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The map label for this gene is rfaF [H]

Identifier: 161870387

GI number: 161870387

Start: 1454794

End: 1455753

Strand: Reverse

Name: rfaF [H]

Synonym: NMCC_1433

Alternate gene names: 161870387

Gene position: 1455753-1454794 (Counterclockwise)

Preceding gene: 161870388

Following gene: 161870386

Centisome position: 67.6

GC content: 54.27

Gene sequence:

>960_bases
ATGACCCAGCCCTTGTTCCGCCGTTTGAAGGAACTTCACCCCGGTTGCACGATTGATGTGTTCGCACCGAAGTGGTCGAT
GGCGGTGTTTGAGCGTATGCCGGAAGTGAATGAGATTCTTGAAAATCCGTTCGGACATGGTGCGTTGGAGTTGAAACGCC
GCTGGCGGGTCGGTAGGGATTTGGGGCGGCGCGGATACGATCAGGTTATCGTGTTGCCCGGTTCTTTGAAATCGGCAATC
ATCGCGCTGGCGACAGGTATCGGTAAAAGGACGGGTTATGTCGGTGAAAGCCGTTATTTTCTGTTGAACGATATACGCAG
GCTGGATAAGGAACGTCTGCCTTTGATGGTGGACAGATATACGGCTCTCGCGCATCCGAGCCAGGAGGATTTTGACGGGC
ATTCGGGATTCCCCGAGTTTTCCATTGATGAACGGCGGCGGGAAATTTCTGTCGAAACCTTTGGCTTGGATATTGGAAAG
CCTGTTCTGGCTTTTTGTCCGGGTGCGGAATTCGGGCCGGCAAAGCGTTGGCCGACAAGGTATTTTGCCGAGTTGGGCAA
ACATTATTCGGCGGCGGGTTGGCAGGTTTGGCTGTTCGGTTCGCAAAAAGATGATGAAATTGCCGAGGAAATCAACTGCC
TTTCAGACGGTATGTGTGTGAATTTATGCGGAAAGACCGATTTGTCGCAGGCAATGGATTTGCTGTCGTTGGCGGACACG
GTCGTGTGCAACGACAGCGGGCTGATGCACTTGGCGGCGGCATTGGGCAGGAAGGTGGTGGCGGTTTACGGTTCTTCCAG
CCCGACGCATACGCCGCCTTTGAGCGACAGGGCGAAAATCGTCAGCCTGCACTTGGAATGTTCGCCCTGTTTTAAGCGGG
AATGTCCGCTGGGGCATACCGACTGCCTCAACAGGCTGTATCCCGAGAAGATTGTGCAGGCGGTTGAAGAGGCGGTATGA

Upstream 100 bases:

>100_bases
CCAACGGTTTCGCGGTAAAATTCTGCCTGTCTGATTTCGGTTTCTTGTTATGTCCATCAAAATCCTGATTATTTCTCCCA
GTTGGATAGGCGACTGCGTG

Downstream 100 bases:

>100_bases
TGGGTTCTGTTTTCGGGTTTATGCCGTCTGACGGCGGATATTGATAGGATGTTTATGGCTATTGCGAACAATAAAAAAGC
ATTTCACGATTTTTTCATTG

Product: ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAI
IALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGK
PVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGSQKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADT
VVCNDSGLMHLAAALGRKVVAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEEAV

Sequences:

>Translated_319_residues
MTQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAI
IALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGK
PVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGSQKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADT
VVCNDSGLMHLAAALGRKVVAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEEAV
>Mature_318_residues
TQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAII
ALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGKP
VLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGSQKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTV
VCNDSGLMHLAAALGRKVVAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEEAV

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=322, Percent_Identity=44.0993788819876, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 35638; Mature: 35507

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENPFGHGALELKRRWRVGRD
CCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHH
LGRRGYDQVIVLPGSLKSAIIALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRY
HCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
TALAHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTR
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHEECCCCCCCHHEECCCCCCCCCCCCHHH
YFAELGKHYSAAGWQVWLFGSQKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADT
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
VVCNDSGLMHLAAALGRKVVAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHT
EEECCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHCCCCCCHH
DCLNRLYPEKIVQAVEEAV
HHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure 
TQPLFRRLKELHPGCTIDVFAPKWSMAVFERMPEVNEILENPFGHGALELKRRWRVGRD
CCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHH
LGRRGYDQVIVLPGSLKSAIIALATGIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRY
HCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
TALAHPSQEDFDGHSGFPEFSIDERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTR
HHHCCCCCCCCCCCCCCCCCCHHHHHHHCCHHEECCCCCCCHHEECCCCCCCCCCCCHHH
YFAELGKHYSAAGWQVWLFGSQKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADT
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
VVCNDSGLMHLAAALGRKVVAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHT
EEECCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCHHHHCCCCCCHH
DCLNRLYPEKIVQAVEEAV
HHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]