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The map label for this gene is hisA [H]

Identifier: 162145908

GI number: 162145908

Start: 75680

End: 77035

Strand: Reverse

Name: hisA [H]

Synonym: GDI_0070

Alternate gene names: 162145908

Gene position: 77035-75680 (Counterclockwise)

Preceding gene: 162145909

Following gene: 162145907

Centisome position: 1.95

GC content: 70.28

Gene sequence:

>1356_bases
ATGAACGAGATCGGCACGCCGTCCCAGACCCGCCCCCGCGCCGAACGGGTGCAGGCCCTGCATGAAGACGATCTGCAGGC
GCTGTGCGAGGCCACCGACGCCGCGATCCTGGATGGCGGGGGGTTCGGCTGGCTGACCACGCCCGGCCGACAGGCGATGG
AGCGCTATTTTCGCGGCGTGCTGATGGTGCCGGAGCGCATGCTGTTCGTGGTCCGGCTGGACGGCATCATCGTGGGTGCC
GCCCAACTGGTGCGCCCGCCGCGCAATAACGAGGCGCAGGCCATGAGCGCCACGCTGATGCACCTGTATGTCGCGCCCTA
TGCCAGGGGCCTGGGGCTGGGCCGGCTGCTGCTGCTGGAGGCCGAGCAGTGCGCGCGGGCCATGGGGTACCAGATCCTGA
ACCTGGACGTGCGCGAGACGCAGGAATCGGCCATCCGGCTGTTCCGCGCCTTCGGCTTCCATCACTGGGGCACCCATCCC
AGCTATGCCCGCACGGAGGGGCGCACGGTGCGGGGCCTGTTCTTCACCAAGCGCCTGCAGGACAATGAACGGGTGGTACC
CGCGCATCCGCAGGCCGCTTCCATTCCCATTCCTGCCACGGGACCCGCCAGCGTGACCGGACACAGCCTGACCCTGTACC
CCGCCATCGACCTGAAGGACGGGGCCTGCGTGCGCCTGCGCCGGGGCGAGATGGACGATGCCACGATCTATTCCGACAAT
CCGGGCGCGCAGGCCCGTGCCTGGGTGCAGGCGGGTTGCCGGTGGCTGCATGTCGTGGACCTGAACGGTGCATTCGCCGG
CCGGTCGGCCAATGGCGACGCGGTCGAGGCGATCATCGCCAACGCCACCGTGCCGGTGCAGCTGGGCGGCGGTCTGCGGG
ACATGGCGGGCATCGAACGCTGGCTGGCCGCCGGTGTCACGCGCGTCATCCTGGGCAGCGTCGCGGTCAAGGACCCCGAA
CTGGTGCGTGCGGCCTGCCGCGCCTTCCCCGGCCGGATCGTGGCGGGGATCGACGCGCGATCGGGCCAGGTGGCGACCGA
GGGCTGGGCCGAGACGTCGGACATGAAGGCGGTCGAACTGGCCCGCCGGATGGAGGACGTGGGGGTGGCCGCGATCATCT
TCACCGAGATCAGCCGCGACGGCATGCTGACGGGCATCGACATCGCCCAGACCGTCGAGATGGCGAATGCGCTGTCGATC
CCGGTCATCGCCAGCGGCGGAGTCGGCCATGCCGACCACCTGCATGCCCTGCGTGCCGCGACGGTGCAGGCACCGGGGAT
CGAGGGCGTGATCGTCGGCCGGGCGCTGTATGACGGCCGGGTGGACCCGGCCGAGGCCTTGCGGATCCTGTCCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCACCCAGTTCCATGTCGAGAAGAGCCAGACGGTCGGCCTGCGGATCCTGTCGAATTTCCTGCGCTGGTCGCCC
GTTCGCGAAGGAGCCGCCGC

Downstream 100 bases:

>100_bases
TGCTGAAGCTGCGGGTCATCCCCTGTCTGGACGTCAAGAACGGGCGCGTCGTGAAGGGCGTCAACTTCGTATCGCTGCGC
GATGCCGGGGACCCGGTGGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNEIGTPSQTRPRAERVQALHEDDLQALCEATDAAILDGGGFGWLTTPGRQAMERYFRGVLMVPERMLFVVRLDGIIVGA
AQLVRPPRNNEAQAMSATLMHLYVAPYARGLGLGRLLLLEAEQCARAMGYQILNLDVRETQESAIRLFRAFGFHHWGTHP
SYARTEGRTVRGLFFTKRLQDNERVVPAHPQAASIPIPATGPASVTGHSLTLYPAIDLKDGACVRLRRGEMDDATIYSDN
PGAQARAWVQAGCRWLHVVDLNGAFAGRSANGDAVEAIIANATVPVQLGGGLRDMAGIERWLAAGVTRVILGSVAVKDPE
LVRAACRAFPGRIVAGIDARSGQVATEGWAETSDMKAVELARRMEDVGVAAIIFTEISRDGMLTGIDIAQTVEMANALSI
PVIASGGVGHADHLHALRAATVQAPGIEGVIVGRALYDGRVDPAEALRILS

