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The map label for this gene is bioD [H]

Identifier: 161869675

GI number: 161869675

Start: 705008

End: 705655

Strand: Direct

Name: bioD [H]

Synonym: NMCC_0693

Alternate gene names: 161869675

Gene position: 705008-705655 (Clockwise)

Preceding gene: 161869674

Following gene: 161869676

Centisome position: 32.74

GC content: 53.7

Gene sequence:

>648_bases
ATGAAAGGCGTTTACTTCGTCAGCGGCATAGACACGGACATCGGCAAAACCGTCGCCACCGGCGTGTTGGCAAAACAATT
GTTGCAGCAGGGCAAAAGCGTGATTACGCAAAAGCCCGTGCAAACCGGTTGCCAAAACATTGCCGACGACATCGCCGTCC
ATCGTAAAATCATGGGTAAACCCATGCAGGAAGCCGACAAACAAGGGCTGACCATGCCTGAAATCTTCAGTTATCCAGCT
TCGCCTCATCTCGCTGCCCGTCTTGACGGCAGGGCTTTGGACTTGGACAAAATCCGCACCGCCACGCAGCAACTGGCGGC
GCAATACGAAATCGTCTTGGTCGAAGGCGCGGGCGGATTGATGGTTCCGCTGACGGAAAACCTGTTAACCATTGATTATA
TCCGTCAGCAAGGCTATCCCGTCATCCTCGTTACCAGCGGACGGCTGGGCAGTATCAACCACACTTTACTCAGTTTCGCC
GCGCTCAAACAATACGGCATCCGTCTGCACAGCCTCGTGTTCAACCACATCCACGACAGCCGCGACGCACACATCGCCCA
AGACAGCCTGAACTACTTGAAATGCCGTCTGAAAGCCGATTTTTCCGAAGCGGAATGGATGGAGTTGGCAAAAACAGACG
CGGTATAA

Upstream 100 bases:

>100_bases
TCGGCAGGCTGGTGTATCTGATGCCGCCTTACATCATTTCAGACGGCGTTTTGACCAAACTTGCCGACAAAACCGTGCAA
ATCTTGAAGGAACACAGCAA

Downstream 100 bases:

>100_bases
AGATTGGGAAAAATATGGAACACCTATTTGGGAAATGGCTGCCCGACTTGCCCACCCCCATTTCAGACGGCATCAGCCTG
CCGATGGTGCGGCTGCTGCA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGCQNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPA
SPHLAARLDGRALDLDKIRTATQQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV

Sequences:

>Translated_215_residues
MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGCQNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPA
SPHLAARLDGRALDLDKIRTATQQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV
>Mature_215_residues
MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGCQNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPA
SPHLAARLDGRALDLDKIRTATQQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=188, Percent_Identity=31.3829787234043, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI87081944, Length=183, Percent_Identity=26.2295081967213, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324385, Length=186, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23630; Mature: 23630

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGCQNIADDIAVHRKIMGK
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
PMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTATQQLAAQYEIVLVEGAGGL
CHHHHHHCCCCCHHHHCCCCCCCCEEECCCCEECHHHHHHHHHHHHHCEEEEEEECCCCE
MVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFAALKQYGIRLHSLVFNHIHDS
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV
CCCHHHHHHHHHHEEHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGCQNIADDIAVHRKIMGK
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
PMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTATQQLAAQYEIVLVEGAGGL
CHHHHHHCCCCCHHHHCCCCCCCCEEECCCCEECHHHHHHHHHHHHHCEEEEEEECCCCE
MVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFAALKQYGIRLHSLVFNHIHDS
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV
CCCHHHHHHHHHHEEHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]