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The map label for this gene is nusA [H]

Identifier: 161870486

GI number: 161870486

Start: 1570295

End: 1571785

Strand: Direct

Name: nusA [H]

Synonym: NMCC_1542

Alternate gene names: 161870486

Gene position: 1570295-1571785 (Clockwise)

Preceding gene: 161870485

Following gene: 161870487

Centisome position: 72.92

GC content: 57.08

Gene sequence:

>1491_bases
ATGTTACAGCTGGCAGAAGCACTGGCAAGCGAAAAAAACGTTGATGCCGAAGTCGTCTTCCAAGCACTGGAATTCGCCCT
GTCTACCGCCGCCAAGAAAAAGGCAGACCGCGAACACATGGACGTGCGCGTACAAATCAACCGCGATACCGGCGAATACC
AAACCTTCCGCCGCTGGCTGATTGTCGCCGATGAAGACTATACCTATCCCGATGTCGAAAAAACCATCGAGGAAATCCAA
GAGGAAATTCCCGGCATCGCCATCCAAATCGGCGAATACTATGAAGAGCAGCTGCCCAACGAAGGCTTCGGCCGCCAAGC
CGCGCAAACCGCCAAACAAATCATCCTGCAACGCATCCGCGATGCCGAGCGCGAGCAGAATCTGAACGAGTTTCTCGCCG
TCAAAGAAGACATCGTGTCCGGCACGGTCAAACGCGTCGAACGCCACGGCATCATCGTCGAAGTCGTTGCCGGCAAACTG
GACGCGCTGATTCCGCGCGACCAAATGATTCCGCGCGAAAACTTCCGCAGCGGCGACCGCATCCGCGCCCTCTTCCTGCG
CGTCGAAGAAATCGGCAACACCGGCCGCAAACAAGTCATCCTGAGCCGTACTTCCGGCGATTTCCTCGTCAAACTGTACG
CCAATGAAGTACCTGAAATTGCAGACGGCATGCTTGAAATCCGCGCTGTCGCCCGCGACCCGGGACAACGTGCCAAAGTC
GCCGTCAAAGCCAACGACCAGCGCATCGATCCGCAAGGCACCTGTATCGGCGTTCGCGGTTCGCGTGTCAATGCCGTCAG
CAACGAATTGTCCGGTGAGCGCATCGACGTTGTCCTTTGGTCGCCCGAGCCTGCCCAATTCGTCATGAGCGCGCTCTCAC
CCGCCGAAGTCAGCCGCATCGTCATCGACGAAGACAAACACGCCGTCGATGTCATCGTTGCCGAAGACCAGCTCGCGCTC
GCCATCGGACGCGGCGGTCAAAACGTGCGCCTTGCTTCCGAGCTGACCGGCTGGCAGCTCAACATCATGACTTCCGCCGA
GGCAGACGAACGCAATGCGGCAGAAGATGCCGCCATCCGCCGCCTGTTTATGGATCACTTGAACGTGGACGAAGAAACCG
CCGACGTACTGGTTCAGGAAGGTTTTGCAACCTTGGAAGAAGTCGCCTATGTTCCTGCCGCCGAACTGCTTGCCATTGAA
GGATTTGACGAAGAAATCGTCGATATGCTCCGCAACCGCGCCCGCGATGCCATCCTGACCATGGCGATTGCCGCCGAAGA
AAAACTGGGCGAAGTGTCCGACGATATGCGCAACCTCGAAGGTATAGATGCCGATATGCTCCGCAGCCTTGCCGAAGCAG
GCATTACCACCCGCGACGACTTGGCAGAGCTTGCTGTGGACGAACTGATTGAAATCACCGGTGTAAACGAAGAAACCGCA
AAAGCCGTCATCCTGACCGCACGCGAACACTGGTTTACCGAAGACAAATAA

Upstream 100 bases:

>100_bases
CGTGCAAATCGGGTTGGACAACATCGACAAAGCCCGCCTGCGCCCCGAATTCAAATTCTAAAACACAACAATATTGGAGA
TGTTCAAAATGAGTCGTGAA

Downstream 100 bases:

