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The map label for this gene is amtB [H]

Identifier: 161869547

GI number: 161869547

Start: 584036

End: 585340

Strand: Reverse

Name: amtB [H]

Synonym: NMCC_0562

Alternate gene names: 161869547

Gene position: 585340-584036 (Counterclockwise)

Preceding gene: 161869548

Following gene: 161869537

Centisome position: 27.18

GC content: 57.39

Gene sequence:

>1305_bases
ATGAAAAAACACATATGGGCGGCATCTTTGCTGCCGACATCCCTATCGGCAGAACCTTTAAACTGGTGGAAGCCTTATTC
CGCCGTCAATTCGGGCGATACCGCCTGGGTGATGACTGCGGCTGCCTTGGTACTGTTGATGACGCTTCCCGGGCTGGCTT
TATTCTACGGCGGTATGGTGCGGAAAAAAAACCTGCTCTCGACGATGATGCACAGCTTTTCCATCGCGACATTGGTGGGC
ATCCTTTGGGTCGCCGTCGGCTATTCTTTAGCGTTCACGCCGGGAAATGCCTTTATCGGCGGTTTGGGGCGCGTATTTTT
AAGCGGGATGCAGATAGACGCTGCCGCACAGATGCTGACCGTGTCGCCCAATGCGCCGACTGTTCCCGAACCGGTATTTA
TGTTTTTTCAGATGACGTTTGCCATTATTTCGACCGCCATTATTACCGGCGCGTTTGCCGAACGGATGAAATATTCGGCA
ATGATGCTGTTTTCGGGCATATGGTTTTTATTGGTTTATGTGCCGGGCGCGCATTGGGTCTGGGGCGGCGGCTTTATGAG
CAAGGGCGGCGTATTGGATTATGCCGGCGGTACGGTGGTGCACATCAATGCCGGTATCGCGGGACTCGTCGCCGCCTTGG
TTTTGGGCAGGCGCATAGGCTACGGGCGCGAGGCGATGCCTCCGCACAATATGGCGATGACACTGATCGGCGCGGCAATG
TTGTGGTTCGGCTGGTTCGGCTTTAACGCCGGATCGGCGCTTGCGGCAGACGCGGCGGCGGGTATGGCGATGGCGGTAAC
GCAGGTGTCGGCCGTATTCGGCGCGGCAGGCTGGCTTGCCTGCGAAAAAATAGCGGGACACAAACCTTCCGCTTTGGGGC
TGGCTTCCGGCGCGGTTTCCGGTCTGGTCGGCATCACCCCTGCCGCCGGCTTTACCGGCCCGTCGGGCGCGGCCGCCATC
GGTATATTGACTGCCGCCGCGTGCTTTGTGTCCGTCACCGTCGTCAAACACAAATTGCGTTACGATGATTCTTTGGACGC
TTTCGGCATACACGGATTCGGCGGGCTGGTGGGCGGAATATTGACCGGCATCTTTTTCGACAACCGCATTTTCGGCGGGG
ATGCGGCAGTTTGGCAGCAGTTGTGGATACAGGTAAAAGACGGGTTCGTTATGGCGGCATACAGCGGGCTGATGAGTTGG
GCGATTTTGAAGGTCGTGGGGAAAATCTGCGGCGGCCTGCGCGTCGGCAAGGATGTCGAACGCGAAGGTTTGGATCTGAA
TATCCACGGCGAACGCGTGGAATAA

Upstream 100 bases:

>100_bases
CGCGCCGCGCCGGTCTGGCAGGGCTTGCGTCCCTCCCGGACAAGCCCCGACCCCGCCTTTCCGAAAGACGGGCTCAACCA
TTAAGGAAACTTTAATCAAA

Downstream 100 bases:

>100_bases
GGGCGGCTATGCCGTCTGAAGCCTGAAAATCGGGTTTCAGACGGCATTTTTCACGTTTGCCGCCGATGGATAAACATATA
GTGGATTAACAAAAATCAGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALVLLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVG
ILWVAVGYSLAFTPGNAFIGGLGRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRRIGYGREAMPPHNMAMTLIGAAM
LWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAAGWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAI
GILTAAACFVSVTVVKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMAAYSGLMSW
AILKVVGKICGGLRVGKDVEREGLDLNIHGERVE

Sequences:

>Translated_434_residues
MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALVLLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVG
ILWVAVGYSLAFTPGNAFIGGLGRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRRIGYGREAMPPHNMAMTLIGAAM
LWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAAGWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAI
GILTAAACFVSVTVVKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMAAYSGLMSW
AILKVVGKICGGLRVGKDVEREGLDLNIHGERVE
>Mature_434_residues
MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALVLLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVG
ILWVAVGYSLAFTPGNAFIGGLGRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRRIGYGREAMPPHNMAMTLIGAAM
LWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAAGWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAI
GILTAAACFVSVTVVKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMAAYSGLMSW
AILKVVGKICGGLRVGKDVEREGLDLNIHGERVE

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=436, Percent_Identity=41.9724770642202, Blast_Score=323, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32564042, Length=426, Percent_Identity=27.2300469483568, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=27.5395033860045, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=447, Percent_Identity=25.503355704698, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=26.1160714285714, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=35.6459330143541, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324187, Length=342, Percent_Identity=39.4736842105263, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=32.9326923076923, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI161078314, Length=425, Percent_Identity=29.1764705882353, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45401; Mature: 45401

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALVLLMTLPGLALFYGGMV
CCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
RKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGLGRVFLSGMQIDAAAQMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCHHEEEE
VSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSAMMLFSGIWFLLVYVPGAHWV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
WGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRRIGYGREAMPPHNMAMTLIGAAM
ECCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAAGWLACEKIAGHKPSALGLASGAVS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GLVGITPAAGFTGPSGAAAIGILTAAACFVSVTVVKHKLRYDDSLDAFGIHGFGGLVGGI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMAAYSGLMSWAILKVVGKICGGLRVGKDVE
HHHHHHCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
REGLDLNIHGERVE
HCCCEEEECCCCCC
>Mature Secondary Structure
MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALVLLMTLPGLALFYGGMV
CCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
RKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGLGRVFLSGMQIDAAAQMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCHHEEEE
VSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSAMMLFSGIWFLLVYVPGAHWV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
WGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRRIGYGREAMPPHNMAMTLIGAAM
ECCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAAGWLACEKIAGHKPSALGLASGAVS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
GLVGITPAAGFTGPSGAAAIGILTAAACFVSVTVVKHKLRYDDSLDAFGIHGFGGLVGGI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMAAYSGLMSWAILKVVGKICGGLRVGKDVE
HHHHHHCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
REGLDLNIHGERVE
HCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]