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The map label for this gene is ligA

Identifier: 161869603

GI number: 161869603

Start: 634507

End: 637032

Strand: Reverse

Name: ligA

Synonym: NMCC_0619

Alternate gene names: 161869603

Gene position: 637032-634507 (Counterclockwise)

Preceding gene: 161869604

Following gene: 161869602

Centisome position: 29.58

GC content: 58.51

Gene sequence:

>2526_bases
ATGAATCCAAACTCAAAACATAACACTAACTTTACCAATCTTCTTAAACCGTCTGATAGTGATATAAAACAATTTGCCGC
ACAACACATCTGCCGCCTCACCGACCTCCTCAACCGCTACGCCTACGAATACTACACCCTCGACGCGCCCAGCGTACCCG
ATGCCGAATACGACAAATTGTTCCGCGAACTCGAAGCGTTGGAGTTAAACCATCCCGAGCTCAAACTGCCCGACAGCCCG
ACCCAGCGCGTCGGCGGCGAGCCTTTGGCGGGATTTGCCGAAGTACGCCACGAAGTGCCGATGCTGTCGCTGACCAACGC
TTTTTCCCCGCAAGATGAAAACGGCGTGTTCGACCATGCCGAAATGTACGCCTTCGACCAACGCGTGCGCGACGGCTTGG
ACGGCGGCAATCCCGAATACGTTATCGAACCCAAATTCGACGGCCTCGCCATCAGCCTGCTCTACCGCGACGGCGTATTG
GTGCAGGCGGCAACGCGCGGCGACGGCACGACGGGCGAAGACGTTACCCAAAACATCAAAACCGTATCCAACATCCCCTT
GCGGCTGCACGGCGAAAATACGCCCGAACTCATCGAGGTACGCGGCGAAGTGCTGATGCTCAAAGCCGATTTTGTCGCCC
TCAATAAAAGACAAGCCGAAAACGGGCAAAAACCCTTTGCCAATCCGCGCAACGCCGCCGCCGGCAGCCTACGCCAACTC
GATTCGCGCATCACCGCGCAACGCAAACTGCACTTTTTCCCCTACTCCGTCGCCCGCCAGCAAGATGGTTTCGTCGCGGA
AGAACACATCCAGGAACTCGCCTATTTCCAAGCACTCGGCTTCAGTCTGCCCAATGGCAATTTCGGCTGTTTCAAAAATA
TCGACGAAGTATTGGCGTTTTACGAACACATGCAGCAAAAACGCCCCGAACTGCCCTACGAAATCGACGGCATGGTCGTC
AAAGTCAACAGCTTGGCGCAACAGCACGAACTCGGCTTCATCTCCCGCGCGCCGCGTTGGGCGGTTGCCCACAAATTCCC
TGCCGAAGAAGCCCTGACCATTGTCGAAGCGATTGACGTGCAAATCGGCAGGACGGGCGCGGTAACGCCGGTTGCCCGAT
TACAGCCCGTATTCGTCGGTGGCGTAACTGTTACCAACGCCACCCTGCACAATCAGGACGAAGTATCGCGCAAAGACGTT
CGCGTCGGCGATACCGTCGTCGTACGCCGTGCCGGAGACGTGATTCCCGAAGTTGTGCGCGTGATTTTTGAACGCCGCCC
GATGCGGGAAACCGCCGTTGCCGTTTCAGACGGCATCGGGCATCGGCAGGACGATTTGTTTGCCGAAACACCGTCCGCCA
ATCAAACCCAATCCGTTCCGCTCCACAAGCCCTACCGCCTGCCGACCCACTGCCCCATCTGCCGCAGCGAAATCGAACGC
GAAGAAGGCGAAGCCGTCGCCCGATGCAGCGGCGGTATGCTTTGTCAGGCACAACGCGCGCAAGGCTTAATCCACTTCGC
CTCGCGCAAAGCGATGGACATCGACGGCTTGGGTGAAAAACAAATCGAACAGCTTGTCGCGCAAGACCTCGTCCGCCACT
TCGCCGATCTCTACCGCCTCGACATCCCGACCTTGCAAAAAATGAAGGAAACAGCGGACAAAACCGTTGCCGAATCGGAT
CAAATGCCGTCTGAAGGCAGTTCGGTCGGCGCGTCGGGCAAACATAAAAAACAACCAGTCAAGTGGGCGGAAAACATCCT
CGCAGGCATAGAAGCCAGCAAAACGCCCGAACTCGCCCGCTTCCTGTTCGCGCTCGGCATCCGCCACGTCGGCGAACGCA
CCGCCAAAACGCTGGCACAGGCATTCGGCACATTGGAACGCGTCCGCCGCGCCCCCGAACCCGTCCTCGCCTGCCTGCCC
GACATCGGTACGGTAGTGGCGCGTTCCATTGCCCACTTCTTCGCCCAAGCCGAACAGCAGGCGATGATAGACGAACTGCT
CGCCGCAGGCGTTGCCCCGCAAACCCAAGCCGTTACCATCCCGCCCGCCCGCCACGCCGAACCGCAACGCTGGATCGCCC
GCCTGCCCGGTTTCAAAATCAGCGAAAACAAAGCCCAAGCCTTATGGGAACTCGCCGGCAAAAACATAGAAGGGCTGCAA
ACCGACAAAGCCCTCCCCACCGACTGGCAGGCGTGGCGCAGCGAACCACAAAACGCCGCCCTGCTCGAAAACCTGAAAAC
CTTCTTCGCGCAAATGCCGTCTGAAGACGAGGCGGCGCAAGGTTCAGACGGCATCAATAAAGCCGTGGCGGGCAAAACCT
TCGTGTTAACCGGCACCCTGCCCACCCTCAAACGCGACCAAGCCCAATCCCTAATCGAAGCCGCAGGCGGCAAAGTTTCC
GGCAGCGTGTCCAAAAAAACCGATTACGTCGTCGCCGGAGAAGCCGCGGGCAGCAAGCTGGAAAAAGCCAATGCCTTGGG
CGTTTCCGTCCTCAGCGAAGCCGAACTGCTTACCCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
TTTTCGGGTAAAATTCAGCTGTTTTCAACCTATCATTTTTCAGACGACCTTTGCCCGTTTCATCCCGTATCAGACAAAGG
TCGTCTGAAAACCTTCTCTC

