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The map label for this gene is greB [H]

Identifier: 161869630

GI number: 161869630

Start: 656838

End: 657329

Strand: Reverse

Name: greB [H]

Synonym: NMCC_0647

Alternate gene names: 161869630

Gene position: 657329-656838 (Counterclockwise)

Preceding gene: 161869631

Following gene: 161869629

Centisome position: 30.52

GC content: 54.67

Gene sequence:

>492_bases
ATGAGTACCGAAACCAAAAACTACATTACGCCCGCCGGATGGCAGGCATTGAAAGACGAGCTTTACCGGCTGGTCAACAA
AGAACGCCCCGAAATCGTCCAAATCGTCAACTGGGCGGCAGGCAACGGCGACCGCAGCGAAAACGGCGACTATCTTTACG
GCAAACGCCGTATGCGCGAAATCGACCGCCGCATCCGTTTCCTGACCAAACGTTTGGAGTCTGCCGTCGTCGTCGATCCC
GAATTGCGCGAAGCGACCGACCAAGTGTTTTTCGGCGCGACGGTCGGGCTGTTGCGCGGCGACGGGCGCGAGCAAACCGT
CAAAATTGTCGGCATCGATGAAATCGATACCGCGCAAAACAAAATTTCTTGGATTTCGCCGTTGGCGCGTTGCCTGATTA
AAGCGCGCGAAGGCGACGAAGTGGTTTTGAACACGCCGGAAGGGCGTGAGGAAATTGAAATCCTTTCTGTCGAATACATC
AAAATCGACTGA

Upstream 100 bases:

>100_bases
AGGCTTCCCAAACCGCGATGCCGTCTGAAATCCGAACGCCGGGTTTTCAGACGGCATTTCCCATATCGGCATCACATTTC
CCATTTCATCCGCACGCAAT

Downstream 100 bases:

>100_bases
TCGGGAATGCCGTCTGAAACGGCACAAACGCATCGTTTCCCAAATAAAGGAATACCGAATGAGGAAAATCCGCACCAAAA
TCTGCGGCATCACCACACTG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGDRSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDP
ELREATDQVFFGATVGLLRGDGREQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
KID

Sequences:

>Translated_163_residues
MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGDRSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDP
ELREATDQVFFGATVGLLRGDGREQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
KID
>Mature_162_residues
STETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGDRSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPE
LREATDQVFFGATVGLLRGDGREQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYIK
ID

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=47.0588235294118, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=39.8692810457516, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18652; Mature: 18521

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGDRSENGDYLYGKRRMRE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEHHHHHHHH
IDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDGREQTVKIVGIDEIDTAQN
HHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHEEEHCCCCCCCEEEEEECCCCCHHHH
KISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYIKID
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEEEC
>Mature Secondary Structure 
STETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGDRSENGDYLYGKRRMRE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEHHHHHHHH
IDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDGREQTVKIVGIDEIDTAQN
HHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHEEEHCCCCCCCEEEEEECCCCCHHHH
KISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYIKID
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]