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The map label for this gene is pncA [H]

Identifier: 161871020

GI number: 161871020

Start: 2147570

End: 2148082

Strand: Reverse

Name: pncA [H]

Synonym: NMCC_2116

Alternate gene names: 161871020

Gene position: 2148082-2147570 (Counterclockwise)

Preceding gene: 161871022

Following gene: 161871019

Centisome position: 99.75

GC content: 46.0

Gene sequence:

>513_bases
TTGCGCGTGATGACGAAAGATGCGCATCATGCGGCAGCAAAATGGCTTGTGGATAACCCTGTTGATATGTTGAAGCCGAC
AGGTTTGCCCGATGCGGATTTGACTTGGGTGGCTCATGCGATGGTTGGTACGCGCGGCTATGAATTGTTGGACGGGCTGC
CTTGTGCCAAAGAATACGATTATTGCGTTTGGAAAGGTGTTGATCCTGAATTGCATCCTTACGGCGCGTGTTTTCACGAT
ATTGAGGAAAAACTAAGCACAGGGCTGATTGAATGGCTGCGTTGTCAAAATACGGATACGGTTATTATCGGTGGGTTAGC
TACGGATTATTGTGTTAAAACAACGGTTTTGCAGTTACTCAAAGGCGGTCGTTGGCAGGTTGTCGTCAATGAAGCGGCTT
GTCGGGGTATTGCGCCGGAAACCATCGAAACAGCATGGCAGGAAATGCATTCTGCCGGGGCAATCATCTTAAAAAACGCT
GATGAAATAAATAAATATATTAATAATCAATAA

Upstream 100 bases:

>100_bases
TGCACAAAAAACTTTTACGCCTTTGTGTCCTAAAGAGCTGCCTGTAAACGAGGGGCATTTGATTGTCGAAGAATTGAATG
CCCAAGCCGCTTTGGCGGAT

Downstream 100 bases:

>100_bases
GTTAAAATTGTTTCACGTGAAACCCCCTTCCTAAATATGAAAATTTTGCTTGTCCGCTTGTCTAGTATGGGCGATTTAAT
TCACACTTTGCCCGCAATCG

Product: pyrazinamidase/nicotinamidase PncA, putative

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MRVMTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDYCVWKGVDPELHPYGACFHD
IEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQLLKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNA
DEINKYINNQ

Sequences:

>Translated_170_residues
MRVMTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDYCVWKGVDPELHPYGACFHD
IEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQLLKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNA
DEINKYINNQ
>Mature_170_residues
MRVMTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDYCVWKGVDPELHPYGACFHD
IEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQLLKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNA
DEINKYINNQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=134, Percent_Identity=38.8059701492537, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 18911; Mature: 18911

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYD
CCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCC
YCVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQLL
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
KGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ
CCCCEEEEEECHHHCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCC
>Mature Secondary Structure
MRVMTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYD
CCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCC
YCVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQLL
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
KGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ
CCCCEEEEEECHHHCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]