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The map label for this gene is bioA [H]

Identifier: 161869674

GI number: 161869674

Start: 703560

End: 705011

Strand: Direct

Name: bioA [H]

Synonym: NMCC_0692

Alternate gene names: 161869674

Gene position: 703560-705011 (Clockwise)

Preceding gene: 161869673

Following gene: 161869675

Centisome position: 32.67

GC content: 53.44

Gene sequence:

>1452_bases
TTGGGGAATGCGAAACGGTGTGCGCTTATACTGCGGACGATTTGTTTCGCGGTTTTGCGCCCGAAACGGATGGAGAGGTG
TGGGAAACGGTCTGTCGGAGTAGAATACGCGTTTGCGTTTGAATACAGTAAGAAGAAAAGAGAGAAACTTATGCCGTCTG
AACATCAACACATATCATCATTGCTCGATTTTGACCGTACCCATCTGCTTCATCCCTATACTTCCATGACCGATCCGCTG
CCCGTTTATCCTGTCAAACGTGCAGAAGGGGTGTTTATCGAATTGGCGGACGGCACGCGGTTGATTGACGGGATGTCCTC
CTGGTGGTGTGCGATACACGGCTACAATCATCCTGTTTTGAATCAGGCGGTTGAGACGCAGATGAAACAGATGTCGCATG
TGATGTTCGGCGGTTTGACGCATGAGCCGGCGGTGGAGCTAGGCAAATTATTGGTCGGGATTTTGCCGCAGGGGCTGGAC
CGTATTTTTTATGCGGATTCGGGTTCGGTTTCGGTGGAAGTCGCGTTGAAGATGGTGGTGCAATATCAGCAGGCGCGGGG
TTTGCGTGCAAAACAAAATATGGCGACGGTACGGCGCGGGTATCACGGCGATACTTGGAACGCGATGTCCGTCTGCGATC
CGGAAACGGGGATGCACCATATTTTCGGCAGCGCGTTGCCGCAGCGTTATTTTGTCGATAATCCGAAAAGCCGTTTCGAC
GATGAATGGGACGGGGCGGATTTGCAGCCTGTCCGCGCCTTGTTTGAAGTGCATCATGCGGATATTGCCGCCTTTATTTT
AGAGCCGGTCGTGCAGGGCGCGGGCGGCATGTATTTTTATCATCCGCAGTATCTTCGCGGATTGCGCGATTTGTGCGACG
AGTTTGATATCGTTTTGATTTTTGATGAAATCGCCACCGGATTCGGGCGCACGGGCAAGATGTTTGCCTGCGAACACGCG
GAGGTCGTGCCGGATATTATGTGCATCGGCAAGGGTTTGAGCGGCGGCTATATGACGCTGGCGGCAGCAATCACTTCGCA
AAAAGTTACCGAAACGATTTCGCGCGGCGAAGCGGGCGTGTTTATGCACGGCCCGACGTTTATGGCAAACCCGCTGGCGT
GTGCCGTTGCCTGCGCTTCGGTCAAACTGCTTTTGTCTCAAGACTGGCAGGCAAATATCCGCCGCATTGAAAGCATCTTA
AAAGGCCGTCTGAAAGCCGCGTGGGACATTCGCGGCGTGAAAGACGTACGCGTTTTGGGTGCCATCGGCGTGATCGAGCT
GGATAAAGGCGTGGATATGGCGCGTTTTCAAGCGGATTGCGTGGCGCAGGGCATTTGGGTGCGCCCGTTCGGCAGGCTGG
TGTATCTGATGCCGCCTTACATCATTTCAGACGGCGTTTTGACCAAACTTGCCGACAAAACCGTGCAAATCTTGAAGGAA
CACAGCAAATGA

Upstream 100 bases:

>100_bases
GTATCGTATGGATTGCCGTTTTGTCTATGACTGATTGAAGCGTTGGGCGGAGGCTGTGTGAAACAATATTGGGCGTTGGG
TCGTCTGATTCCAATCGGGC

Downstream 100 bases:

>100_bases
AAGGCGTTTACTTCGTCAGCGGCATAGACACGGACATCGGCAAAACCGTCGCCACCGGCGTGTTGGCAAAACAATTGTTG
CAGCAGGGCAAAAGCGTGAT

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGNAKRCALILRTICFAVLRPKRMERCGKRSVGVEYAFAFEYSKKKREKLMPSEHQHISSLLDFDRTHLLHPYTSMTDPL
PVYPVKRAEGVFIELADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLVGILPQGLD
RIFYADSGSVSVEVALKMVVQYQQARGLRAKQNMATVRRGYHGDTWNAMSVCDPETGMHHIFGSALPQRYFVDNPKSRFD
DEWDGADLQPVRALFEVHHADIAAFILEPVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHA
EVVPDIMCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLLLSQDWQANIRRIESIL
KGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVAQGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKE
HSK

Sequences:

