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The map label for this gene is lpdA2 [H]

Identifier: 161869859

GI number: 161869859

Start: 891231

End: 892583

Strand: Direct

Name: lpdA2 [H]

Synonym: NMCC_0890

Alternate gene names: 161869859

Gene position: 891231-892583 (Clockwise)

Preceding gene: 161869858

Following gene: 161869860

Centisome position: 41.39

GC content: 54.62

Gene sequence:

>1353_bases
ATGGGCGCGTTTCGCAATGCCCGTTTGCATTCGAACAATGTTTACCTGATTGAAAACAATGTGTTCGGCACGACCTGCGC
GCGGGTGGGTTGTATGCCTTCCAAACTCTTGATTGCCGCCGCAGAAGCGCGCCACCACGCACTGCATACCGACCCGTTCG
GCGTGCATTTGGACAAAGACAGCATCGTCGTCAACGGTGAAGAGGTCATGCAGCGCGTTAAATCCGAGCGTGACCGTTTT
GTCGGCTTTGTCGTTGCCGATGTGGAAGAGTGGCCTGCCGACAAGCGCATTATGGGTTCGGCTAAATTTATCGACGAGCA
TACCGTCCAAATCGACGAGCATACTCAAATTACGGCAAAAAGTTTCGTGATTGCTACCGGTTCGCGTCCCGTCATCCTGC
CGCAATGGCAGTCTTTGGGCAATCGTTTGATTATCAACGATGACGTTTTCTCATGGGATACGCTGCCTAAGCGCGTTGCC
GTGTTCGGGCCGGGTGTTATCGGTTTGGAACTGGGTCAGGCATTGCACCGTTTGGGCGTGAAAGTTGAAATTTTCGGTTT
GGGCGGAATCATCGGCGGCATTTCCGACCCCGTCGTTTCAGACGAGGCGAACGCCGTGTTCGGCGAAGAATTGAAACTGC
ATCTGGATGCTAAAACCGAGGTCAAACTCGATGCAGACGGCAATGTAGAAGTCCATTGGGAGCAGGATGGCGAAAAAGGC
GTATTTGTTGCCGAATATATGCTGGCAGCCGTGGGCCGCCGTCCGAACGTTGACAATATCGGTTTGGAAAATATCAATAT
CGAAAAAGATGCGCGCGGCGTACCTGTTGCCGACCCGCTGACCATGCAGACCAGTATTCCGCATATCTTCATCGCAGGCG
ATGCGTCCAACCAACTGCCTCTGCTGCATGAAGCTGCCGACCAAGGCAAGATTGCCGGCGATAACGCGGGCCGCTACCCG
AATATCGGCGGCGGTTTGCGGCGCAGCACCATCGGCGTGGTGTTTACCAGTCCGCAAATCGGCTTTGTCGGTCTGAAATA
CGCGCAGGTTGCCGCGCAATACCAAGCCGACGAATTTGTCATCGGCGAAGTATCGTTCAAAAACCAAGGCCGCAGCCGCG
TGATGCTGGTGAACAAAGGCCATATGCGCCTGTATGCCGAAAAAGCCACCGGCCGCTTTATCGGCGCGGAAATCGTAGGC
CCTGCCGCCGAACATTTGGCGCACCTGTTGGCTTGGGCACATCAAATGAAGATGACCGTTCCGCAAATGCTGGATATGCC
GTTCTACCATCCCGTTATCGAGGAAGGTCTGCGTACCGCGTTGCGCGATGCCGATGCGAAATTGAAAGCCTGA

Upstream 100 bases:

>100_bases
GCATCACGCTGATGCTGCTTCGGACGCTTAATAAAGAAGGACAAAGATTATGAAAAAAATTCAAGCAGATGTCGTCGTAA
TCGGCGGCGGTACTGCCGGT

Downstream 100 bases:

>100_bases
CCGATATGGCAAAACAATGCCGTCTGAAATTTTTTCAGACGGCATTTTGTTTTTGGGGATGGGGTCGGATGCTGATACCG
TGTCGGGAAGGGGGCGGCAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGAFRNARLHSNNVYLIENNVFGTTCARVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRF
VGFVVADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRLIINDDVFSWDTLPKRVA
VFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSDEANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKG
VFVAEYMLAAVGRRPNVDNIGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAGRYP
NIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVMLVNKGHMRLYAEKATGRFIGAEIVG
PAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVIEEGLRTALRDADAKLKA

Sequences:

>Translated_450_residues
MGAFRNARLHSNNVYLIENNVFGTTCARVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRF
VGFVVADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRLIINDDVFSWDTLPKRVA
VFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSDEANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKG
VFVAEYMLAAVGRRPNVDNIGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAGRYP
NIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVMLVNKGHMRLYAEKATGRFIGAEIVG
PAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVIEEGLRTALRDADAKLKA
>Mature_449_residues
GAFRNARLHSNNVYLIENNVFGTTCARVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFV
GFVVADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRLIINDDVFSWDTLPKRVAV
FGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSDEANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGV
FVAEYMLAAVGRRPNVDNIGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAGRYPN
IGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVMLVNKGHMRLYAEKATGRFIGAEIVGP
AAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVIEEGLRTALRDADAKLKA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=453, Percent_Identity=27.3730684326711, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI50301238, Length=440, Percent_Identity=23.8636363636364, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=24.1830065359477, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=24.1830065359477, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=24.1830065359477, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=23.5930735930736, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI148277065, Length=464, Percent_Identity=23.7068965517241, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI22035672, Length=447, Percent_Identity=23.0425055928412, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=26.6075388026608, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87082354, Length=437, Percent_Identity=26.0869565217391, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789915, Length=450, Percent_Identity=27.3333333333333, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87081717, Length=439, Percent_Identity=23.6902050113895, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=27.9475982532751, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=25.9507829977629, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=25.9507829977629, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=469, Percent_Identity=22.3880597014925, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17557007, Length=439, Percent_Identity=23.6902050113895, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=467, Percent_Identity=25.9100642398287, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325166, Length=452, Percent_Identity=22.5663716814159, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=370, Percent_Identity=23.7837837837838, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21358499, Length=451, Percent_Identity=28.6031042128603, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640553, Length=441, Percent_Identity=27.2108843537415, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24640549, Length=441, Percent_Identity=27.2108843537415, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24640551, Length=434, Percent_Identity=26.9585253456221, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17737741, Length=442, Percent_Identity=25.5656108597285, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49135; Mature: 49004

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAFRNARLHSNNVYLIENNVFGTTCARVGCMPSKLLIAAAEARHHALHTDPFGVHLDKD
CCCCCCCEEECCCEEEEECCCCCCHHHHHCCCCHHHEEEEHHHHHCCCCCCCCEEEECCC
SIVVNGEEVMQRVKSERDRFVGFVVADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAK
CEEECHHHHHHHHHHHHHHEEEEEEEEHHHCCCCCEECCCHHCCCCEEEEECCCCEEEEE
SFVIATGSRPVILPQWQSLGNRLIINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGV
EEEEECCCCCEEEECHHHHCCEEEEECCCCCCCCCCCEEEEECCCEEHHHHHHHHHHCCC
KVEIFGLGGIIGGISDPVVSDEANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKG
EEEEEEECCEECCCCCCCCCCCCCEEECCEEEEEECCCEEEEECCCCCEEEEECCCCCCC
VFVAEYMLAAVGRRPNVDNIGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLP
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCC
LLHEAADQGKIAGDNAGRYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFV
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEEE
IGEVSFKNQGRSRVMLVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTV
EEEEEECCCCCCEEEEEECCCEEEEEECCCCCEECHHHCCHHHHHHHHHHHHHHHHHCCH
PQMLDMPFYHPVIEEGLRTALRDADAKLKA
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GAFRNARLHSNNVYLIENNVFGTTCARVGCMPSKLLIAAAEARHHALHTDPFGVHLDKD
CCCCCCEEECCCEEEEECCCCCCHHHHHCCCCHHHEEEEHHHHHCCCCCCCCEEEECCC
SIVVNGEEVMQRVKSERDRFVGFVVADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAK
CEEECHHHHHHHHHHHHHHEEEEEEEEHHHCCCCCEECCCHHCCCCEEEEECCCCEEEEE
SFVIATGSRPVILPQWQSLGNRLIINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGV
EEEEECCCCCEEEECHHHHCCEEEEECCCCCCCCCCCEEEEECCCEEHHHHHHHHHHCCC
KVEIFGLGGIIGGISDPVVSDEANAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKG
EEEEEEECCEECCCCCCCCCCCCCEEECCEEEEEECCCEEEEECCCCCEEEEECCCCCCC
VFVAEYMLAAVGRRPNVDNIGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLP
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCC
LLHEAADQGKIAGDNAGRYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFV
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCEEE
IGEVSFKNQGRSRVMLVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTV
EEEEEECCCCCCEEEEEECCCEEEEEECCCCCEECHHHCCHHHHHHHHHHHHHHHHHCCH
PQMLDMPFYHPVIEEGLRTALRDADAKLKA
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]