Sequences:

>Translated_451_residues
MNEIGTPSQTRPRAERVQALHEDDLQALCEATDAAILDGGGFGWLTTPGRQAMERYFRGVLMVPERMLFVVRLDGIIVGA
AQLVRPPRNNEAQAMSATLMHLYVAPYARGLGLGRLLLLEAEQCARAMGYQILNLDVRETQESAIRLFRAFGFHHWGTHP
SYARTEGRTVRGLFFTKRLQDNERVVPAHPQAASIPIPATGPASVTGHSLTLYPAIDLKDGACVRLRRGEMDDATIYSDN
PGAQARAWVQAGCRWLHVVDLNGAFAGRSANGDAVEAIIANATVPVQLGGGLRDMAGIERWLAAGVTRVILGSVAVKDPE
LVRAACRAFPGRIVAGIDARSGQVATEGWAETSDMKAVELARRMEDVGVAAIIFTEISRDGMLTGIDIAQTVEMANALSI
PVIASGGVGHADHLHALRAATVQAPGIEGVIVGRALYDGRVDPAEALRILS
>Mature_451_residues
MNEIGTPSQTRPRAERVQALHEDDLQALCEATDAAILDGGGFGWLTTPGRQAMERYFRGVLMVPERMLFVVRLDGIIVGA
AQLVRPPRNNEAQAMSATLMHLYVAPYARGLGLGRLLLLEAEQCARAMGYQILNLDVRETQESAIRLFRAFGFHHWGTHP
SYARTEGRTVRGLFFTKRLQDNERVVPAHPQAASIPIPATGPASVTGHSLTLYPAIDLKDGACVRLRRGEMDDATIYSDN
PGAQARAWVQAGCRWLHVVDLNGAFAGRSANGDAVEAIIANATVPVQLGGGLRDMAGIERWLAAGVTRVILGSVAVKDPE
LVRAACRAFPGRIVAGIDARSGQVATEGWAETSDMKAVELARRMEDVGVAAIIFTEISRDGMLTGIDIAQTVEMANALSI
PVIASGGVGHADHLHALRAATVQAPGIEGVIVGRALYDGRVDPAEALRILS

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=34.9794238683128, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788336, Length=226, Percent_Identity=24.3362831858407, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 48339; Mature: 48339

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIGTPSQTRPRAERVQALHEDDLQALCEATDAAILDGGGFGWLTTPGRQAMERYFRGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCE
LMVPERMLFVVRLDGIIVGAAQLVRPPRNNEAQAMSATLMHLYVAPYARGLGLGRLLLLE
EECCCCEEEEEEECCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AEQCARAMGYQILNLDVRETQESAIRLFRAFGFHHWGTHPSYARTEGRTVRGLFFTKRLQ
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHCC
DNERVVPAHPQAASIPIPATGPASVTGHSLTLYPAIDLKDGACVRLRRGEMDDATIYSDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEEECCC
PGAQARAWVQAGCRWLHVVDLNGAFAGRSANGDAVEAIIANATVPVQLGGGLRDMAGIER
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHHHH
WLAAGVTRVILGSVAVKDPELVRAACRAFPGRIVAGIDARSGQVATEGWAETSDMKAVEL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCCHHHHHH
ARRMEDVGVAAIIFTEISRDGMLTGIDIAQTVEMANALSIPVIASGGVGHADHLHALRAA
HHHHHHCCEEEEEEEECCCCCCEECCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
TVQAPGIEGVIVGRALYDGRVDPAEALRILS
HCCCCCCCEEEEEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MNEIGTPSQTRPRAERVQALHEDDLQALCEATDAAILDGGGFGWLTTPGRQAMERYFRGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCE
LMVPERMLFVVRLDGIIVGAAQLVRPPRNNEAQAMSATLMHLYVAPYARGLGLGRLLLLE
EECCCCEEEEEEECCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AEQCARAMGYQILNLDVRETQESAIRLFRAFGFHHWGTHPSYARTEGRTVRGLFFTKRLQ
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHCC
DNERVVPAHPQAASIPIPATGPASVTGHSLTLYPAIDLKDGACVRLRRGEMDDATIYSDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCEEECCC
PGAQARAWVQAGCRWLHVVDLNGAFAGRSANGDAVEAIIANATVPVQLGGGLRDMAGIER
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECCCHHHHHHHHH
WLAAGVTRVILGSVAVKDPELVRAACRAFPGRIVAGIDARSGQVATEGWAETSDMKAVEL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCCHHHHHH
ARRMEDVGVAAIIFTEISRDGMLTGIDIAQTVEMANALSIPVIASGGVGHADHLHALRAA
HHHHHHCCEEEEEEEECCCCCCEECCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
TVQAPGIEGVIVGRALYDGRVDPAEALRILS
HCCCCCCCEEEEEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA