:

>Translated_223_residues
MATATQSIVVIDYNGGNLASAARAVARAAADSGIDATVTITADPESVRGADRIVLPGQGAFADCAAGLAAVPGLKPAIID
ATEAGTPFLGICVGMQLMAQRGLEHAVTPGFGWISGEIARMEAPGLRLPQMGWNRLDFTPGAHPLTEGLDADAHGYFVHS
YALRHGAVDELVATTDYGGAVPAIVARGNRAGTQFHVEKSQTVGLRILSNFLRWSPVREGAAA
>Mature_222_residues
ATATQSIVVIDYNGGNLASAARAVARAAADSGIDATVTITADPESVRGADRIVLPGQGAFADCAAGLAAVPGLKPAIIDA
TEAGTPFLGICVGMQLMAQRGLEHAVTPGFGWISGEIARMEAPGLRLPQMGWNRLDFTPGAHPLTEGLDADAHGYFVHSY
ALRHGAVDELVATTDYGGAVPAIVARGNRAGTQFHVEKSQTVGLRILSNFLRWSPVREGAAA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=209, Percent_Identity=34.4497607655502, Blast_Score=109, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=30.9859154929577, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23042; Mature: 22911

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATATQSIVVIDYNGGNLASAARAVARAAADSGIDATVTITADPESVRGADRIVLPGQGA
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCEEEECCCCC
FADCAAGLAAVPGLKPAIIDATEAGTPFLGICVGMQLMAQRGLEHAVTPGFGWISGEIAR
HHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
MEAPGLRLPQMGWNRLDFTPGAHPLTEGLDADAHGYFVHSYALRHGAVDELVATTDYGGA
ECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHCCCHHHHHEECCCCCC
VPAIVARGNRAGTQFHVEKSQTVGLRILSNFLRWSPVREGAAA
CCCEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ATATQSIVVIDYNGGNLASAARAVARAAADSGIDATVTITADPESVRGADRIVLPGQGA
CCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCEEEECCCCC
FADCAAGLAAVPGLKPAIIDATEAGTPFLGICVGMQLMAQRGLEHAVTPGFGWISGEIAR
HHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
MEAPGLRLPQMGWNRLDFTPGAHPLTEGLDADAHGYFVHSYALRHGAVDELVATTDYGGA
ECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHCCCHHHHHEECCCCCC
VPAIVARGNRAGTQFHVEKSQTVGLRILSNFLRWSPVREGAAA
CCCEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA