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The map label for this gene is pilT2 [H]

Identifier: 161869712

GI number: 161869712

Start: 737630

End: 738760

Strand: Direct

Name: pilT2 [H]

Synonym: NMCC_0732

Alternate gene names: 161869712

Gene position: 737630-738760 (Clockwise)

Preceding gene: 161869708

Following gene: 161869713

Centisome position: 34.25

GC content: 54.02

Gene sequence:

>1131_bases
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACCCATTTCCCGCCCGCGATGAAGTTGGACGGCAAAATCACCCGCATCACGGACGAGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCTTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTGCAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Upstream 100 bases:

>100_bases
GGAGGTGGTTTGTCCGTGCCGTCTGAAACGGTTTCAGACGGCACGGCGGGTTTTTGGTAGAATGGGAAGGTACAGATTGT
TTGAAGATTAGGGGACGAGG

Downstream 100 bases:

>100_bases
TGGCGGTATGGATTTCCGGACGGATGGTTTGAAATGATTTATCCGTGGCATAATGAGCAATGGCGGCAGATTGCGGAACA
TTGGGAGCGTCGTCCCAATG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Sequences:

>Translated_376_residues
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSSPDLELL
>Mature_376_residues
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=308, Percent_Identity=36.038961038961, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786296, Length=371, Percent_Identity=26.1455525606469, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789723, Length=148, Percent_Identity=39.1891891891892, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42216; Mature: 42216

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCEEEEECCCCCCHHHHHHH
AFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLN
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
LPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHIITIEDPIEFVHEHKN
CCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCC
CIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
EEEEEHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEECCCCEEEEEEEC
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLI
CCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHH
SELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHH
LRSRRAQSSSPDLELL
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCEEEEECCCCCCHHHHHHH
AFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLN
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
LPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHIITIEDPIEFVHEHKN
CCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCC
CIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
EEEEEHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEECCCCEEEEEEEC
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLI
CCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHH
SELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHH
LRSRRAQSSSPDLELL
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]