>100_bases
AGGGGGTACAGATGAGTAACACAACCGTAGAACAATTTGCCGCCGAGCTGAAACGCCCCGTCGAAGACCTGTTGAAACAG
TTGAAAGAAGCCGGCGTCAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHMDVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQ
EEIPGIAIQIGEYYEEQLPNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVVAGKL
DALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYANEVPEIADGMLEIRAVARDPGQRAKV
AVKANDQRIDPQGTCIGVRGSRVNAVSNELSGERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLAL
AIGRGGQNVRLASELTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEVAYVPAAELLAIE
GFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADMLRSLAEAGITTRDDLAELAVDELIEITGVNEETA
KAVILTAREHWFTEDK

Sequences:

>Translated_496_residues
MLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHMDVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQ
EEIPGIAIQIGEYYEEQLPNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVVAGKL
DALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYANEVPEIADGMLEIRAVARDPGQRAKV
AVKANDQRIDPQGTCIGVRGSRVNAVSNELSGERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLAL
AIGRGGQNVRLASELTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEVAYVPAAELLAIE
GFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADMLRSLAEAGITTRDDLAELAVDELIEITGVNEETA
KAVILTAREHWFTEDK
>Mature_496_residues
MLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHMDVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQ
EEIPGIAIQIGEYYEEQLPNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVVAGKL
DALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYANEVPEIADGMLEIRAVARDPGQRAKV
AVKANDQRIDPQGTCIGVRGSRVNAVSNELSGERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLAL
AIGRGGQNVRLASELTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEVAYVPAAELLAIE
GFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADMLRSLAEAGITTRDDLAELAVDELIEITGVNEETA
KAVILTAREHWFTEDK

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=48.0808080808081, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55245; Mature: 55245

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHMDVRVQINRDTGEYQTFRRWL
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHEEEE
IVADEDYTYPDVEKTIEEIQEEIPGIAIQIGEYYEEQLPNEGFGRQAAQTAKQIILQRIR
EEECCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVVAGKLDALIPRDQMIPRENFRSGDR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHCCCCHHCCCHHHCCCCHH
IRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYANEVPEIADGMLEIRAVARDPGQRAKV
HHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
AVKANDQRIDPQGTCIGVRGSRVNAVSNELSGERIDVVLWSPEPAQFVMSALSPAEVSRI
EEECCCCEECCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHEEE
VIDEDKHAVDVIVAEDQLALAIGRGGQNVRLASELTGWQLNIMTSAEADERNAAEDAAIR
EEECCCCEEEEEEECCCEEEEECCCCCCEEEHHHCCCEEEEEEECCCCCCCCHHHHHHHH
RLFMDHLNVDEETADVLVQEGFATLEEVAYVPAAELLAIEGFDEEIVDMLRNRARDAILT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHHHHHH
MAIAAEEKLGEVSDDMRNLEGIDADMLRSLAEAGITTRDDLAELAVDELIEITGVNEETA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHC
KAVILTAREHWFTEDK
CEEEEEEHHHCCCCCC
>Mature Secondary Structure
MLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHMDVRVQINRDTGEYQTFRRWL
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHEEEE
IVADEDYTYPDVEKTIEEIQEEIPGIAIQIGEYYEEQLPNEGFGRQAAQTAKQIILQRIR
EEECCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVVAGKLDALIPRDQMIPRENFRSGDR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHCCCCHHCCCHHHCCCCHH
IRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYANEVPEIADGMLEIRAVARDPGQRAKV
HHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEE
AVKANDQRIDPQGTCIGVRGSRVNAVSNELSGERIDVVLWSPEPAQFVMSALSPAEVSRI
EEECCCCEECCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHEEE
VIDEDKHAVDVIVAEDQLALAIGRGGQNVRLASELTGWQLNIMTSAEADERNAAEDAAIR
EEECCCCEEEEEEECCCEEEEECCCCCCEEEHHHCCCEEEEEEECCCCCCCCHHHHHHHH
RLFMDHLNVDEETADVLVQEGFATLEEVAYVPAAELLAIEGFDEEIVDMLRNRARDAILT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCHHHHHHHHHHHHHHHHH
MAIAAEEKLGEVSDDMRNLEGIDADMLRSLAEAGITTRDDLAELAVDELIEITGVNEETA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHC
KAVILTAREHWFTEDK
CEEEEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]