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The map label for this gene is rpoH [H]

Identifier: 161869654

GI number: 161869654

Start: 684796

End: 685659

Strand: Reverse

Name: rpoH [H]

Synonym: NMCC_0671

Alternate gene names: 161869654

Gene position: 685659-684796 (Counterclockwise)

Preceding gene: 161869655

Following gene: 161869653

Centisome position: 31.84

GC content: 54.86

Gene sequence:

>864_bases
ATGAATAACGCTTTCGCATTACCCGCCATCCAAAGCGGCAACGGCAGCCTCGAACAATACATCCACACCGTCAACAGAAT
TCCCATGCTGACCCAAGAGGAAGAAACACGCCTTGCCGAACGCCAACACAAAGGCGACCTCAACGCCGCCAAACAACTCA
TCCTGTCCCACCTGCGCGTCGTCGTTTCCATCGCGCGCGGCTATGACGGCTACGGGCTGAATCAGGCAGACCTGATTCAG
GAAGGCAATATCGGACTGATGAAGGCGGTCAAACGCTACGAGCCCGGTAGAGGCGCGCGTCTGTTTTCATTTGCCGTACA
CTGGATTAAAGCCGAAATCCACGAGTTTATTTTGAGAAACTGGCGTTTGGTTCGCGTTGCCACCACCAAACCGCAACGCA
AGCTGTTTTTCAACCTGCGCAGCATGCGTAAAAACCTCAACGCCCTGTCTCCCAAAGAGGCACAAGACATCGCCGACGAT
TTGGGTGTCAAACTTTCCGAAGTTCTGGAAATGGAACAGCGAATGACGGGACACGACATCGCCATCATGGCAGACAACAG
CGACGACGAGGATAGTTTCGCCCCCATCGACTGGCTTGCCGACCACGATTCCGAACCGAGCCGACAACTATCGAAACAGG
CGCATTACGCCCTGCAAACAGAAGGTTTACAAAACGCTTTGGCGCAACTGGATGACAGGAGCCGCCGTATTGTGGAAAGC
CGCTGGCTTCAAGACGACGGCGGGCTGACGCTGCACCAGCTTGCCGCCGAATACGGCGTATCTGCCGAACGCATCCGCCA
AATCGAAGCAAAAGCCATGCAGAAACTGCGTGGTTTCCTGACCGAAGAAGCCGAAGCGGTTTGA

Upstream 100 bases:

>100_bases
CTCCTGCCAAATATACAAAATACAAGCCAAGCAGTGATATACTGCATCCCAACACAGCAAACCGCCGATACGCAGTTTCA
GCCGAAAGGAAATGTCCCAA

Downstream 100 bases:

>100_bases
AATATACGCAATAAAATACCCGACAAATTGTTTTGTCGGGTATTTTATTTGGTAAACATCAATGCGGTTTGTGCGTCGTG
CTGTACACGATTTTATCCGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHKGDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQ
EGNIGLMKAVKRYEPGRGARLFSFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYALQTEGLQNALAQLDDRSRRIVES
RWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKLRGFLTEEAEAV

Sequences:

>Translated_287_residues
MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHKGDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQ
EGNIGLMKAVKRYEPGRGARLFSFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYALQTEGLQNALAQLDDRSRRIVES
RWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKLRGFLTEEAEAV
>Mature_287_residues
MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHKGDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQ
EGNIGLMKAVKRYEPGRGARLFSFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYALQTEGLQNALAQLDDRSRRIVES
RWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKLRGFLTEEAEAV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=267, Percent_Identity=52.8089887640449, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1789098, Length=266, Percent_Identity=30.4511278195489, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=32.4723247232472, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32524; Mature: 32524

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHKGDLNAAKQLILSHLRV
CCCCEECCCEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLFSFAVHWIKAEIHEFILRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADDLGVKLSEVLEMEQRMTGHDI
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
AIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYALQTEGLQNALAQLDDRSRRIVES
EEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKLRGFLTEEAEAV
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHKGDLNAAKQLILSHLRV
CCCCEECCCEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLFSFAVHWIKAEIHEFILRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADDLGVKLSEVLEMEQRMTGHDI
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
AIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYALQTEGLQNALAQLDDRSRRIVES
EEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKLRGFLTEEAEAV
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301 [H]