Downstream 100 bases:

>100_bases
ACCCATGCCGTCTGAACCGCCTTCAGACGGCATTGCCCACTTTTCCGAACCACACCATGCACACCATTTCCTTTCCAAAC
CGCACCCGACTCACCGCCCT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 841; Mature: 841

Protein sequence:

>841_residues
MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEYYTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSP
TQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQAENGQKPFANPRNAAAGSLRQL
DSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYFQALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVV
KVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDV
RVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAETPSANQTQSVPLHKPYRLPTHCPICRSEIER
EEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESD
QMPSEGSSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLP
DIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPPARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQ
TDKALPTDWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGKVS
GSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG

Sequences:

>Translated_841_residues
MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEYYTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSP
TQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQAENGQKPFANPRNAAAGSLRQL
DSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYFQALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVV
KVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDV
RVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAETPSANQTQSVPLHKPYRLPTHCPICRSEIER
EEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESD
QMPSEGSSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLP
DIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPPARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQ
TDKALPTDWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGKVS
GSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG
>Mature_841_residues
MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEYYTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSP
TQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQAENGQKPFANPRNAAAGSLRQL
DSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYFQALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVV
KVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDV
RVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAETPSANQTQSVPLHKPYRLPTHCPICRSEIER
EEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESD
QMPSEGSSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLP
DIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPPARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQ
TDKALPTDWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGKVS
GSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=102, Percent_Identity=44.1176470588235, Blast_Score=76, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=645, Percent_Identity=48.8372093023256, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI87082305, Length=401, Percent_Identity=25.9351620947631, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NEIM0 (A9M2U7)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001598770.1
- ProteinModelPortal:   A9M2U7
- EnsemblBacteria:   EBNEIT00000011588
- GeneID:   5796138
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_0619
- GeneTree:   EBGT00050000020106
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2517814
- BioCyc:   NMEN374833:NMCC_0619-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 92361; Mature: 92361

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 145-145 BINDING 143-143 BINDING 166-166 BINDING 203-203 BINDING 321-321 BINDING 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEYYTLDAPSVPDAEYDKL
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
FRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHA
HHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCHH
EMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTQNIK
HHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHH
TVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQAENGQKPFANPRNAAAGSLRQL
HHHCCCEEEECCCCHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYFQALGFSLPNGNFGCFKNIDEVLAF
HHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHH
YEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVEAIDV
HHHHHHHCCCCCEECCCEEEEHHHHHHHHHCCHHHCCCCHHHHCCCCHHHHHHHHHHHHH
QIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDVIPEVVR
HCCCCCCCCHHHHCCEEEECCEEEECCEECCCHHHHHHHCCCCCEEEEEHHHHHHHHHHH
VIFERRPMRETAVAVSDGIGHRQDDLFAETPSANQTQSVPLHKPYRLPTHCPICRSEIER
HHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDLVRHFADLYRL
HHCCHHHHCCCCCEEEHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DIPTLQKMKETADKTVAESDQMPSEGSSVGASGKHKKQPVKWAENILAGIEASKTPELAR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
FLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
AMIDELLAAGVAPQTQAVTIPPARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQ
HHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCC
TDKALPTDWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTL
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEECCC
PTLKRDQAQSLIEAAGGKVSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEYYTLDAPSVPDAEYDKL
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
FRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHA
HHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCHH
EMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTQNIK
HHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHH
TVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQAENGQKPFANPRNAAAGSLRQL
HHHCCCEEEECCCCHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYFQALGFSLPNGNFGCFKNIDEVLAF
HHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHH
YEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVEAIDV
HHHHHHHCCCCCEECCCEEEEHHHHHHHHHCCHHHCCCCHHHHCCCCHHHHHHHHHHHHH
QIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDVIPEVVR
HCCCCCCCCHHHHCCEEEECCEEEECCEECCCHHHHHHHCCCCCEEEEEHHHHHHHHHHH
VIFERRPMRETAVAVSDGIGHRQDDLFAETPSANQTQSVPLHKPYRLPTHCPICRSEIER
HHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDLVRHFADLYRL
HHCCHHHHCCCCCEEEHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DIPTLQKMKETADKTVAESDQMPSEGSSVGASGKHKKQPVKWAENILAGIEASKTPELAR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
FLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
AMIDELLAAGVAPQTQAVTIPPARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQ
HHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCC
TDKALPTDWQAWRSEPQNAALLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTL
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEECCC
PTLKRDQAQSLIEAAGGKVSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA