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The map label for this gene is holB [H]

Identifier: 161869713

GI number: 161869713

Start: 738794

End: 739771

Strand: Direct

Name: holB [H]

Synonym: NMCC_0733

Alternate gene names: 161869713

Gene position: 738794-739771 (Clockwise)

Preceding gene: 161869712

Following gene: 161869714

Centisome position: 34.31

GC content: 51.64

Gene sequence:

>978_bases
ATGATTTATCCGTGGCATAATGAGCAATGGCGGCAGATTGCGGAACATTGGGAGCGTCGTCCCAATGCATGGCTGTTTGC
CGGCAAAAAAGATACGGGGAAAACTACATTTGCCCGCTTTGCGGCGAAGGCACTGTTGTGCGAAACCCCTGCACCGGGCT
GCAAACCCTGTGGCGAATGTATGTCCTGCCATCTGTTTGGACAGGGAAGCCATCCCGATTTTTACGAAATCACCCCCTTG
TCGGACGAACCCGAAAACGGACGCAAACTGTTGCAGATCAAAATCGATGCCGTCAGGGAAATCATCGATAATGTGTACCT
GACTTCGGTACGGGGCGGTTTGCGCGTGGTTCTGATTCATCCTGCGGAAAGTATGAATATCCAGGCCGCCAACAGTTTGT
TGAAAGTGTTGGAAGAGCCTCCGCCACAAGTGGTCTTTTTGCTGGTCAGCCACGCGGCGGACAAGGTTTTACCGACCATT
AAAAGCCGCTGCCGGAAGATGGTTTTGCCCGCGCCTTCCCATGAAGAGGCATTGGCATATCTGCGTGAAAGGGGTGTGGC
GGAACCTGAGGAACGTCTGGCTTTCCATTCCGGAGCGCCGCTGTTTGATGAGGCGGACGGTGTCCGTGCGTTGCGGATTA
AACTGTTGGATATTTTGGCAGAACCGAGGTTGTTGAAGATTTTGGATTACGCCGCGCTTTTCGATAAGGAAAAACTTCCG
CTCGCCGTATTTGTCGGGTGGATGCAGAAATGGCTGGTCGATTTGGGATTGTGCCTGCAACACATGAAACCCGTCTATTA
TCCCGCTTATGAAGACAGGCTGCTTCAGACGGCATCCGGTTTCCGCCTGCGCAATGTATTTGCGGCGGAGGATATGCTCA
AACAGCTTGCCCCCTACGGGTTTCATACTTTAAATGTCAAAATGCAGATCGAGCATCTGCTCATCAACTATTTGGAATTG
AAGAAAGAGAACGGGTGA

Upstream 100 bases:

>100_bases
GCGTTTGGCGGTGCAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGGAACTGCTCTGATGGCGGTATGGAT
TTCCGGACGGATGGTTTGAA

Downstream 100 bases:

>100_bases
ATTATGTCAGACGGACAAAATATTCCGGCAAAAATGATGTCGTTGCAGCTGAAAGACATGAATCTGCTGTACAGCTCCTA
CATGCCGTTTTTGGAACACG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKALLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPL
SDEPENGRKLLQIKIDAVREIIDNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDILAEPRLLKILDYAALFDKEKLP
LAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQTASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLEL
KKENG

Sequences:

>Translated_325_residues
MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKALLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPL
SDEPENGRKLLQIKIDAVREIIDNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDILAEPRLLKILDYAALFDKEKLP
LAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQTASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLEL
KKENG
>Mature_325_residues
MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKALLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPL
SDEPENGRKLLQIKIDAVREIIDNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDILAEPRLLKILDYAALFDKEKLP
LAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQTASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLEL
KKENG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=150, Percent_Identity=38, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=33.1491712707182, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36990; Mature: 36990

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKALLCETPAPGCKPCGEC
CCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
MSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREIIDNVYLTSVRGGLRVVLIH
HEEEEECCCCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHEECCCEEEEEEC
PAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTIKSRCRKMVLPAPSHEEALAY
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDILAEPRLLKILDYAALFDKEKLP
HHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQTASGFRLRNVFAAEDMLKQLAPYG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
FHTLNVKMQIEHLLINYLELKKENG
EEEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKALLCETPAPGCKPCGEC
CCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
MSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREIIDNVYLTSVRGGLRVVLIH
HEEEEECCCCCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHEECCCEEEEEEC
PAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTIKSRCRKMVLPAPSHEEALAY
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDILAEPRLLKILDYAALFDKEKLP
HHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
LAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQTASGFRLRNVFAAEDMLKQLAPYG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
FHTLNVKMQIEHLLINYLELKKENG
EEEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]