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The map label for this gene is potD3 [H]

Identifier: 161870449

GI number: 161870449

Start: 1525393

End: 1526523

Strand: Reverse

Name: potD3 [H]

Synonym: NMCC_1504

Alternate gene names: 161870449

Gene position: 1526523-1525393 (Counterclockwise)

Preceding gene: 161870454

Following gene: 161870445

Centisome position: 70.89

GC content: 51.11

Gene sequence:

>1131_bases
ATGGCCAAACATCTGCCACTCGCCGTCCTGACTGCTTTGCTGCTTGCAGCGTGCGGCGGTTCGGACAAACCATCTGCCGA
AAATCCGACATCGGCGGAAAACCGAAACGTATTAAAAATTTACAACTGGTCGGAATATGTCGATCCGGAAACCGTTGCCG
ATTTTGAAAAGAAAAACGGCATCAAGGTTACTTATGATGTGTACGACAGCGATGAAACGCTGGAAAGCAAGGTGCTGACA
GGCAAGTCCGGTTACGACATTGTCGCGCCGTCCAATGCGTTTGTGGGCAGGCAGATTAAGGCAGGTGCGTATCAGAAAAT
CGATAAGTCGCTGATTCCCAATTATAAACACCTCAACCCCGAAATGATGAGGCTGATGGACGGGGTCGATCCCGGCCACG
AATACGCCGTGCCGTTTTATTGGGGGACAAATACCTTCGCCATCAATACCGAACGCGTGAAAAAGGCTTTGGGTACGGAC
AAGCTGCCGGACAACCAGTGGGATTTGGTGTTCGACCCCGAATACACGTCCAAACTCAAGCAATGCGGCATCAGCTATTT
GGACAGCGCGGCGGAAATCTATCCTATGGTGTTGAACTATTTGGGTAAAAACCCGAACAGCAGCAATACGGAAGACATCA
GGGAGGCAACCGCCCTACTCAAGAAAAACCGCCCCAATATCAAACGCTTTACTTCGTCCGGCTTTATCGATGATTTGGCG
CGCGGCGATACCTGCGTAACAATCGGTTTCGGCGGCGATTTGAACATCGCCAAACGCCGTGCCGAAGAAGCGGGCGGCAA
GGAAAAAATCCGCGTGATGATGCCCAAAGAGGGCGTGGGGATTTGGGTGGATTCTTTCGTGATTCCGAAAGATGCGAAAA
ACGTCGCCAACGCGCACAAATACATCAACGACTTCCTCGACCCGGAAGTGTCGGCGAAAAACGGCAATTTCGTTACTTAT
GCGCCTTCGAGCAAGCCGGCGCGTGAGCTGATGGAAGACGAATTTAAAAACGACAATACGATTTTCCCAACCGAGGAGGA
TTTGAAAAACAGCTTTATCATGGTGCCTATCCAGCCGGCGGCATTGAAGTTTATGGTGCGCCAGTGGCAGGATGTGAAGG
CGGGGAAATAA

Upstream 100 bases:

>100_bases
TTCGGAAGGGTGTGGCGGGATTTTGAATGCCGTCTGAAGCCCTGTTGCCAATATCGGCTATAATGACCGCTTTCTCCAAC
CCGATATGCAAGGAATGATA

Downstream 100 bases:

>100_bases
AGCCCGATATGCCGTCTGAAGGATGTTCGGACGGCATTTTTTATCTTTGGCGGAACAGGGATTGCAGCCGCTGTTTGAAG
GCAGTGGGGCGGATGCTGCT

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWSEYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLT
GKSGYDIVAPSNAFVGRQIKAGAYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLA
RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTY
APSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK

Sequences:

>Translated_376_residues
MAKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWSEYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLT
GKSGYDIVAPSNAFVGRQIKAGAYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLA
RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTY
APSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK
>Mature_375_residues
AKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWSEYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTG
KSGYDIVAPSNAFVGRQIKAGAYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTDK
LPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLAR
GDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYA
PSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=348, Percent_Identity=43.1034482758621, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI1787367, Length=346, Percent_Identity=34.1040462427746, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787711, Length=331, Percent_Identity=24.773413897281, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41916; Mature: 41785

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWSEYVDPETVADFEKKNG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHCCC
IKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAGAYQKIDKSLIPNYKHLNP
CEEEEEECCCCHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCH
EMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTDKLPDNQWDLVFDPEYTSKLK
HHHHHHHCCCCCCCEEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCEEECCHHHHHHH
QCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLA
HHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHH
RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHK
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECEECCCCHHHHHHHHH
YINDFLDPEVSAKNGNFVTYAPSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPA
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCHH
ALKFMVRQWQDVKAGK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWSEYVDPETVADFEKKNG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHCCC
IKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAGAYQKIDKSLIPNYKHLNP
CEEEEEECCCCHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCH
EMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTDKLPDNQWDLVFDPEYTSKLK
HHHHHHHCCCCCCCEEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCEEECCHHHHHHH
QCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLA
HHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHH
RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHK
CCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEECEECCCCHHHHHHHHH
YINDFLDPEVSAKNGNFVTYAPSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPA
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCHH
ALKFMVRQWQDVKAGK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]