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The map label for this gene is smpB [H]

Identifier: 161870386

GI number: 161870386

Start: 1454293

End: 1454739

Strand: Reverse

Name: smpB [H]

Synonym: NMCC_1432

Alternate gene names: 161870386

Gene position: 1454739-1454293 (Counterclockwise)

Preceding gene: 161870387

Following gene: 161870382

Centisome position: 67.55

GC content: 47.2

Gene sequence:

>447_bases
ATGGCTATTGCGAACAATAAAAAAGCATTTCACGATTTTTTCATTGAAGACCGGATTGAAGCCGGTTTGGTCTTGGAAGG
CTGGGAAGTCAAAGCAATCCGCGCCGCGCGCGTACAGCTTAAAGAGAGTTATATCTATTGGAAAAAAGACGCGTTTTATC
TGGTCGGCTGCCATATTACGGCTTTGCCCACAGCCTCGACACACATTAAACCAGATGCCGTACGTCCGCGCAAGCTCTTG
TTGAACCAGTCGGAAATCAACAAATTAATCGGCAAAACCGAACGTGCCGGCTACACCATCGTGCCGCTGGATTTGCATTT
TTCGCGCGGAAAAATCAAGATGGAAATCGGTTTGGCAAAAGGTAAGAAACAGCACGACAAACGTCAGAGTATGAAAGAGG
CGGATTGGAAACGCGAGAAACAGCGGTTGATTAAGCATACGCGCTGA

Upstream 100 bases:

>100_bases
GCTGTATCCCGAGAAGATTGTGCAGGCGGTTGAAGAGGCGGTATGATGGGTTCTGTTTTCGGGTTTATGCCGTCTGACGG
CGGATATTGATAGGATGTTT

Downstream 100 bases:

>100_bases
TGGGGGGGATGCGGTTTCAGACGGCGTTTGAACAATGCCGTCTGACGGTTGTTTGAACAATGGTGTTGAAATACGAAACG
GAAAAACAGGGCGGTTGCCC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESYIYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLL
LNQSEINKLIGKTERAGYTIVPLDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR

Sequences:

>Translated_148_residues
MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESYIYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLL
LNQSEINKLIGKTERAGYTIVPLDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR
>Mature_147_residues
AIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESYIYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLL
NQSEINKLIGKTERAGYTIVPLDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=47.9166666666667, Blast_Score=149, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17179; Mature: 17048

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESYIYWKKDAFYLVGCHIT
CCCCCCCCHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHHCEEEEECCCEEEEEEEEE
ALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVPLDLHFSRGKIKMEIGLAK
ECCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEEEECCC
GKKQHDKRQSMKEADWKREKQRLIKHTR
CCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESYIYWKKDAFYLVGCHIT
CCCCCCCHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHHHCEEEEECCCEEEEEEEEE
ALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVPLDLHFSRGKIKMEIGLAK
ECCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEEEECCC
GKKQHDKRQSMKEADWKREKQRLIKHTR
CCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA