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The map label for this gene is purF [H]

Identifier: 161869631

GI number: 161869631

Start: 657429

End: 658844

Strand: Reverse

Name: purF [H]

Synonym: NMCC_0648

Alternate gene names: 161869631

Gene position: 658844-657429 (Counterclockwise)

Preceding gene: 161869632

Following gene: 161869630

Centisome position: 30.6

GC content: 58.19

Gene sequence:

>1416_bases
ATGCACAAAGGCAAAGGCATGGTGCGCGAAGTGTTCCGCACACGCAATATGCGCGATTTGACCGGCAACGCCGGTATCGC
CCACGTCCGTTATCCTACTGCGGGCAACGCGGGCAGCAGCGCGGAGGCGCAGCCTTTCTACGTCAGCTCGCCGTTCGGCA
TCGTTTTGGCGCATAACGGCAACCTCACCAACACGGCCGAACTTTATGAAAACGTGTGCAACAAACACCTGCGCCACGTC
AACACCAGCTCCGATTCCGAAGTCTTGCTCAACGTATTCGCGCACGAATTGCGCCGCGAAGTCTCTAAAAACGCCGACCC
GCACCGGCTCAATGCCGACAATATTTTCAACGCCGTTGCCGAAGTCCACCGCCTGGTGCACGGCGCATACGGTGTGGTTG
CCATGATTGCGGGCTACGGTATGCTTGCCTTCCGCGACCCTTACGGCATCCGCCCGCTGGTATTGGGTTCGCAAACCGAC
AACGAAGGCAGGAAATCCTATGCAGTCGCCTCCGAATCCGTCGCCTTCAACGCGCTTGCCTACGATTTGGAACGCGACAT
CCGGCCGGGCGAAGCGGTATTTGTCGGCTTTGACGGCACAATGATTGCCCGCCAATGCAGCGACCGTGCCAAACTCAGCC
CCTGCCTTTTTGAATATGTTTATTTTGCCCGCCCCGACTCCGTAATCGACGGCGTATCCGTTTACCAGTCGCGCTTGGAT
ATGGGCGTGTCCTTGGCGGAAAAAATCAAACGCGAGCTGCCCGTGGACGGCATCGACGTTGTAATGCCCATCCCCGACAC
CAGCCGCCCCAGCGCGATGGAGCTTGCCGTCCACCTCGACAAACCCTACCGCGAGGGCTTGATTAAAAACCGCTATATCG
GCCGCACCTTTATTATGCCCGGCCAGGCGACGCGCAAAAAATCCGTGCGCCAAAAACTCAGCCCGATGGAAACCGAGTTT
GCAGGCAAAAGCGTGTTGCTGGTGGACGACTCCATCGTGCGCGGAACGACCAGCCGCGAAATCGTCGAAATGGTACGCGC
GGCGGGCGCGCGCAAAGTCTATATCGCCTCCGCCGCCCCCGAAGTGCGCTATCCCAACGTATACGGCATCGATATGCCCA
CGCGCGAAGAGTTGATTGCCAATGGGCGCAGCGCGGCGGAAATCGCCGCCGAAATCGGCGCAGACGGCATCGTGTTCCAA
AATTTGAGCGATTTGGAAGCCGTCGTCAAAGCACTCAACCCGAAAATCGAATCCTTCGATTCGTCTTGTTTCAACGGCAT
TTATCAGACCGGCGACATCGATGATGCCTACCTCGACCGCCTGTCTGCCGAGAAATCCGGCTGCGGCGGCCTGAAAATCC
ACCCGAGCAGGATGGAACACAGCATCAGCATCAGCGATGCGGGCGACGAAGAATAA

Upstream 100 bases:

>100_bases
TGAACCCGTGAACCAGCTTCTGTACGACGGCTTGCAGATGTTGCAGCACAGGGGGCAGGATGCGGCGGGCATTGCAACGG
CGGAAGGCGGTACCTTCCAT

Downstream 100 bases:

>100_bases
GGCTTCCCAAACCGCGATGCCGTCTGAAATCCGAACGCCGGGTTTTCAGACGGCATTTCCCATATCGGCATCACATTTCC
CATTTCATCCGCACGCAATA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MHKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHNGNLTNTAELYENVCNKHLRHV
NTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFNAVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTD
NEGRKSYAVASESVAFNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVSVYQSRLD
MGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMPGQATRKKSVRQKLSPMETEF
AGKSVLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQ
NLSDLEAVVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISISDAGDEE

Sequences:

>Translated_471_residues
MHKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHNGNLTNTAELYENVCNKHLRHV
NTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFNAVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTD
NEGRKSYAVASESVAFNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVSVYQSRLD
MGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMPGQATRKKSVRQKLSPMETEF
AGKSVLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQ
NLSDLEAVVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISISDAGDEE
>Mature_471_residues
MHKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHNGNLTNTAELYENVCNKHLRHV
NTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFNAVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTD
NEGRKSYAVASESVAFNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVSVYQSRLD
MGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMPGQATRKKSVRQKLSPMETEF
AGKSVLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQ
NLSDLEAVVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISISDAGDEE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=430, Percent_Identity=36.2790697674419, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1788651, Length=441, Percent_Identity=58.9569160997732, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17554892, Length=434, Percent_Identity=32.7188940092166, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323958, Length=446, Percent_Identity=50.8968609865471, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24659604, Length=447, Percent_Identity=35.1230425055928, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24659598, Length=447, Percent_Identity=35.1230425055928, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI28573187, Length=454, Percent_Identity=34.3612334801762, Blast_Score=233, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51584; Mature: 51584

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHNG
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECC
NLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFNAVA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVAFNALA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVSVYQSRLD
HHHHHCCCCCCEEEEECCCHHHHHHHCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
MGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMP
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECC
GQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAP
CCHHHHHHHHHHCCCCHHHHCCCEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEEECCC
EVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEAVVKALNPKIESFD
CCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCHHHHH
SSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISISDAGDEE
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECHHHHCCEEEECCCCCCC
>Mature Secondary Structure
MHKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHNG
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECC
NLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFNAVA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVAFNALA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVSVYQSRLD
HHHHHCCCCCCEEEEECCCHHHHHHHCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
MGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNRYIGRTFIMP
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEECC
GQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAGARKVYIASAAP
CCHHHHHHHHHHCCCCHHHHCCCEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEEECCC
EVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEAVVKALNPKIESFD
CCCCCCEEECCCCCHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCHHHHH
SSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISISDAGDEE
HHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]