>Translated_483_residues
MGNAKRCALILRTICFAVLRPKRMERCGKRSVGVEYAFAFEYSKKKREKLMPSEHQHISSLLDFDRTHLLHPYTSMTDPL
PVYPVKRAEGVFIELADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLVGILPQGLD
RIFYADSGSVSVEVALKMVVQYQQARGLRAKQNMATVRRGYHGDTWNAMSVCDPETGMHHIFGSALPQRYFVDNPKSRFD
DEWDGADLQPVRALFEVHHADIAAFILEPVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHA
EVVPDIMCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLLLSQDWQANIRRIESIL
KGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVAQGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKE
HSK
>Mature_482_residues
GNAKRCALILRTICFAVLRPKRMERCGKRSVGVEYAFAFEYSKKKREKLMPSEHQHISSLLDFDRTHLLHPYTSMTDPLP
VYPVKRAEGVFIELADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLVGILPQGLDR
IFYADSGSVSVEVALKMVVQYQQARGLRAKQNMATVRRGYHGDTWNAMSVCDPETGMHHIFGSALPQRYFVDNPKSRFDD
EWDGADLQPVRALFEVHHADIAAFILEPVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAE
VVPDIMCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLLLSQDWQANIRRIESILK
GRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVAQGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEH
SK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=413, Percent_Identity=27.3607748184019, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI284507298, Length=241, Percent_Identity=32.7800829875519, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI13994255, Length=459, Percent_Identity=25.0544662309368, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI37574042, Length=429, Percent_Identity=25.1748251748252, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI226442709, Length=392, Percent_Identity=25.765306122449, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI226442705, Length=359, Percent_Identity=25.9052924791086, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI24119277, Length=381, Percent_Identity=25.9842519685039, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786991, Length=421, Percent_Identity=57.4821852731591, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI145693181, Length=306, Percent_Identity=35.6209150326797, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789759, Length=395, Percent_Identity=28.8607594936709, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789016, Length=400, Percent_Identity=28.75, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788044, Length=399, Percent_Identity=28.0701754385965, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787560, Length=409, Percent_Identity=26.6503667481663, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786349, Length=337, Percent_Identity=27.893175074184, Blast_Score=105, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=445, Percent_Identity=26.9662921348315, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25144274, Length=229, Percent_Identity=31.8777292576419, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32564660, Length=443, Percent_Identity=23.7020316027088, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71992977, Length=435, Percent_Identity=20.4597701149425, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324386, Length=465, Percent_Identity=43.010752688172, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323470, Length=399, Percent_Identity=27.0676691729323, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=316, Percent_Identity=26.8987341772152, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321456, Length=315, Percent_Identity=25.0793650793651, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357415, Length=395, Percent_Identity=27.3417721518987, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI21356575, Length=368, Percent_Identity=27.1739130434783, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574759, Length=435, Percent_Identity=24.1379310344828, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI161085790, Length=435, Percent_Identity=24.1379310344828, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281366494, Length=291, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24667139, Length=291, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24667143, Length=291, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 53867; Mature: 53736

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNAKRCALILRTICFAVLRPKRMERCGKRSVGVEYAFAFEYSKKKREKLMPSEHQHISS
CCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHH
LLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIELADGTRLIDGMSSWWCAIHGYNHPVL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCCCHHH
NQAVETQMKQMSHVMFGGLTHEPAVELGKLLVGILPQGLDRIFYADSGSVSVEVALKMVV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
QYQQARGLRAKQNMATVRRGYHGDTWNAMSVCDPETGMHHIFGSALPQRYFVDNPKSRFD
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCC
DEWDGADLQPVRALFEVHHADIAAFILEPVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCEEEE
FDEIATGFGRTGKMFACEHAEVVPDIMCIGKGLSGGYMTLAAAITSQKVTETISRGEAGV
EHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
FMHGPTFMANPLACAVACASVKLLLSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLG
EEECCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AIGVIELDKGVDMARFQADCVAQGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKE
CCCEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHH
HSK
CCC
>Mature Secondary Structure 
GNAKRCALILRTICFAVLRPKRMERCGKRSVGVEYAFAFEYSKKKREKLMPSEHQHISS
CCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHH
LLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIELADGTRLIDGMSSWWCAIHGYNHPVL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCCCHHH
NQAVETQMKQMSHVMFGGLTHEPAVELGKLLVGILPQGLDRIFYADSGSVSVEVALKMVV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
QYQQARGLRAKQNMATVRRGYHGDTWNAMSVCDPETGMHHIFGSALPQRYFVDNPKSRFD
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHCC
DEWDGADLQPVRALFEVHHADIAAFILEPVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCEEEE
FDEIATGFGRTGKMFACEHAEVVPDIMCIGKGLSGGYMTLAAAITSQKVTETISRGEAGV
EHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
FMHGPTFMANPLACAVACASVKLLLSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLG
EEECCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AIGVIELDKGVDMARFQADCVAQGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKE
CCCEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHH
HSK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]